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台中班 2012 暑期生化小考
單選題 (每題 2%, 70%)
1. Glutathione 是由下列那種序列排列而成？
(A) Glu-Cys-Ala
(B) Gln-Cys-Ala
(C) Glu-Cys-Gly (D) Gln-Cys-Gly
2. It is often convenient to assay an enzyme reaction by coupling it with another enzyme to form a product
that is easily quantified. An example is the assay of pyruvate kinase by coupling it with lactate
dehydrogenase.
Phosphoenolpyruvate + ADP + H+ → pyruvate + ATP
Pyruvate + NADH + H+ → lactate + NAD+
In relation to this assay procedure, which one of the following statements is correct？
(A)Of the two enzymes, pyruvate kinase should be in excess.
(B)The reaction could be followed by measuring the increase in absorbance at 340 nm.
(C)The reaction could be followed by measuring the decrease in absorbance at 260 nm.
(D)The total change in NADH concentration provides a measure of pyruvate kinase activity.
3. 以下敘述何者是正確的?
(A) Km 代表酵素跟受質之間的親和力
酶
(B) Km 的單位是濃度單位
(D)絲胺酸蛋白酶(Ser protease)是由 Asp 去進行共價催化
(C) M-M 方程式也適用於異位
(E) ATCase 是絲胺酸蛋白酶的代表例
之一
4.最好的受質(substrate)是下列所代表之值要高：
(A) Vmax/Km (B) Km/Vmax
(C) 1/Vmax
(D) 1/Km
5.有一種藥物(I)，對某酵素(enzyme)(E)活性之抑制作用(inhibition)表現如下圖形(Lineweaver-Burk
plot)，以下何者是錯誤的?
1
V
+I
I
1
[ S]
(A) I 對 E 是 irreversible inhibition
(B) I 對 E 是 competitive inhibition
(C)基質(S)之 apparent Michaelis constant (Km)增加
(D) S 之濃度比 I 之濃度相當大時，I 對 E 不具抑制作用
6. Allosteric enzymes, which typically control the rate of throughput in a metabolic pathway：
(A) generally show a hyperbolic dependence of initial rate on substrate concentration.
(B) are always oligomeric.
(C) alter the equilibrium position of the reaction they catalyse.
(D) catalyse the final reaction in a metabolic pathway.
7. Serum glutamate-oxaloacetate transaminase (GOT)：
(A) requires biotin as a cofactor.
(B) activity is raised after myocardial infarction.
not be raised after liver damage.
(D) is a source of ATP in erythrocytes.
(C) activity would
8. 關於核黃素(Riboflavin)，以下何者是錯誤的？
(A)為一般所熟知的 Vitamin B2
(B)其 Coenzyme form 為 NAD＋
(C)為一水溶性維生素
(D)缺乏
時會造成口角炎
9. What vitamins are the precursors of CoA and FAD used in the TCA cycle？
(A) pantothenic acid and thiamin
(B) thiamin and niacin
(C) niacin and riboflavin
(D) riboflavin
and pantothenic acid
10. Which one of the following soluble vitamins is a portion of Acetyl-CoA？
(A) Riboflavin
(B) Niacin
(C) Thiamin
(D) Pantothenic acid
11. Folate 含有下列那一個胺基酸？
(A) glycine
(B) tyrosine
(C) tryptophan
(D) glutamate
(E) methionine
12. In human, ascorbic acid can be derived from
(A) Pentose phosphate pathway
(B) Uronic acid pathway
(C) Glucogenic amino acids
(D) None
of the mentioned
13. 以下何者”不是”原致癌基因(proto-oncogenes)被活化成致癌基因(oncogenes)的機制
(A) mutation
(B) insertion
(C) chromosome translocation
(D) gene amplification
(D) none of the
above
14. Choose the enzyme that can not be used for 32P-labeling a DNA fragment
(A) T4 DNA polymerase
(B) T4 DNA ligase
(C) T4 polynucleotide kinase
(D) Klenow fragment
15.人類基因體計畫的主要主持人是
(A) Watson and Crick
(B)Fischer and Krebs
(C)Collins and Venter
(D)Sanger and Gilbert
16.Uease 含有何種礦物質元素?
(A) Fe
(B) Ni
(C) Cu
(D) Zn
(E) Mn
17. 一分子 stearic acid 完全氧化需經：
(A) 9 次 β-氧化作用，產生 8 個 acetyl-CoA
(B) 8 次 β-氧化作用，產生 9 個 acetyl-CoA
(C) 9 次 β-氧化作用，產生 9 個 acetyl-CoA
(D) 8 次 β-氧化作用，產生 8 個 acetyl-CoA
18.使 fatty acyl-CoA 進入粒線體的攜帶者(carrier)為
(A) hemoglobin
(B) insulin
(C) carnitine
(D) albumin
19.下列有關膽固醇(cholesterol)的敘述，何者正確?
(A)和性激素的前驅物(precursor)無關
途徑的中間產物之一
(B)是維生素 E 的前驅物(precursor)
(C)squalene 為其生合成
(D)轉化成膽酸(bile acid)時，需要維生素 D 參與反應
20.在脂肪代謝產物中何者不為酮體(ketone bodies)
(A) acetoacetate
(B) D-β-hydroxybutyrate
(C) acetone
(D) L-β-hydroxybutyrate
21.脂肪酸中 linoleic acid 內，雙鍵的位置在
(A) C9 與 C10；及 C11 與 C12 之間
與 C12 之間
(B) C9 與 C10；及 C12 與 C13 之間
(C) C8 與 C9；及 C11
(D) C10 與 C11；及 C13 與 C14 之間
22. D-amino acid 不存在於：
(A)生物體之蛋白質
(B)抗生素
(C)細菌細胞壁
(D) actinomycin D
23. The radioimmunossay (RIA) is based on competition of unlabeled and radiolabeled:
(A) antibodies for binding to a hormone.
binding to a receptor.
