Conformational changes in actin–myosin isoforms probed by Ni(II
... primary aromatic amino acids (Brown et al., 1995), making it a very attractive reagent since most available zero-length protein cross-linkers involve nucleophilic amino acids. As an example, Ni(II)Gly–Gly–His was used to investigate the hydrophobic interface between actin and skeletal muscle myosin ...
... primary aromatic amino acids (Brown et al., 1995), making it a very attractive reagent since most available zero-length protein cross-linkers involve nucleophilic amino acids. As an example, Ni(II)Gly–Gly–His was used to investigate the hydrophobic interface between actin and skeletal muscle myosin ...
biolsci.org - International Journal of Biological Sciences
... Dxs catalyses the first step of thiazole biosynthesis by condensation of glyceraldehyde 3-phosphate and pyruvate to produce DXP (Figure 1a) [49]. This step also participates in the biosynthesis of pyridoxol (vitamin B6) and isoprenoids [50, 51]. Intriguingly, ThDP is both the product of Dxp and the ...
... Dxs catalyses the first step of thiazole biosynthesis by condensation of glyceraldehyde 3-phosphate and pyruvate to produce DXP (Figure 1a) [49]. This step also participates in the biosynthesis of pyridoxol (vitamin B6) and isoprenoids [50, 51]. Intriguingly, ThDP is both the product of Dxp and the ...
Specific and ubiquitous expression of different Zn finger protein
... In addition.the Tyr/Phe,Phe,Leu and the H-C link sequence TGEKP is also conserved. Structural analysis of mkr3.mkr4 and mkr5 Mkr3 contains a total of 428 amino acids including 14 full and 1 partial fingers(fig.1 and 2). At the 3' end.there is a termination codon at nt. 1307 preceeded by 27 unique no ...
... In addition.the Tyr/Phe,Phe,Leu and the H-C link sequence TGEKP is also conserved. Structural analysis of mkr3.mkr4 and mkr5 Mkr3 contains a total of 428 amino acids including 14 full and 1 partial fingers(fig.1 and 2). At the 3' end.there is a termination codon at nt. 1307 preceeded by 27 unique no ...
1 INTRODUCTION
... „Microparticles‟ is a collective name for microcapsules and microspheres, which are colloidal microstructures with size 1–250 µm (reviewed by Allemann, Leroux and Gurny, 1998; Reis, Neufeld, Ribeiro and Veiga, 2006). These microstructures can be made from synthetic polymers, natural biodegradable po ...
... „Microparticles‟ is a collective name for microcapsules and microspheres, which are colloidal microstructures with size 1–250 µm (reviewed by Allemann, Leroux and Gurny, 1998; Reis, Neufeld, Ribeiro and Veiga, 2006). These microstructures can be made from synthetic polymers, natural biodegradable po ...
Lactococcus lactis as expression host for the biosynthetic
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
CALPAIN: TRANSITIONING FROM THE USE OF THE
... Figure 2.2. The effect of the stability of α7-helix on Trp106 (purple) in mI-II (A), µI-II (B), mcalpain (C) and mini-calpain 8 (D).. .................................................................................... 26 Figure 2.3. Autolysis assay of mini-calpain 8................................. ...
... Figure 2.2. The effect of the stability of α7-helix on Trp106 (purple) in mI-II (A), µI-II (B), mcalpain (C) and mini-calpain 8 (D).. .................................................................................... 26 Figure 2.3. Autolysis assay of mini-calpain 8................................. ...
Insights into interactions between poly(ethylene glycol) and proteins
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
Lecture 1 - "Hudel" Luecke
... This is the sequence of hexokinase, yeast hexokinase from the yeast species Saccharomyces cerevisiae to be specific. To find out more about this protein, jump to the Brookhaven Protein Data Bank 3D browser and enter hexokinase in the textbox, or SCOP (Structural Classification of Proteins) and use t ...
... This is the sequence of hexokinase, yeast hexokinase from the yeast species Saccharomyces cerevisiae to be specific. To find out more about this protein, jump to the Brookhaven Protein Data Bank 3D browser and enter hexokinase in the textbox, or SCOP (Structural Classification of Proteins) and use t ...
BIOCHEMISTRY
... Hydrocarbons $30 What is the removing of water to join monomers called? What is the addition of water to split polymers called? Dehydration synthesis Hydrolysis Cinda Sheldon ...
... Hydrocarbons $30 What is the removing of water to join monomers called? What is the addition of water to split polymers called? Dehydration synthesis Hydrolysis Cinda Sheldon ...
Src protein–tyrosine kinase structure and regulation
... illustrate the inferred catalytic properties of Src. Lys295 represents an invariant residue of protein kinases that forms ion pairs with the a- and b-phosphates of ATP (Table 1). Asp386 orients the tyrosyl group of the substrate protein in a catalytically competent state. Asp386 may function as a ba ...
... illustrate the inferred catalytic properties of Src. Lys295 represents an invariant residue of protein kinases that forms ion pairs with the a- and b-phosphates of ATP (Table 1). Asp386 orients the tyrosyl group of the substrate protein in a catalytically competent state. Asp386 may function as a ba ...
Class I tRNA
... Both 30S and 50S subunits are self-assembled in vitro. In 30S subunit, S4 and S8 bind to 16S rRNA first, other proteins then join sequentially and cooperatively. ...
... Both 30S and 50S subunits are self-assembled in vitro. In 30S subunit, S4 and S8 bind to 16S rRNA first, other proteins then join sequentially and cooperatively. ...
The PRT protein family Sangita C Sinha* and Janet L Smith
... The available structures provide a wealth of information on states of PRTases relevant to catalysis, effectively providing snapshots along the reaction pathway [31,40]. The closed-loop structures, in particular, provide a remarkably consistent picture of Mg•PRPP binding. Mg•PRPP binds in a virtually ...
