Repair of larynx, trachea, and other fibrocartilaginous tissues
... herein. An initial alignment can be re?ned by comparison to
a multi-sequence alignment of a family of related proteins.
Once the alignment betWeen the candidate sequence and the
seven-cysteine domain is made and re?ned, a percent
homology score is calculated. The aligned amino acid resi
dues of the ...
A STUDY OF THE ROLES OF SELECTED ARGININE AND
... indicated by the arrow. The cleavage site of the activation peptide is indicated by the
underlined arrow. The triple lysine residues which are unique to TAFI are bold.
Numbering starts with the first residue of the pro-enzyme TAFI, after the 22-amino acid
pre-peptide. Cleavage of TAFI at Arg 92 by t ...
... Printed in the United States of America on acid-free paper
Chapter 4 - University of Amsterdam
... levels of carnitine, while food sources derived from plants contribute very little (26).
Although carnitine can be obtained from the diet, most mammalian species are also
able to synthesize it endogenously. The carnitine biosynthetic pathway was chemically
characterized in the 1970s in rat and the ﬁ ...
Silaffins in Silica Biomineralization and Biomimetic Silica
... nanostructured silica cell walls of diatoms is reasonable. Besides the cingulins other biomolecules have
been found to be associated with diatom cell walls and to be able to precipitate silica from a solution of
silicic acid, specifically the silaffins and long chain polyamines (LCPAs).
LCPAs are ma ...
CHAPTER 1 INTRODUCTION
... them as a hay crop. It was first cultivated in South Africa in 1903
at Cedara in Natal (Cronje , 1997). It was not until after 1945 that
their value as a supplier of feed and food oil was recognised and
exploited (Snyder and Kwon , 1987). In South Africa a genetically
improved soya bean with increas ...
Characterization of an Immuno-dominant Variable Surface
... an adenine residue 17 by 5' of the start codon by primer extension sequence analysis using oligonucleotide SR09 (data
not shown) . 5' untranslated regions of actin (11 bp) (36, 44)
and ferredoxin (9 bp) (45) genes were likewise very short as
compared with other eukaryotic gene transcripts . A common ...
glucagon superfamily peptides exhibiting nuclear hormone
... different tissues to form a number of different proglucagon-derived peptides, including glucagon,
glucagon-like peptide-1 (GLP-1), glucagon-like peptide-2 (GLP-2) and oxyntomodulin (OXM),
that are involved in a wide variety of physiological functions, including glucose homeostasis,
insulin secretion ...
Pyruvate dehydrogenase - Wageningen UR E
... 1984b; Packman & Perham, 1987; Chuang, 1985;Hanemaaijer et al., 1987].From N- to Cterminus it contains two or three lipoyl domains (dependent on the type and source of the
complex), an E1/E3 binding domain and a catalytic domain. NMR experiments indicate that
the linker sequences are extended and hi ...
Safety Assessment of Palmitoyl Oligopeptides as Used in Cosmetics
... Studies relating to palmitoyl oligopeptide-induced skin rejuvenation are included in Table 3.
The anti-wrinkle effect, due to increased collagen synthesis, of palmitoyl tripeptide-1 (palmitoyl-Gly-His-Lys) was
evaluated in a blind, vehicle-controlled test involving 15 female s ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... (1,2). The L10 protein binds to ribosomal RNA (rRNA)
(nucleotides 1030–1124 in Escherichia coli) next to the
protein L11 at the base of the stalk (3,4). L12 is the only
multicopy protein on the ribosome (5), its number varying
from species to species (1). In E. coli, two L12 dimers and
one L10 prote ...
... : Identified by targeted liquid chromatography LC-MS/MS; ˝: Identified by non-targeted LC-MS/MS and : Confirmed by LC-MS/MS.
Evidence for Several Cysteine Transport Mechanisms in the
... electron transport chain, has limited permeability to many metabolites that require specialized membrane transport proteins,
most of which remain unknown to date. To minimize the interference by the OMM with the rate of IMM cysteine uptake, we
examined the characteristics of cysteine transport in th ...
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed
... orchids, Vanilla planifolia and V. tahitensis are the source
of natural vanilla1, although trace amounts of vanillin can be
found in a variety of different plant species scattered in the plant
kingdom2. Vanillin (3-methoxy-4-hydroxybenzaldehyde) is the
main ﬂavour component of vanilla extract from c ...
... ATP-binding cassette transporters (ABC-transporter) are members of a protein superfamily that is one of
the largest and most ancient families with representatives in all extant phyla from prokaryotes to humans.
ABC transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to ...
Regulation of Phenylalanine and Tyrosine
... The chorismate mutase-prephenate de hydratase I aggregate and prephenate dehydratase II
had molecular weights of 75000 and 30000, respectively.
For P. aeruginosa strain P A 0 1 , the molecular weights of the corresponding enzymes
determined by the same method were : prephenate dehydrogenase, 150000; ...
Hydrolyzed Source Proteins as Used in Cosmetics
... plasma by food-grade microbial proteases and aided with heat denaturation.15 The maximum degree of hydrolysis
was 43% at an enzyme concentration of 110 LAPU/g protein after 15.5 h. The resultant substrate consists of small
peptides (molecular masses were less than 6.5 kDa and most were less than 1.0 ...
Structure and Function of Thymosin β4
... digestion gave two peptides which lacked a free α-amino group. These two peptides were
deduced to be a part of the N-terminus region. The tryptic digestion also produced a
peptide which lacks a lysine residue and was therefore placed in the C-terminus region.
The side chains were assigned their posi ...
Effects of low crude-protein diets fortified with crystalline amino acids
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of
protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids
in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM
diet is different from ...
The Role of N- and C-terminal Amino Acids to
... thesis. Without his time and advice, this project would not have been possible. I would
now like to thank my parents for their support throughout my seven years of postsecondary education. You were always there for me when I needed additional
encouragement and I am forever grateful. Thank you to my ...
Ribosomally synthesized and post-translationally modified peptides
Ribosomally synthesized and post-translationally modified peptides (RiPPs), also known as ribosomal natural products, are a diverse class of natural products of ribosomal origin. Consisting of more than 20 sub-classes, RiPPs are produced by a variety of organisms, including prokaryotes, eukaryotes, and archaea, and they possess a wide range of biological functions.As a consequence of the falling cost of genome sequencing and the accompanying rise in available genomic data, scientific interest in RiPPs has increased in the last few decades. Because the chemical structures of RiPPs are more closely predictable from genomic data than are other natural products (e.g. alkaloids, terpenoids), their presence in sequenced organisms can, in theory, be identified rapidly. This makes RiPPs an attractive target of modern natural product discovery efforts.