Structure-based design of inhibitors of NS3 serine protease
... Asp:81 and Ser:139) is located between these two regions and is formed by a shallow solvent exposed pocket requiring many interaction points for binding of substrates or inhibitors [8–11]. Thus, the NS3 protease displays substrate specificity that requires relatively large peptides spanning the acti ...
... Asp:81 and Ser:139) is located between these two regions and is formed by a shallow solvent exposed pocket requiring many interaction points for binding of substrates or inhibitors [8–11]. Thus, the NS3 protease displays substrate specificity that requires relatively large peptides spanning the acti ...
Molecular Cloning of Dog Mast Cell Tryptase and a Related Protease
... Construction of a Mastocytoma cDNA Library. Total RNA was isolated from collagenase-disaggregated BR dog mastocytoma cells (Caughey et al., 1988a) using guanidine isothiocyanate (Chirgwin et al, 1979). Poly(A) RNA was then enriched by two passages on oligo(dT)*llulose (New England Biolabs) (Aviv & L ...
... Construction of a Mastocytoma cDNA Library. Total RNA was isolated from collagenase-disaggregated BR dog mastocytoma cells (Caughey et al., 1988a) using guanidine isothiocyanate (Chirgwin et al, 1979). Poly(A) RNA was then enriched by two passages on oligo(dT)*llulose (New England Biolabs) (Aviv & L ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine ...
... Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine ...
HIGHLY VISCOUS DOUGH FORMING PROPERTIES OF MARAMA PROTEIN
... deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for the structural stability of proteins (Mezger, 2006). A low tan δ value is often associated with a high degree of crosslinking (Tsiami, Bot, Agterof, & Groot, 1997; M ...
... deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for the structural stability of proteins (Mezger, 2006). A low tan δ value is often associated with a high degree of crosslinking (Tsiami, Bot, Agterof, & Groot, 1997; M ...
Modulation of the Oligomerization State of the Bovine F1
... fragment [IF1 (residues 44 – 84)] was described earlier (11). Overexpression and Purification of the Bovine IF1 and Related Proteins—The overexpression of IF1 and related proteins was carried out as described previously (11) except that the host cell was Escherichia coli C41 (16). After addition of ...
... fragment [IF1 (residues 44 – 84)] was described earlier (11). Overexpression and Purification of the Bovine IF1 and Related Proteins—The overexpression of IF1 and related proteins was carried out as described previously (11) except that the host cell was Escherichia coli C41 (16). After addition of ...
Extending the limits of paleodietary studies of humans with
... Serine, proline, and leucine, for example, have the smallest differences between the two plant groups, while alanine, threonine, and valine display the greatest differences. The data for serine suggest substantial isotopic enrichment of 13C in the photorespiratory pathway of C3 plants, which are refle ...
... Serine, proline, and leucine, for example, have the smallest differences between the two plant groups, while alanine, threonine, and valine display the greatest differences. The data for serine suggest substantial isotopic enrichment of 13C in the photorespiratory pathway of C3 plants, which are refle ...
Collagen Peptide - Collagen Products
... variations in size are observed. Gelatin is soluble in hot water but forms a gel when dissolved in cold water. "Collagen peptide" is prepared by decomposing gelatin into smaller sizes using proteinase. Collagen peptide does not form a gel and readily dissolves in cold water, even at relatively high ...
... variations in size are observed. Gelatin is soluble in hot water but forms a gel when dissolved in cold water. "Collagen peptide" is prepared by decomposing gelatin into smaller sizes using proteinase. Collagen peptide does not form a gel and readily dissolves in cold water, even at relatively high ...
PDF of original
... represent the first and second bases, respectively, in the triplet) correspond to the same amino acid; often XYA and XYG correspond to the same amino acid; sometimes XYG alone corresponds to an amino acid. For eight amino acids, U, C, A, or G may occupy the third position of synonym codons. Alternat ...
... represent the first and second bases, respectively, in the triplet) correspond to the same amino acid; often XYA and XYG correspond to the same amino acid; sometimes XYG alone corresponds to an amino acid. For eight amino acids, U, C, A, or G may occupy the third position of synonym codons. Alternat ...
Proteolytic processing in the secretory pathway of Aspergillus niger
... a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases, and none have been found in filamentous fungi so far. The serine proteases contain a catalytic triad, which is formed by a serine, a histidine and ...
... a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases, and none have been found in filamentous fungi so far. The serine proteases contain a catalytic triad, which is formed by a serine, a histidine and ...
Intrinsically Disordered Protein - Center for Data Analytics and
... calcineurin helix within a region of disorder is essential for enabling calmodulin to surround its target upon binding. 5. Calsequestrin regulates calcium levels in the sarcoplasmic reticulum by binding about 50 ions/molecule. Disordered polyanion tails at the carboxy terminus bind many of these cal ...
... calcineurin helix within a region of disorder is essential for enabling calmodulin to surround its target upon binding. 5. Calsequestrin regulates calcium levels in the sarcoplasmic reticulum by binding about 50 ions/molecule. Disordered polyanion tails at the carboxy terminus bind many of these cal ...
5-1 Necleotide Metabolism (purine)
... (a transition state analog) shows promise in combination with Ara-C (Vidarabine) ...
... (a transition state analog) shows promise in combination with Ara-C (Vidarabine) ...
Presentation @3:30pm - Bioinformatics at School of Informatics
... •Why ISPTM? •Feautures of ISPTM •Targets for Future •References •Acknowledgements Translation is the process of synthesizing the peptide chain of amino acids specified by the nucleotide sequence on the mRNA. ...
... •Why ISPTM? •Feautures of ISPTM •Targets for Future •References •Acknowledgements Translation is the process of synthesizing the peptide chain of amino acids specified by the nucleotide sequence on the mRNA. ...
