Conformational changes in actin–myosin isoforms probed by Ni(II
... primary aromatic amino acids (Brown et al., 1995),
making it a very attractive reagent since most available
zero-length protein cross-linkers involve nucleophilic
amino acids. As an example, Ni(II)Gly–Gly–His was
used to investigate the hydrophobic interface between
actin and skeletal muscle myosin ...
biolsci.org - International Journal of Biological Sciences
... Dxs catalyses the first step of thiazole biosynthesis by condensation of glyceraldehyde 3-phosphate
and pyruvate to produce DXP (Figure 1a) . This
step also participates in the biosynthesis of pyridoxol
(vitamin B6) and isoprenoids [50, 51]. Intriguingly,
ThDP is both the product of Dxp and the ...
Specific and ubiquitous expression of different Zn finger protein
... In addition.the Tyr/Phe,Phe,Leu and the H-C link sequence TGEKP is also
Structural analysis of mkr3.mkr4 and mkr5
Mkr3 contains a total of 428 amino acids including 14 full and 1
partial fingers(fig.1 and 2). At the 3' end.there is a termination codon at
nt. 1307 preceeded by 27 unique no ...
... „Microparticles‟ is a collective name for microcapsules and microspheres, which are colloidal
microstructures with size 1–250 µm (reviewed by Allemann, Leroux and Gurny, 1998; Reis,
Neufeld, Ribeiro and Veiga, 2006). These microstructures can be made from synthetic
polymers, natural biodegradable po ...
Lactococcus lactis as expression host for the biosynthetic
... strains to stimulate the incorporation of Trp analogues .
This approach was explored for L. lactis PA1001 in CDM. A
concentration of 0.2 mg/ml of IAA completely inhibited the
growth of L. lactis PA1001 (Figure 3A). However, addition of
Trp did not elevate the IAA-inhibitory effect, suggesting th ...
CALPAIN: TRANSITIONING FROM THE USE OF THE
... Figure 2.2. The effect of the stability of α7-helix on Trp106 (purple) in mI-II (A), µI-II (B), mcalpain (C) and mini-calpain 8 (D).. .................................................................................... 26
Figure 2.3. Autolysis assay of mini-calpain 8................................. ...
Lecture 1 - "Hudel" Luecke
... This is the sequence of hexokinase, yeast hexokinase from the yeast species Saccharomyces cerevisiae
to be specific. To find out more about this protein, jump to the Brookhaven Protein Data Bank 3D
browser and enter hexokinase in the textbox, or SCOP (Structural Classification of Proteins) and use t ...
... Hydrocarbons $30
What is the removing of water to
join monomers called?
What is the addition of water to
split polymers called?
Src protein–tyrosine kinase structure and regulation
... illustrate the inferred catalytic properties of Src. Lys295
represents an invariant residue of protein kinases that
forms ion pairs with the a- and b-phosphates of ATP
(Table 1). Asp386 orients the tyrosyl group of the substrate protein in a catalytically competent state.
Asp386 may function as a ba ...
Class I tRNA
... Both 30S and 50S subunits are self-assembled in vitro. In
30S subunit, S4 and S8 bind to 16S rRNA first, other proteins
then join sequentially and cooperatively.
The PRT protein family Sangita C Sinha* and Janet L Smith
... The available structures provide a wealth of information
on states of PRTases relevant to catalysis, effectively
providing snapshots along the reaction pathway [31,40].
The closed-loop structures, in particular, provide a
remarkably consistent picture of Mg•PRPP binding.
Mg•PRPP binds in a virtually ...
Isoleucine Synthesis by Clostridium sporogenes from
... L-ubelling and fractionation of cells. 14C-Labelled compounds were added to exponentially growing cells (10 ml
cultures, ODbo0= 0-3),incubated for 4 h (about one generation) and harvested by centrifugation. Cell pellets were
extracted twice at 0 "C with 5 ml 10% (w/v) TCA for 15 min, then for 30 min ...
Transamination Governs Nitrogen Isotope Heterogeneity of Amino
... The nitrogen isotope composition (δ N) of organisms
provides information on trophic systems.1,2 While the bulk
δ15N of tissues indicates the trophic position of organisms,3,4
the δ15N of speciﬁc compounds has the potential to provide
more detailed insights, because diﬀerent compounds diﬀer in
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... allele, altering potential phosphorylation and ubiquitination sites. No significant effect was seen on the
rate of glucose-induced degradation of these mutant proteins. Deletion mutations were constructed, removing
residues 2-30, 31-60, 61-90, and 49-78 of the N-terminal cytoplasmic domain, as well ...
... A. Types of collagen
• Collagen superfamily of proteins include > 20 collagen
types, as well as additional proteins that have collagenlike domains.
• The 3 polyp. α-chains are held together by H-bonds b/w
• Variations in aa sequence of α-chains result in structural
components that are ~ ...
post- translational modifications
... We are pleased to present a selection of our building blocks
for the synthesis of PTM peptides and proteins.
Post-translational modifications (PTMs) enable the cell
to change the properties and activities of proteins after
expression. Most of the PTMs are catalyzed by specialized
enzymes. Practicall ...
Gene Section GAST (gastrin) Atlas of Genetics and Cytogenetics
... and G34 (10%). Although G17 is the predominant
product, G34 is the major circulating form of gastrin
due to its slower rate of clearance. In both humans, the
half-life of circulating G34 is approximately five times
longer than that of G17.
The proteolytic processing of progastrin involves
Two nearly identical terpene synthases catalyze the
... snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawberry nerolidol/linalool synthase (FaNES; CAD57106) and Arabidopsis
linalool synthase (AAO85533). The alignment was performed using CLUSTALW, and was shaded with BoxShade version 3.21 software (Human Genome Sequencing
Molecular signatures-based prediction of enzyme
... At the molecular level, several structural mechanisms have been
related to enzyme promiscuity. For instance, promiscuous binding
has been observed in a large hydrophobic cleft or cavity or in a region
with some other unusual structural feature (Copley, 2003). Similarly,
promiscuity has been linked i ...
PDF - Geoff McFadden`s Lab
... equivalents is unclear. Similarly, no enzymes for a pentose phosphate pathway have been found in apicoplasts.
Plant plastids can import ATP in exchange for ADP
using an antiporter that is similar to that of the human
pathogen Rickettsia 30, but no such transporter has been
found in Plasmodium specie ...
allyl cysteine sulphoxide
... callus have been maintained for up to
15 days on a phytogel/MS medium, with
and without sulphate, containing a range
of potential precursors to the synthesis
of Alliin (at different concentrations).
This method of substrate feeding will
only give a positive result if:
the substrate gets into the ...
Ribosomally synthesized and post-translationally modified peptides
Ribosomally synthesized and post-translationally modified peptides (RiPPs), also known as ribosomal natural products, are a diverse class of natural products of ribosomal origin. Consisting of more than 20 sub-classes, RiPPs are produced by a variety of organisms, including prokaryotes, eukaryotes, and archaea, and they possess a wide range of biological functions.As a consequence of the falling cost of genome sequencing and the accompanying rise in available genomic data, scientific interest in RiPPs has increased in the last few decades. Because the chemical structures of RiPPs are more closely predictable from genomic data than are other natural products (e.g. alkaloids, terpenoids), their presence in sequenced organisms can, in theory, be identified rapidly. This makes RiPPs an attractive target of modern natural product discovery efforts.