Download Assessment of Toxicity of Nanoparticles in Vero and MDCK Cell

SBBq
XL Annual Meeting of Brazilian Biochemistry and Molecular Biology Society
th
rd
Foz do Iguaçu, PR, Brazil, April 30 to May 3 , 2011
Assessment of Toxicity of Nanoparticles in Vero and MDCK Cell Lineages.
Silva, A.H.1, Mattei, B.1, Zanetti-Ramos, B.G.1, Creczynski-Pasa, T.B.1,
1
Departamento de Ciências Farmacêuticas, UFSC, Florianópolis/SC, Brazil.
There is an increase in the interest in evaluation of the cytotoxicity induced by
nanostructured systems, because the use of such technology may affect the health.
The objective of this work was to evaluate the toxicity of lipid and polymeric
nanoparticles (NPs) in cell culture. Lipid nanoparticles (F1, F2, F3, F4, F5, F7 and F8)
and polymeric (F9) NPs were added in growing concentrations in the cell culture of Vero
and MDCK cell lines. The determination of cytotoxicity was performed using MTT and
Neutral Red methods. NPs F1, F2, F4 and F5 presented cytotoxicity to Vero cell
lineage, and NPs F4 and F5 to MDCK cells. The generation of reactive oxygen species
was detected fluorometrically, using dichlorofluorescein as a probe. NPs F4, F5 and F8
increased the generation of ROS in both cell lines. The mitochondrial membrane
potential was also evaluated by fluorometric method, using the probe JC-1. The F5 NPs
decreased the mitochondrial membrane potential. The results indicate that the
nanoparticles F1, F2, F4 and F5 were cytotoxic, while only the NPs F4, F5 and F8
increased the oxidative stress, which can induce cell death by necrosis or apoptosis.
The NP F5 also seems to lead a decrease in the mitochondrial membrane potential,
which is a key point in the cell death cascade. The other NPs did not influence cell
viability or cell metabolism in the lineages tested. We are further investigating the kind
of cell death that the cytotoxic NPs are inducing.
Keywords: Toxicicity, nanoparticles, cell lineages.
Supported by: CAPES/CNPQ/FAPESC.
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