Prokaryotic features of a nucleus
... sequences and relatively similar to the GAPDHs of thermophilic bacteria. These results are compatible with the hypothesis that the nuclear gene for subunit A of chloroplast GAPDH is of prokaryotic origin. They are in puzzling contrast with a previous publication demonstrating that Escherichia coli G ...
... sequences and relatively similar to the GAPDHs of thermophilic bacteria. These results are compatible with the hypothesis that the nuclear gene for subunit A of chloroplast GAPDH is of prokaryotic origin. They are in puzzling contrast with a previous publication demonstrating that Escherichia coli G ...
Predicting the sidechain dihedral angle distributions
... steric repulsive interactions to the side-chain dihedral angle distributions and build-in the observed side-chain dihedral angle distributions that we seek to predict. In this manuscript, we pose the key question: To what extent, do steric interactions plus stereochemical constraints determine the s ...
... steric repulsive interactions to the side-chain dihedral angle distributions and build-in the observed side-chain dihedral angle distributions that we seek to predict. In this manuscript, we pose the key question: To what extent, do steric interactions plus stereochemical constraints determine the s ...
from Scolopendra subspinipes mutilans
... previous studies [5, 6]. The venoms also play significant biologic roles in the synthesis of proteins, peptides and enzymes. In a recent study, centipede venoms that contained about 500 proteins and peptides were involved in platelet aggregating activity, phospholipaseA2 activity, anticoagulant acti ...
... previous studies [5, 6]. The venoms also play significant biologic roles in the synthesis of proteins, peptides and enzymes. In a recent study, centipede venoms that contained about 500 proteins and peptides were involved in platelet aggregating activity, phospholipaseA2 activity, anticoagulant acti ...
Standard for the presentation of nucleotide and amino acid
... “amino acids” are those L-amino acids commonly found in naturally occurring proteins and are listed in Appendix 2, Table 3. Those amino acid sequences containing at least one D-amino acid are not intended to be embraced by this definition. Any amino acid sequence that contains post-translationally m ...
... “amino acids” are those L-amino acids commonly found in naturally occurring proteins and are listed in Appendix 2, Table 3. Those amino acid sequences containing at least one D-amino acid are not intended to be embraced by this definition. Any amino acid sequence that contains post-translationally m ...
Protein Structure Hierarchy
... extremes can be classified as pacifists and activists. The pacifists view turns as a weak link in the polypeptide chain, allowing the other secondary structures (helix and sheet) to determine the conformational outcome. The activists, (encouraged by the recent experimental finding of "turn-like" str ...
... extremes can be classified as pacifists and activists. The pacifists view turns as a weak link in the polypeptide chain, allowing the other secondary structures (helix and sheet) to determine the conformational outcome. The activists, (encouraged by the recent experimental finding of "turn-like" str ...
Amino Acid Degradation
... a similar manner that pyruvate dehydrogenase is phosphorylated and inactivated. The intake of dietary branched amino acids activates a phosphatase which activates this enzyme. A genetic deficiency in the branched chain α-ketoacid dehydrogenase enzyme is called maple syrup urine disease. The deficien ...
... a similar manner that pyruvate dehydrogenase is phosphorylated and inactivated. The intake of dietary branched amino acids activates a phosphatase which activates this enzyme. A genetic deficiency in the branched chain α-ketoacid dehydrogenase enzyme is called maple syrup urine disease. The deficien ...
Structure based hypothesis of a mitochondrial
... of Ser-195 to glycine in mtRF1, most likely to accommodate the inserted threonine side chain. It should be noted that this is also one of the identified critical changes between mtRF1 and mtRF1a that we identified. The altered loop conformation that results from the RTinsert seems to completely bloc ...
... of Ser-195 to glycine in mtRF1, most likely to accommodate the inserted threonine side chain. It should be noted that this is also one of the identified critical changes between mtRF1 and mtRF1a that we identified. The altered loop conformation that results from the RTinsert seems to completely bloc ...
New peptide and gene coding for same
... al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisti ...
... al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisti ...
Corticotropin-Releasing Factor Receptor Antagonists: Potential
... using both receptor autoradiography and in situ hybridization studies. Using these techniques, CRF binding sites and CRF receptor mRNA have been localized in anatomically and physiologically relevant areas. For example, the highest density of CRF1 receptors exists in the pituitary gland, where CRF1 ...
... using both receptor autoradiography and in situ hybridization studies. Using these techniques, CRF binding sites and CRF receptor mRNA have been localized in anatomically and physiologically relevant areas. For example, the highest density of CRF1 receptors exists in the pituitary gland, where CRF1 ...
Geometrical and Sequence Characteristics of
... by the size of the protein (Creighton, 1993). Thus one might expect a correlation between the helix length and the size of the protein as defined by the number of amino acid residues in the protein. In our database, we do not observe any such correlation. For example, a small 56-residue polypeptide ...
... by the size of the protein (Creighton, 1993). Thus one might expect a correlation between the helix length and the size of the protein as defined by the number of amino acid residues in the protein. In our database, we do not observe any such correlation. For example, a small 56-residue polypeptide ...
DESIGNING OF A POTENT ANALOG AGAINST DRUG RESISTANT HIV-1 PROTEASE:... STUDY Research Article
... pocket is measured by ProtScale tool. The FASTA sequences of the protease enzyme (1HPV) were pasted in the box provided followed by their submission.The tool plots the hydrophobic values of each amino acids present in the sequence in the form of graph as well as individual amino acids also. Hydropho ...
... pocket is measured by ProtScale tool. The FASTA sequences of the protease enzyme (1HPV) were pasted in the box provided followed by their submission.The tool plots the hydrophobic values of each amino acids present in the sequence in the form of graph as well as individual amino acids also. Hydropho ...