A structural comparison of molybdenum cofactor
... including E. coli [18], R. sphaeroides [19] and R. capsulatus [20]. DMSO reductase from R. sphaeroides is a soluble periplasmic single subunit protein comprising 780 residues which contains no cofactor other than Mo-co. In contrast, the E. coli enzyme is an integral membrane protein consisting of th ...
... including E. coli [18], R. sphaeroides [19] and R. capsulatus [20]. DMSO reductase from R. sphaeroides is a soluble periplasmic single subunit protein comprising 780 residues which contains no cofactor other than Mo-co. In contrast, the E. coli enzyme is an integral membrane protein consisting of th ...
Isolation and Characterization of Protease Inhibitors from Animal
... serine proteases have been separated (Yanes et al. 2005). The majority of these H. medicinalis serine proteases are found in different leech trypsin inhibitor families. However, other inhibitors are similar to other families. For instance, the 3,128-Da inhibitor demonstrates a well conserved region ...
... serine proteases have been separated (Yanes et al. 2005). The majority of these H. medicinalis serine proteases are found in different leech trypsin inhibitor families. However, other inhibitors are similar to other families. For instance, the 3,128-Da inhibitor demonstrates a well conserved region ...
Recombinant expression and characterisation of monofunctional S-
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
Preference for and learning of amino acids in larval
... amino acids and how behaviour is organized accordingly. This is a significant gap in our knowledge because amino acids are required for protein synthesis − and hence for life as we know it. Choosing Drosophila larvae as a case study, we provide the first systematic analysis of both the preference be ...
... amino acids and how behaviour is organized accordingly. This is a significant gap in our knowledge because amino acids are required for protein synthesis − and hence for life as we know it. Choosing Drosophila larvae as a case study, we provide the first systematic analysis of both the preference be ...
Structure and function of the eukaryotic ADP
... reaction converting glucose and ADP to glucose-6-phosphate and AMP. The enzyme is well studied in extremophilic archaea, where ADPGK is part of a set of glycolytic enzymes that use ADP instead of ATP for the phosphorylation of various sugars. However, ADPGK has also been found in the genomes of meso ...
... reaction converting glucose and ADP to glucose-6-phosphate and AMP. The enzyme is well studied in extremophilic archaea, where ADPGK is part of a set of glycolytic enzymes that use ADP instead of ATP for the phosphorylation of various sugars. However, ADPGK has also been found in the genomes of meso ...
University of Groningen Transport processes in penicillin
... Penicillins and cephalosporins belong to the large and complex family of $lactam antibiotics which members possess one common structural motif, the fourmembered $-lactam ring (Table 1.1) [11, 74, 139, 188, 181]. The $-lactam ring enables these antibiotics to selectively inhibit enzymes involved in t ...
... Penicillins and cephalosporins belong to the large and complex family of $lactam antibiotics which members possess one common structural motif, the fourmembered $-lactam ring (Table 1.1) [11, 74, 139, 188, 181]. The $-lactam ring enables these antibiotics to selectively inhibit enzymes involved in t ...
SBT301 - SibTech, Inc.
... Radiolabeling with Tc and other applications: After treatment with equimolar amounts of DTT, thiol group in Cys-tag 99m becomes available for direct radiolabeling with Tc for SPECT imaging of VEGF receptors (1) or site-directed conjugation of various payloads, including radionuclide chelators (2, 3) ...
... Radiolabeling with Tc and other applications: After treatment with equimolar amounts of DTT, thiol group in Cys-tag 99m becomes available for direct radiolabeling with Tc for SPECT imaging of VEGF receptors (1) or site-directed conjugation of various payloads, including radionuclide chelators (2, 3) ...
AMP-forming acetyl-CoA synthetases in Archaea show
... catalase (232 kDa), ferritin (440 kDa) and blue dextran (2000 kDa). Protein samples (0.2 ml) were loaded onto the column pre-equilibrated with 50 mM Tris (pH 7.5) containing 150 mM KCl and the column was developed at a flow rate of 0.5 mlmin –1. The subunit molecular mass of each enzyme was determin ...
