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Significance of Intestinal Digestion of Dietary Protein
Significance of Intestinal Digestion of Dietary Protein

... compared these values with true intestinal digestibility of proteins according to tabular values for 29 feeds. Tabular values were found to be generally higher than those estimated using the MBT (89.8 vs 84.4%). With both evaluation methods, feeds of plant origin seemed more digestible than those of ...
2006 Program
2006 Program

... “Staphylococcus aureus metabolism in a biofilm” Kendall A. Bryant, Steven H. Hinrichs, and Paul D. Fey “The characterization of the macromolecular synthesis operon of Staphylococcus epidermidis” Michael E. Olson, Luke D. Handke, Shawn R. Slater, and Paul D. Fey “The role of icaR in the regulation of ...
The Early Interaction of the Outer Membrane Protein PhoE with
The Early Interaction of the Outer Membrane Protein PhoE with

... action with trigger factor, a cytoplasmic chaperone. Using a cross-linking approach, the latter protein was found to interact with nascent PhoE chains as short as 57 amino acids (1–3). Subsequently, at a late co-translational or early post-translational stage the precursor protein interacts with Sec ...
Minimalist Active-Site Redesign: Teaching Old Enzymes New Tricks
Minimalist Active-Site Redesign: Teaching Old Enzymes New Tricks

Case Study: BPTI
Case Study: BPTI

Purification and Characterization of Two Thermostable Proteases
Purification and Characterization of Two Thermostable Proteases

... environment; on the other hand, the thermal stability characteristic of the protease from C. thermophilum makes the enzyme suitable for use in industry because of its tolerance to high temperature. The thermal stability of the proteases from C. thermophilum was significantly increased by Ca2+, simil ...
Protein-protein interactions: mechanisms and
Protein-protein interactions: mechanisms and

... The charge density varies from 0 to 12 charged groups per interface surface (Xu et al., 1997b). The distribution of the opposite charges in the interfaces of the contacting area showed that salt bridges across them are highly favourable (Drozdov-Tikhomirov et al., 2001; Xu et al., 1997a,b). The deso ...
The Genetic Code
The Genetic Code

bio98a_l10
bio98a_l10

... enough is available for proper cellular function. Therefore, enzymes often are highly regulated by binding small molecule regulators that can either decrease or increase activity. A classic example is in amino acid metabolism. Several enzymes are required to convert simple substrates into more compl ...
Amino Acid δ13C Analysis Shows Flexibility in the Routing of
Amino Acid δ13C Analysis Shows Flexibility in the Routing of

... use of resources and/or habitats. SIA is based on the premise that the isotopic composition of a consumer’s tissues originates from its food, but is offset by trophic-discrimination (enrichment) factors controlled by metabolic processes associated with the assimilation of nutrients and the biosynthe ...
Amino acid concentrations in fluids from the bovine oviduct and
Amino acid concentrations in fluids from the bovine oviduct and

... The results of individual amino acid concentrations and their relative abundance in bovine oviductal and uterine fluids recovered from slaughterhouse reproductive tracts are shown in Table 2. The non-essential amino acid group was the most abundant in OF and comprised 82% of the total amino acids me ...
Safety Assessment of Milk Proteins and Protein Derivatives as Used
Safety Assessment of Milk Proteins and Protein Derivatives as Used

pdf-1 - The Wolfson Centre for Applied Structural Biology
pdf-1 - The Wolfson Centre for Applied Structural Biology

... manufacture probes for detection of molecules of interest in a variety of research and diagnostic applications. No other current technology allows researchers to design and manufacture such highly specific molecular recognition tools. In addition to their high specificity, several important features ...
Are You suprised ?
Are You suprised ?

