Download Nanomaterials for Live Cell Tracking (2006

Nanomaterials for Live Cell Tracking
PI: Greg Boebinger, National High Magnetic Field Laboratory
Florida State University, University of Florida, Los Alamos National Laboratory
NSF Award Number: DMR-0084173
To increase the sensitivity and
resolution of magnetic resonance and optical
imaging of biological tissue, scientists at the
National High Magnetic Field Laboratory (NHMFL)
and the Advanced Magnetic Resonance Imaging
and Spectroscopy Facility developed multimodal
nanoparticles (NPs) that generate high optical
contrast and large magnetic resonance response.
Monodisperse NPs of many compositions have
been developed, each coated with an organic layer
to be compatible with living cells. These include
semiconductor quantum dots, super-paramagnetic
iron oxide NPs, magnetic NPs, dye-doped NPs,
and gadolinium (Gd) NPs. This interdisciplinary
research takes advantage of the NHMFL’s unique
high-field magnets through development of
contrast mechanisms that actually increase with
magnetic fields as high as 21 teslas (900 MHz).
At left are living cells labeled with Gd nanoparticles, each
containing a semiconductor quantum dot engineered to
provide very high contrast in large magnetic fields. The
image at right, taken at 750MHz, illustrates live cell
tracking in a neonatal mouse brain. Pink indicates
nanoparticle-labelled neuroprogenitor cells and models
damage resulting from hypoxia.
Grant, S.C.; et.al., “MR Microscopy of Multipotent Astrocytic Stem Cells Labeled with Multimodal Qdots
Applied to a Neonatal Murine Model of Hypoxic Ischemic Encephalopathy,”
International Society for Magnetic Resonance in Medicine Meeting, Seattle, WA., p. 1880 (May 2006).
Santra, S.; Dutta, D.; Walter, G.A. and Moudgil, B.M., “Fluorescent nanoparticle probes for cancer imaging,”
Technology for Cancer Research Treatment, 4 (6), 593-602 (2005).
Sharma, P.; Brown, S.; Walter, G.; Santra, S. and Moudgil, B., “Nanoparticles for bioimaging,”
Advanced Colloid Interface Science (2006).