... catalase (232 kDa), ferritin (440 kDa) and blue dextran (2000 kDa). Protein samples (0.2 ml) were loaded onto the column pre-equilibrated with 50 mM Tris (pH 7.5) containing 150 mM KCl and the column was developed at a flow rate of 0.5 mlmin –1. The subunit molecular mass of each enzyme was determin ...
2nd_lecture
... • Why are chaperones needed if the information for folding is inherent in the sequence? – to protect nascent proteins from the concentrated protein matrix in the cell and perhaps to accelerate slow steps ...
... • Why are chaperones needed if the information for folding is inherent in the sequence? – to protect nascent proteins from the concentrated protein matrix in the cell and perhaps to accelerate slow steps ...
Biosynthesis of `essential` amino acids by
... a high probability of being incorporated into all newly synthesized amino acids. Glutamic acid is the direct precursor of several other amino acids via the tricarboxylic acid cycle, from where, like glucose, its carbon atoms have a high probability of being incorporated into newly synthesized amino ...
... a high probability of being incorporated into all newly synthesized amino acids. Glutamic acid is the direct precursor of several other amino acids via the tricarboxylic acid cycle, from where, like glucose, its carbon atoms have a high probability of being incorporated into newly synthesized amino ...
Chapter 5 Slides
... The Sequence of Amino Acids in a Protein • is a unique characteristic of every protein • is encoded by the nucleotide sequence of DNA • is thus a form of genetic information • is read from the amino terminus to the ...
... The Sequence of Amino Acids in a Protein • is a unique characteristic of every protein • is encoded by the nucleotide sequence of DNA • is thus a form of genetic information • is read from the amino terminus to the ...
Prediction of Folding, Stability and Structure of Proteins from Amino
... understood. The specific structures and substructures a protein ultimately forms, plays an important role in how the protein will function in the cell. Basic protein structure is determined by the elemental components of the amino acids and can be described using a four level hierarchy. Proteins are ...
... understood. The specific structures and substructures a protein ultimately forms, plays an important role in how the protein will function in the cell. Basic protein structure is determined by the elemental components of the amino acids and can be described using a four level hierarchy. Proteins are ...
Journal of Microbiology and Biotechnology
... classified according to their intermediates, were reported in the bacteria [22, 30]. Some reports on the production of IAA by Bacillus species have been cited, but little is known about the IAA biosynthetic pathways in Bacillus spp. regarding the genetic basis involving the key enzymes of this proce ...
... classified according to their intermediates, were reported in the bacteria [22, 30]. Some reports on the production of IAA by Bacillus species have been cited, but little is known about the IAA biosynthetic pathways in Bacillus spp. regarding the genetic basis involving the key enzymes of this proce ...
1 Causality, Transfer Entropy and Allosteric
... between contacting residues. The method is based on the transfer entropy concept introduced recently by Schreiber, and shows that knowing only the energy landscape is not sufficient to predict information transfer and allosteric communication, and that time delayed correlations are necessary. The al ...
... between contacting residues. The method is based on the transfer entropy concept introduced recently by Schreiber, and shows that knowing only the energy landscape is not sufficient to predict information transfer and allosteric communication, and that time delayed correlations are necessary. The al ...
Processing of the Presequence of the Schizosaccharomyces pombe
... mitochondria, into S. pombe mitochondria (Fig. 3C). At first glance only intermediate length protein could be observed, but upon closer examination of the autoradiographs small amounts of mature length protein were detected. To show this more clearly, an altered S. cerevisiae iron-sulfur protein in ...
