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Transcript
Anti-AP2M1 monoclonal antibody, clone 2D23 Cat.No: DCABH-872 Lot. No. (See product label) PRODUCT INFORMATION Product Overview Mouse monoclonal to AP2M1 Antigen Description Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y -X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Tyr-156 in membraneassociated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling. Immunogen Recombinant full length Human AP2M1 produced in HEK293T cells (NP_001020376). Isotype IgG2a Source/Host Mouse Species Reactivity Human Clone 2D23 Purity Protein A purified Purification This antibody was purified from mouse ascites fluids by affinity chromatography. Conjugate Unconjugated Applications WB, Flow Cyt Positive Control HEK293T cell lysate transfected with pCMV6-ENTRY AP2M1 cDNA; HEK293T cells transfected with an AP2M1 overexpress plasmid Format Liquid Size 100 μl Buffer pH: 7.30; Preservative: 0.02% Sodium azide; Constituents: 48% PBS, 1% BSA, 50% Glycerol Storage store at -20°C. Avoid repeated freeze / thaw cycles. Ship Shipped at 4°C. Creative Diagnostics. All rights reserved 45-1 Ramsey Road Shirley, NY 11967, USA Tel: 1-631-624-4882 Fax: 1-631-938-8221 E-mail: [email protected] www.creative-diagnostics.com 1 Antigen Gene Information Gene Name AP2M1 adaptor-related protein complex 2, mu 1 subunit [ Homo sapiens ] Official Symbol AP2M1 Synonyms AP2M1; adaptor-related protein complex 2, mu 1 subunit; CLAPM1; AP-2 complex subunit mu; AP 2 mu 2 chain; AP50; clathrin adaptor complex AP2; mu subunit; clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; clathrin associ Entrez Gene ID 1173 mRNA Refseq NM_001025205 Protein Refseq NP_001020376 MIM 601024 UniProt ID Q96CW1 Chromosome Location 3q28 Pathway Arf1 pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Function lipid binding; protein binding; transporter activity; Creative Diagnostics. All rights reserved 45-1 Ramsey Road Shirley, NY 11967, USA Tel: 1-631-624-4882 Fax: 1-631-938-8221 E-mail: [email protected] www.creative-diagnostics.com 2