... The biochemical comparison of proteins is a technique used to determine evolutionary relationships among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids i ...
Relative Reactivity of Amino Acids with Chlorine
Relative Reactivity of Amino Acids with Chlorine

... The relative reactivity of chlorine with amino acids is an important determinant of the resulting chlorination products in systems where chlorine is the limiting reagent, for example, in the human gastrointestinal tract after consumption of chlorine-containing water, or during food preparation with ...
The Family of Berberine Bridge Enzyme-like
The Family of Berberine Bridge Enzyme-like

... AtBBE-like protein 13 was characterized by my dear colleague Barbara Steiner and was shown to possess the same function. AtBBE-like protein 15 is localized in the plant cell wall and catalyses the oxidation of one of the major cell wall components, the monolignols, to the corresponding aldehydes. Th ...
Translocation of proteins across the cell envelope of Gram
Translocation of proteins across the cell envelope of Gram

... 7.1. Competition between folding and degradation 7.2. Passage through the cell wall . . . . . . . . . . . . . ...
Protein relative abundance patterns associated with sucrose
Protein relative abundance patterns associated with sucrose

... Background: The etiology of dental caries is multifactorial, but frequent consumption of free sugars, notably sucrose, appears to be a major factor driving the supragingival microbiota in the direction of dysbiosis. Recent 16S rRNA-based studies indicated that caries-associated communities were less ...
Biochemistry 304 2014 Student Edition Amino Acid Metabolism
Biochemistry 304 2014 Student Edition Amino Acid Metabolism

... Distinguishing Protein Lifetimes The N-end Rule: N-terminal residues Asp, Arg, Leu, Lys & Phe half-life ~ 2-3 minutes Ala, Gly, Met, Ser Thr, & Val half-life > 20 hrs in eukaryotes (>10 prokaryotes) PEST proteins Proteins with segments rich in Pro, Glu, Ser, & Thr are rapidily degradedthese AA have ...
Landick R, Yanofsky C. 1987. Transcription
Landick R, Yanofsky C. 1987. Transcription

... rate of tRNATrp charging, and the overall rate of protein synthesis. Conditions that deplete the pool of charged tRNA Trp, such as tryptophan starvation or rapid protein synthesis, increase readthrough at the attenuator (17, 120). Similarly, all changes that reduce the efficiency of Trp codon transl ...
Neighbor-Dependent Ramachandran Probability Distributions of
Neighbor-Dependent Ramachandran Probability Distributions of

... Ramachandran distributions may also be affected by the identity or conformation of neighboring amino acids. In particular, it has long been known that residues that precede proline have quite different Ramachandran distributions [22], with significantly less density in the a and left-handed regions ...
Angiotensin I Converting Enzyme and the Changes
Angiotensin I Converting Enzyme and the Changes

... contained thiol groups. One of them was penicillamine, which is structurally related to captopril, as established much later. (With the present insight into the issues of ACE, derived mostly from hindsight and now knowing about captopril, it would have been better to do no other research but work on ...
emboj2009339-sup
emboj2009339-sup

... Peptides were covalently coupled via carboxyl groups to aminoaryl polyvinylidene difluoride membrane (Sequelon membranes), according to manufacturer’s instructions (Applied Biosystems, Foster City, CA). Briefly, the coupling procedure was initiated by spotting the sample to be coupled in 5μL aliquot ...
circular paper chromatography 95 - Journal of the Indian Institute of
circular paper chromatography 95 - Journal of the Indian Institute of

... A small circle of about 4 cm. diameter wa, drawn with a pencil from the centre of a circuIrrr filter-paper. The soh!tion (about 1-·8{"1.) containing mixture of amino ,\cids to be r.nalyscd, was applied along the circumference of the circle from the tip of a capillary lllbe. Usually maximum number of ...
C urrent and prospective applications of metal ion–protein
C urrent and prospective applications of metal ion–protein

... 2. Immobilized metal ion affinity chromatography: utilization of differences in protein–metal ion affinity ......................................... 2.1. Principles and procedures ........................................................................................................................ ...
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Ribosomally synthesized and post-translationally modified peptides

Ribosomally synthesized and post-translationally modified peptides (RiPPs), also known as ribosomal natural products, are a diverse class of natural products of ribosomal origin. Consisting of more than 20 sub-classes, RiPPs are produced by a variety of organisms, including prokaryotes, eukaryotes, and archaea, and they possess a wide range of biological functions.As a consequence of the falling cost of genome sequencing and the accompanying rise in available genomic data, scientific interest in RiPPs has increased in the last few decades. Because the chemical structures of RiPPs are more closely predictable from genomic data than are other natural products (e.g. alkaloids, terpenoids), their presence in sequenced organisms can, in theory, be identified rapidly. This makes RiPPs an attractive target of modern natural product discovery efforts.
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