... mitochondria, into S. pombe mitochondria (Fig. 3C). At first glance only intermediate length protein could be observed, but upon closer examination of the autoradiographs small amounts of mature length protein were detected. To show this more clearly, an altered S. cerevisiae iron-sulfur protein in ...
as a PDF - PubAg
... one trypsin-like cDNA, L1GtP1, were cloned from salivary glands and gut, respectively. Putative trypsin precursors from all cloned cDNAs contained a signal peptide, activation peptide, and conserved N-termini (IVGG). Other structural features included His, Asp, and Ser residues for the catalytic ami ...
... one trypsin-like cDNA, L1GtP1, were cloned from salivary glands and gut, respectively. Putative trypsin precursors from all cloned cDNAs contained a signal peptide, activation peptide, and conserved N-termini (IVGG). Other structural features included His, Asp, and Ser residues for the catalytic ami ...
attachment of amino acids to tRNA
... molecules in cell (~40). Each tRNA molecule is attached to a specific amino acids (20) and each recognizes a particular codon, or codons (61), in the mRNA. All tRNAs end with the sequence 5’CCA-3’ at the 3’ end, where the aminoacyl tRNA synthetase adds the amino acid. ...
... molecules in cell (~40). Each tRNA molecule is attached to a specific amino acids (20) and each recognizes a particular codon, or codons (61), in the mRNA. All tRNAs end with the sequence 5’CCA-3’ at the 3’ end, where the aminoacyl tRNA synthetase adds the amino acid. ...
Enzyme Mechanisms - Illinois Institute of Technology
... Specific loci in DNA targeted for alteration Typically involves excision, addition of altered bases, and religation Can be accomplished even in eukaryotic cell systems Many biochemical systems can be systematically probed this way: ...
... Specific loci in DNA targeted for alteration Typically involves excision, addition of altered bases, and religation Can be accomplished even in eukaryotic cell systems Many biochemical systems can be systematically probed this way: ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... component (20%) in the eye lens of the gecko Lepidodactylus lugubris. Limited peptide sequence analysis earlier revealed that it belongs to the aldo-keto reductase superfamily, as does the frog lens -crystallin. We have now determined the complete cDNA sequence of Bcrystallin and established that ...
... component (20%) in the eye lens of the gecko Lepidodactylus lugubris. Limited peptide sequence analysis earlier revealed that it belongs to the aldo-keto reductase superfamily, as does the frog lens -crystallin. We have now determined the complete cDNA sequence of Bcrystallin and established that ...
PDF
... has a predominantly S = 3 / 2 ground state which gives rise to an approx, g = 5 EPR signal [12]. This unusual property is thought to be related to the partial non-cysteinyl coordination to the cluster, a property not shared by the ferredoxin of the hyperthermophile T. litoralis (see Fig. 3). ...
... has a predominantly S = 3 / 2 ground state which gives rise to an approx, g = 5 EPR signal [12]. This unusual property is thought to be related to the partial non-cysteinyl coordination to the cluster, a property not shared by the ferredoxin of the hyperthermophile T. litoralis (see Fig. 3). ...
The Role in Translation of Editing and Multi
... the aaRS activities of the MSC were enriched in isolated T. kodakarensis polysome fractions. These in vivo data indicate that components of the archaeal protein synthesis machinery associate into macromolecular assemblies and could potentially increase translation efficiency by limiting substrate di ...
... the aaRS activities of the MSC were enriched in isolated T. kodakarensis polysome fractions. These in vivo data indicate that components of the archaeal protein synthesis machinery associate into macromolecular assemblies and could potentially increase translation efficiency by limiting substrate di ...
Multiple Functions of the New Cytokine
... Thymic stromal lymphopoietin (TSLP) was first identified in the culture supernatant of a murine thymic stromal cell line and was shown to support B-cell growth and development [1]. The human homologue of TSLP was cloned and characterized in 2001 and showed only 43% amino acid sequence identity with ...
... Thymic stromal lymphopoietin (TSLP) was first identified in the culture supernatant of a murine thymic stromal cell line and was shown to support B-cell growth and development [1]. The human homologue of TSLP was cloned and characterized in 2001 and showed only 43% amino acid sequence identity with ...