(B) antibodies for binding to a receptor.
(D) hormone for binding to an antibody.
(C) hormone for
(E) receptor for binding to a hormone.
24. One distinction between peptide and steroid hormones is that peptide hormones:
(A) act through nonspecific receptors, whereas steroid hormones act through specific receptors.
(B) are generally water-insoluble, whereas steroid hormones are water soluble.
(C) are more stable than steroid hormones.
(D) bind to cell surface receptors, whereas steroid hormones bind to nuclear receptors.
(E) bind to their receptors with high affinity, whereas steroid hormones bind with low affinity.
25. Insulin is an example of a(n) ____________ hormone.
(A) catecholamine
(B) eicosanoid
(C) paracrine
(D) peptide
(E) steroid
26. The maturation of insulin from its precursor (preproinsulin) involves:
(A) acetylation.
(B) oxidation.
(C) phosphorylation.
(D) proteolysis.
(E) reduction.
27. Epinephrine is an example of a(n) ____________ hormone.
(A) catecholamine
(B) eicosanoid
(C) paracrine
(D) peptide
(E) steroid
28. An example of an eicosanoid hormone is:
(A) epinephrine.
(B) retinoic acid.
(C) testosterone.
(D) thromboxane.
(E) thyroxine.
29. Which one of the following statements is true?
(A) The brain prefers glucose as an energy source, but can use ketone bodies.
(B) Muscle cannot use fatty acids as an energy source.
(C) In a well-fed human, about equal amounts of energy are stored as glycogen and as triacylglycerol.
(D) Fatty acids cannot be used as an energy source in humans because humans lack the enzymes of the
glyoxylate cycle.
(E) Amino acids are a preferable energy source over fatty acids.
30. When blood glucose is abnormally high, the pancreas releases:
(A) epinephrine.
(B) glucagon.
(C) glucose.
(D) insulin.
(E) trypsin.
31. An elevated insulin level in the blood:
(A) inhibits glucose uptake by the liver.
(B) inhibits glycogen synthesis in the liver and muscle.
results from a below-normal blood glucose level.
(D) stimulates glycogen breakdown in liver.
stimulates synthesis of fatty acids and triacylglycerols in the liver.
32. The “largest” energy store in a well-nourished human is:
(A) ATP in all tissues.
(B) blood glucose.
(C) liver glycogen.
triacylglycerols in adipose tissue.
33. Elevated epinephrine levels do not normally stimulate:
(D) muscle glycogen.
(E)
(C)
(E)
(A) fatty acid mobilization in adipose tissue.
muscle.
(D) glycogen synthesis in liver.
(B) gluconeogenesis in liver.
(C) glycogen breakdown in
(E) glycolysis in muscle.
34. vit K involves in
(A) reduction
(B) ligation
(C) carboxylation
(D) peroxidatio
(E) nitrogen fixation
35. chlorophyll contains
(A) Cu2+
(B) Fe2+
(C) Zn2+
(D) Ca2+
(E) Mg2+
問答題 (30%)
1. 下列蛋白質經過分子篩選管柱時，其沖提之順序為何。(2%)
(A) hemoglobin (B) insulin
(C) myoglobin
(D) immunoglobulin G
2. 生長因子(growth factors)的作用方式可以有：endocrine、autocrine 或________。 (2%)
3. 在基因工程中, 所使用的 Southern, Northern, 與 Western blottings 其原理及分析之主要對象各為
何？ (6%)
Southern
Northern
Western
探針
分析對象
4. 試說明調控體內酵素(enzyme)活性的方法。(2%)
5. 試寫出酵素催化作用之速率方程式 (2%)
6.可逆性的抑制作用(reversible inhibition)有哪三種? (3%)
7.致癌基因根據其產物來分，可分成哪五類? (5%)
8.Ser protease family 的活性位含有哪三種對催化反應進行極為重要的胺基酸?
9.口腔內有哪兩種消化相關的酵素? (2%)
10. 羧基化作用(carboxylation)的輔因子為哪種維生素? (1%)
(3%)
台中班 2012 暑期生化小考
單選題 (每題 2%, 70%)
1. (C)
6. (B)
11.(D)
16.(B)
21.(A)
26.(D)
31.(E)
2. (D)
7. (B)
12.(D)
17. (B)
22.(A)
27. (A)
32. (E)
3. (B)
8. (B)
13.(D)
18.(C)
23.(D)
28. (D)
33.(D)
4. (A)
9. (D)
14. (C)
19.(C)
24.(D)
29. (A)
34. (C)
5. (A)
10. (D)
15.(C)
20.(D)
25.(D)
30. (D)
35. (E)
問答題 (30%)
1. (D) (A) (C) (B)
2. paracrine
3.
Southern
Northern
Western
探針
cDNA
cDNA
抗體(antibody)
分析對象
DNA
RNA
蛋白質(protein)
4. 共價修飾(covalent modification)
5. V = Vmax [S]/(Km+ [S])
6. (1) competitive inhibition
異位調控(allosteric regulation)
(註: 分子為 Vmax [S]
(2) noncompetitive inhibition
分母為 Km+ [S] )
(3) uncompetitive inhibition
7. (1)生長因子(growth factor) (2)接受體(receptor) (3)G 蛋白(GTP-binding protein)
(5) DNA 結合蛋白 (DNA-binding protein)
8. Ser, His, Asp
或寫全名皆可(serine, histidine, aspartate)
9.唾液澱粉酶(amylase) & 脂肪分解酶(lipase)
10. 生物素(biotin)
(4)蛋白質激酶