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Exam 2 Objectives Bio 101 Summer, 2011 1) The Working Cell i) Membrane Structure and Function (1) Explain how membranes help organize the chemical activities of a cell. (2) Relate the structure of phospholipid molecules to the structure and properties of cell membranes. (3) Describe the fluid mosaic structure of cell membranes. (4) Describe the diverse functions of membrane proteins. b) Define diffusion and describe the process of passive transport. i) Explain how transport proteins work in facilitated diffusion. ii) Explain how osmosis can be considered to be the diffusion of water across a membrane. iii) Distinguish between hypertonic, hypotonic, and isotonic solutions. (1) Explain how plant and animal cells change when placed into hypertonic or hypotonic solutions. iv) Compare and contrast the processes of facilitated diffusion and active transport. v) Distinguish between exocytosis, endocytosis, and receptor-mediated endocytosis. 2) Introduction to the Cell a) Define cell theory and briefly describe the discoveries that led to its development. b) Explain why there are upper and lower limits to cell size. c) Distinguish between the structures of prokaryotic and eukaryotic cells. d) Explain why compartmentalization is important in eukaryotic cells. e) Compare the structures of plant and animal cells. Note the function of each cell part. f) Organelles of the Endomembrane System i) Describe the structure and functions of the: (1) nucleus, (2) endomembrane system, (smooth and rough endoplasmic reticula, Golgi apparatus, lysosomes, and vacuoles). ii) Energy-Converting Organelles (1) Compare the structures of chloroplasts and mitochondria. (2) Compare the functions of chloroplasts and mitochondria. g) The Cytoskeleton and Related Structures i) Explain the functions of the cytoskeleton. ii) Explain how the structure of cilia and flagella relate to their functions. h) Cell Surfaces and Junctions i) Compare the structures and functions of cell surfaces and intercellular junctions of plant and animal cells. i) Functional Categories of Organelles i) Describe the four functional categories of eukaryotic organelles, noting which organelles are in each group. j) Describe the three fundamental features of all life forms on our planet. 1 of 2 Exam 2 Objectives Bio 101 Summer, 2011 3) Enzymes & Reactions a) Define and compare endergonic and exergonic reactions. Explain how cells use cellular respiration and energy coupling to survive. b) Explain how ATP functions as an energy shuttle. c) How Enzymes Function i) Explain how enzymes speed up chemical reactions. ii) Describe the structure of an enzyme-substrate interaction. iii) Explain how the cellular environment affects enzyme activity. iv) Explain how competitive and noncompetitive inhibitors alter an enzyme’s activity. v) Explain how certain poisons, pesticides, and drugs inhibit enzymes. d) Describe the central role of chloroplasts and mitochondria in harvesting energy and making it available for cellular work. Key Terms active transport ATP cellular metabolism concentration gradient endocytosis energy exocytosis facilitated diffusion fluid mosaic hypertonic hypotonic isotonic osmoregulation osmosis passive transport plasmolysis receptors selective permeability tonicity cell theory cell wall cellular metabolism central vacuole chloroplasts chromatin chromosomes cilia cytoplasm cytoskeleton endomembrane system endoplasmic reticulum (ER) eukaryotic cells extracellular matrix flagella Golgi apparatus lysosome magnification / resolution mitochondria nuclear envelope nucleoid region nucleolus nucleus organelles plasma membrane prokaryotic cells receptor-mediated endocytosis prokaryotic flagella ribosomes rough ER (RER) smooth ER (SER) tight junctions transport vesicle vacuoles vesicles active site ATP coenzyme cofactors competitive inhibitor endergonic reactions energy energy coupling energy of activation (EA) enzyme exergonic reaction induced fit noncompetitive inhibitor phosphorylation substrate gap junctions / plasmodesmata 2 of 2