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Laboratory Profile Principal Investigator: Nir Friedman Systems Biology of Transcription and Chromatin Location: Silberman Wing 2, 3rd. floor Room 2-313 Communication: E.Mail: [email protected] Phone: 02-6584295 Web: www.cs.huji.ac.il/~nir Brief Description of Research: We aim to decipher the complex pathways that control transcription and how cells maintain their transcriptional state via chromatin. These are central basic questions for many biological systems, including cancer and other human diseases. We use yeast as a model organism, since it provides for powerful genetics and experimental tools, and yet shares many of the basic regulatory and chromatin mechanisms with all eukaryotes. Our main tool is using genetic screens to characterize mutations that impact the induction of stress-response genes. These screens are based on fluorescent tagging of target genes and measurement of protein induction by fluorescence microscopy/FACS. These screens are complemented by measurements of mRNA levels, chromatin modifications, and protein localization to characterize the interactions between cellular machineries involved in this regulation and understand their dynamics. Techniques used include: Yeast genetics, time-lapse microscopy, flowcytometry, and functional genomics assays (mRNA-seq, ChIP-seq). Automation (liquid handling robotics). Advanced quantitative data analysis. Stochastic models. Proposed Research Projects: Epigenetic memory: Finding genes involved in cross-generational stress adaptation. Dissecting pathways that regulate nuclear localization of stress-activated transcription factors.