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Nanomaterials for Live Cell Tracking PI: Greg Boebinger, National High Magnetic Field Laboratory Florida State University, University of Florida, Los Alamos National Laboratory NSF Award Number: DMR-0084173 To increase the sensitivity and resolution of magnetic resonance and optical imaging of biological tissue, scientists at the National High Magnetic Field Laboratory (NHMFL) and the Advanced Magnetic Resonance Imaging and Spectroscopy Facility developed multimodal nanoparticles (NPs) that generate high optical contrast and large magnetic resonance response. Monodisperse NPs of many compositions have been developed, each coated with an organic layer to be compatible with living cells. These include semiconductor quantum dots, super-paramagnetic iron oxide NPs, magnetic NPs, dye-doped NPs, and gadolinium (Gd) NPs. This interdisciplinary research takes advantage of the NHMFL’s unique high-field magnets through development of contrast mechanisms that actually increase with magnetic fields as high as 21 teslas (900 MHz). At left are living cells labeled with Gd nanoparticles, each containing a semiconductor quantum dot engineered to provide very high contrast in large magnetic fields. The image at right, taken at 750MHz, illustrates live cell tracking in a neonatal mouse brain. Pink indicates nanoparticle-labelled neuroprogenitor cells and models damage resulting from hypoxia. Grant, S.C.; et.al., “MR Microscopy of Multipotent Astrocytic Stem Cells Labeled with Multimodal Qdots Applied to a Neonatal Murine Model of Hypoxic Ischemic Encephalopathy,” International Society for Magnetic Resonance in Medicine Meeting, Seattle, WA., p. 1880 (May 2006). Santra, S.; Dutta, D.; Walter, G.A. and Moudgil, B.M., “Fluorescent nanoparticle probes for cancer imaging,” Technology for Cancer Research Treatment, 4 (6), 593-602 (2005). Sharma, P.; Brown, S.; Walter, G.; Santra, S. and Moudgil, B., “Nanoparticles for bioimaging,” Advanced Colloid Interface Science (2006).