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Chapter 7 A view of the cell 1 The Cell Theory • Microscope invented by Anton van Leeuwenhoek • First person to see a cell (in cork) Robert Hooke • Matthias Schleiden found that all plants have similar structures • Theodore Schwann found the same situation with animals • Hence, the cell theory was born 2 The Light Microscope • Uses light and lenses • The Simple light Microscope used one lens and natural light (Leeuwenhoek) • The Compound light microscope: Uses multiple lenses – Magnifies up to 1500 times The Electron Microscope • • • • Invented in the 1940s Uses a beam of electrons Magnifies up to 500,000 times Two Kinds: – Scanning electron microscope (SEM): Scans the surface of cells. – Transmission electron microscope (TEM): Allows for study of structures inside cells. The Electron Microscope SEM TEM • Cell theory –All organisms are composed of one or more cells –The cell is the basic unit of organization of organisms –All cells come from pre-existing cells 6 Two Basic Cell Types • Prokaryotes: Cells lacking internal membrane-bound structures • Eukaryotes: Cells containing internal membrane-bound structures – The membrane-bound structures are called organelles – Contains a nucleus: organelle that manages cellular function. First observed by Robert Brown. Rudolf Virchow concluded that it was responsible for cell division. 8 • Organelles – membrane-bound structures within eukaryotic cells – Each organelle has a specific function for cell survival (ie. Nucleus) 9 7.2 The Plasma Membrane 5/3/2017 10 • Plasma Membrane – the boundary between the cell and its environment – Responsible for allowing nutrients into and out of the cell – Allows waste and other products to leave the cell – Maintains homeostasis • Water freely enters the cell through the membrane 11 • Certain particles (Na+ ions & Ca+ ions) must be allowed in the cell only at certain times, in certain amounts, and through certain channels • Selective Permeability keeps a proper balance by allowing only certain molecules to cross the membrane • Homeostasis – the process of maintaining the cells environment 12 Structure of the Plasma Membrane • Composed of a phospholipid bilayer. – A Lipid with a phosphate group attached – Has only 2 fatty acid tails – Forms a sandwich • The phosphate group forms the polar head • The fatty acid tails form the nonpolar tail Plasma Membrane Structure 14 Phospholipids • Lipids with a phosphate group attached • Hydrophobic tails inside and hydrophilic heads on outside • The bilayer is a barrier that is water-soluble at the outer surface and water-insoluble in the middle 15 • Water-soluble molecules will not easily move through the membrane; they are stopped by the waterinsoluble middle • The polar phosphate group allows the cell membrane to interact with its watery environment 16 5/3/2017 17 Fluid Mosaic Model 5/3/2017 18 Fluid Mosaic Model • The membrane is fluid: It is flexible and phospholipids can move in the membrane like water in a lake. • The membrane is mosaic: There are proteins embedded in the membrane that also move (like boats in the lake) Components of Membrane • Cholesterol – helps stabilize the phospholipids – Prevents the fatty acid chains of the phospholipids from sticking together 20 • Transport Proteins – span the entire membrane, creates a selectively permeable membrane – Regulates which molecules enter and leave a cell 21 Other components • Other Proteins and carbohydrates on the external surface: Helps with identification. • Proteins on internal surface: Provides flexibility by attaching the plasma membrane to the cell’s internal structure. 7.3 Eukaryotic Cell Structure 23 Cellular Boundaries • Plasma membrane surrounds the cell • In plants, fungi, most bacteria and some protists, the cell wall surrounds the plasma membrane – Fairly rigid – Provides support and protection – Made up of the carbohydrate cellulose – Has pores to allow molecules through Nucleus and cell control • The Nucleus is the leader of the cells – Gives directions for the making of proteins • The master set of directions is in chromatin • During cell division, chromatin condenses to form chromosomes. • Chromatin – strands of genetic material, DNA – Condenses to form chromosomes 26 Nucleus and Cell Control • Inside the nucleus there is also the nucleolus – Makes ribosomes • Ribosomes – Assemble enzymes and proteins – Get instructions from DNA – Leave nucleus and enter cytoplasm in order to make proteins Nucleolus 28 Ribosomes 29 Cytoplasm • Clear, gelatinous fluid inside a cell • Majority of chemical reactions take place • Suspends cells organelles 30 Assembly and Transport • The endoplasmic reticulum: A series of highly folded membranes – Where cellular chemical reactions take place – Like a large workspace – Smooth ER • No Ribosomes attached • Involved in production and storage of lipids – Rough ER • Ribosomes attached • Involved in production of proteins Assembly and Transport • Proteins made in the Rough ER may: – form part of the plasma membrane – be released from the cell – transported to other organelles 5/3/2017 33 Assembly and Transport • The Golgi apparatus: flattened system of tubular membranes and vessicles – Modifies proteins – Sorts and packages proteins • It’s kind of like the post office: Sorts the mail and sends it to the right place 35 • Vacuoles – membrane-bound spaces used for temporary storage of materials – Store food, enzymes, waste products • Plant cells usually have one large vacuole, animal cells have many smaller cells 36 37 • Lysosomes – organelles that contain digestive enzymes – Digest organelles, food particles, and engulfed viruses or bacteria – Can fuse with vacuoles and dispense enzymes into vacuole 38 Lysosomes and recycling • Lysosomes are organelles that contain digestive enzymes – digest food particles, organelles and engulfed viruses or bacteria • Membrane prevents digestive juices from destroying the cell • Can fuse with vacuoles and digest the contents. • Can also digest cells that contain them. – i.e. tadpole’s tail 40 Energy Transformers • For all the cellular processes to happen, energy is needed • Two organelles provide that energy: – Choloroplasts (in plants) – Mitochondria (in animals and plants) Chloroplasts • cell organelles that capture light energy and produce food to store for later – Belongs to a group of plant organelles called plastids which are used for storage – Located in cells of green plants and some protists – Double membrane – Folded inner membrane forms stacks of grana/thylakoids 42 Chloroplasts • In the thylakoid membrane there is the green pigment called Chlorophyll – Traps light energy – Gives leaves and stems their green color • Plastids – Starch – Lipids – Chlorophyll 44 • Mitochondria – membrane-bound organelles in plant and animal cells – Transform energy for the cell – Highly folded inner membrane • Energy storing molecules produced in inner folds 45 Structures for Support and Locomotion • Cytoskeleton: forms the framework of the cell – Maintains shape – Assist organelles in moving from place to place – Composed of: • Microtubules: thin hollow cylinders made of protein • Microfilaments: thin, solid protein fibers 47 5/3/2017 48 Structures for Support and Locomotion • Cilia and flagella : Structures that aid in locomotion and feeding. – Composed of pairs of microtubules, with a central pair surrounded by 9 additional pairs. – Cilia are short, numerous, hairlike projections that move in a wavelike motion – Flagella are longer projections, move in a whip-like motion. Cilia 50 Flagella 51 The End 53