Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Jatropha genotyping In Gh Pu QR In Gh Pu QR 13 primer pairs
Document related concepts
DNA damage theory of aging wikipedia , lookup
DNA paternity testing wikipedia , lookup
DNA profiling wikipedia , lookup
DNA vaccination wikipedia , lookup
Genetic testing wikipedia , lookup
Genetically modified food wikipedia , lookup
Heritability of IQ wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Point mutation wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Genomic library wikipedia , lookup
Epigenomics wikipedia , lookup
Public health genomics wikipedia , lookup
Molecular cloning wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Human genetic variation wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Non-coding DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Genome (book) wikipedia , lookup
SNP genotyping wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Microsatellite wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Genome editing wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Quantitative trait locus wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Designer baby wikipedia , lookup
Helitron (biology) wikipedia , lookup
Genetic engineering wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Transcript
Getting Biodiesel From Jathropa The jatropha seeds, commonly known as the physic nuts, contain up to 40% oil. The jatropha oil can be used directly as biofuel or can be converted into biodiesel for a more efficient performance. When raw or mixed jatropha oil is directly used in an automobile or even plane engines Jatropha genotyping In Gh Pu QR In Gh Pu QR • 13 primer pairs selected for use in further studies • These reveal 69/453 polymorphic bands (15.2%) • Results indicate very little variation between accessions from India, Ghana, Tanzania & Madagascar Considerations for using DNA markers in plant breeding • Technical methodology – simple or complicated? • • • • • Reliability Degree of polymorphism DNA quality and quantity required Cost** Available resources – Equipment, technical expertise Genetic Markers • A gene or DNA sequence having a known location on a chromosome and associated with a particular gene or trait • Advantages of genetic markers: – Profile of genetic markers will define a genotype – – – – Uniform at all tissues at all ages of the organism allows early detection developmentally stable and not affected by the environment highly specific pattern can be developed for each individual • Utilities: – For biodiversity study – To choose right parents for cross pollination – Marker assisted breeding – To differentiate plant varieties – Quality control of plantation RFLP Overview of ‘marker genotyping’ (1) LEAF TISSUE SAMPLING (2) DNA EXTRACTION (3) PCR (4) GEL ELECTROPHORESIS (5) MARKER ANALYSIS