... The available structures provide a wealth of information on states of PRTases relevant to catalysis, effectively providing snapshots along the reaction pathway [31,40]. The closed-loop structures, in particular, provide a remarkably consistent picture of Mg•PRPP binding. Mg•PRPP binds in a virtually ...
Isoleucine Synthesis by Clostridium sporogenes from
... L-ubelling and fractionation of cells. 14C-Labelled compounds were added to exponentially growing cells (10 ml cultures, ODbo0= 0-3),incubated for 4 h (about one generation) and harvested by centrifugation. Cell pellets were extracted twice at 0 "C with 5 ml 10% (w/v) TCA for 15 min, then for 30 min ...
... L-ubelling and fractionation of cells. 14C-Labelled compounds were added to exponentially growing cells (10 ml cultures, ODbo0= 0-3),incubated for 4 h (about one generation) and harvested by centrifugation. Cell pellets were extracted twice at 0 "C with 5 ml 10% (w/v) TCA for 15 min, then for 30 min ...
Transamination Governs Nitrogen Isotope Heterogeneity of Amino
... The nitrogen isotope composition (δ N) of organisms provides information on trophic systems.1,2 While the bulk δ15N of tissues indicates the trophic position of organisms,3,4 the δ15N of specific compounds has the potential to provide more detailed insights, because different compounds differ in biosyn ...
... The nitrogen isotope composition (δ N) of organisms provides information on trophic systems.1,2 While the bulk δ15N of tissues indicates the trophic position of organisms,3,4 the δ15N of specific compounds has the potential to provide more detailed insights, because different compounds differ in biosyn ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... allele, altering potential phosphorylation and ubiquitination sites. No significant effect was seen on the rate of glucose-induced degradation of these mutant proteins. Deletion mutations were constructed, removing residues 2-30, 31-60, 61-90, and 49-78 of the N-terminal cytoplasmic domain, as well ...
... allele, altering potential phosphorylation and ubiquitination sites. No significant effect was seen on the rate of glucose-induced degradation of these mutant proteins. Deletion mutations were constructed, removing residues 2-30, 31-60, 61-90, and 49-78 of the N-terminal cytoplasmic domain, as well ...
Amino Acids
... A. Types of collagen • Collagen superfamily of proteins include > 20 collagen types, as well as additional proteins that have collagenlike domains. • The 3 polyp. α-chains are held together by H-bonds b/w the chains. • Variations in aa sequence of α-chains result in structural components that are ~ ...
... A. Types of collagen • Collagen superfamily of proteins include > 20 collagen types, as well as additional proteins that have collagenlike domains. • The 3 polyp. α-chains are held together by H-bonds b/w the chains. • Variations in aa sequence of α-chains result in structural components that are ~ ...
post- translational modifications
... We are pleased to present a selection of our building blocks for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practicall ...
... We are pleased to present a selection of our building blocks for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practicall ...
Gene Section GAST (gastrin) Atlas of Genetics and Cytogenetics
... and G34 (10%). Although G17 is the predominant product, G34 is the major circulating form of gastrin due to its slower rate of clearance. In both humans, the half-life of circulating G34 is approximately five times longer than that of G17. The proteolytic processing of progastrin involves convertase ...
... and G34 (10%). Although G17 is the predominant product, G34 is the major circulating form of gastrin due to its slower rate of clearance. In both humans, the half-life of circulating G34 is approximately five times longer than that of G17. The proteolytic processing of progastrin involves convertase ...
Two nearly identical terpene synthases catalyze the
... snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawberry nerolidol/linalool synthase (FaNES; CAD57106) and Arabidopsis linalool synthase (AAO85533). The alignment was performed using CLUSTALW, and was shaded with BoxShade version 3.21 software (Human Genome Sequencing Ce ...
... snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawberry nerolidol/linalool synthase (FaNES; CAD57106) and Arabidopsis linalool synthase (AAO85533). The alignment was performed using CLUSTALW, and was shaded with BoxShade version 3.21 software (Human Genome Sequencing Ce ...
Molecular signatures-based prediction of enzyme
... At the molecular level, several structural mechanisms have been related to enzyme promiscuity. For instance, promiscuous binding has been observed in a large hydrophobic cleft or cavity or in a region with some other unusual structural feature (Copley, 2003). Similarly, promiscuity has been linked i ...
... At the molecular level, several structural mechanisms have been related to enzyme promiscuity. For instance, promiscuous binding has been observed in a large hydrophobic cleft or cavity or in a region with some other unusual structural feature (Copley, 2003). Similarly, promiscuity has been linked i ...
PDF - Geoff McFadden`s Lab
... equivalents is unclear. Similarly, no enzymes for a pentose phosphate pathway have been found in apicoplasts. Plant plastids can import ATP in exchange for ADP using an antiporter that is similar to that of the human pathogen Rickettsia 30, but no such transporter has been found in Plasmodium specie ...
... equivalents is unclear. Similarly, no enzymes for a pentose phosphate pathway have been found in apicoplasts. Plant plastids can import ATP in exchange for ADP using an antiporter that is similar to that of the human pathogen Rickettsia 30, but no such transporter has been found in Plasmodium specie ...
allyl cysteine sulphoxide
... callus have been maintained for up to 15 days on a phytogel/MS medium, with and without sulphate, containing a range of potential precursors to the synthesis of Alliin (at different concentrations). This method of substrate feeding will only give a positive result if: the substrate gets into the ...
... callus have been maintained for up to 15 days on a phytogel/MS medium, with and without sulphate, containing a range of potential precursors to the synthesis of Alliin (at different concentrations). This method of substrate feeding will only give a positive result if: the substrate gets into the ...