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NORC Adipocyte Biology and Molecular Nutrition Core Services
Request for Core Use
Requestor Name:
Campus Box:
Date of Request:
Phone:
Fax:
E-mail:
Summary of Project:
Funding Source (Agency/Number):
PI:
PLEASE ATTACH A COPY OF HUMAN AND/OR ANIMAL STUDY PROTOCOL APPROVAL LETTER(S)
Questions please contact: Terri Pietka (362-8469; [email protected]) or
Nada Abumrad (747-0348; [email protected])
Enter the approximate number of analyses needed for each service below.
a. Cells for Culture:
3T3-L1 ____ 3T3-F442A ____ OP9 ____ HIB1B _____ LS14 _____ LiSa-2 _____ SBGS _____
C2C12 ____ HSMM ____
b. Adipose Tissue Morphology:
Cell Size: ____ Cell Number: ____
c. Gene Expression Analyses:
RNA Extraction? _______
Number of Samples: _________
Type of Tissue: ________
Pathways of Interest:
Autophagy
ECM/Fibrosis
FA Metabolism
Angiogensis
Glucose Metabolism
Inflammation
ER Stress
Other (Please describe below)
RT-PCR details (e.g. genes to measure, tissues, etc.):
Total number of genes requested: ____________
d. Protein Expression Analyses:
Lysate Preparation? _______
Number of Samples: _________
Type of Tissue: ________
Pathways of Interest:
mTOR Signaling
ER Stress
Insulin Signaling
Inflammation
Other (please describe below)
Western Blotting details (e.g. proteins to measure, tissues, etc.:
Total number of proteins to measure: _______________
e. Substrate Metabolic Assays
Gucose uptake_______ Glycolysis______ Glucose Oxidation ______ Glycogenesis______
Fatty Acid Uptake______ Fatty Acid Oxidation______ Lipid Incorporation______ Lipolysis______
f. Mitochondrial Physiology:
Type of analyses: isolated mitochondria ________ Tissue _________
Tissue type _________
Substrate/inhibitors requested:
Glutamate/Malate______ Oligomycin ______ Pyruvate ______ Rotenone ______ Succinate _____
Antimycin A______ Uncoupling _______
Number of samples to be measured _______
g. Microscopy/Cell Imaging:
Briefly describe measurements needed:
h. Training:
Biochemical Characterizations (circle assay(s)):
Glucose uptake
Glycolysis
Glucose Oxidation
Glycogenesis
Fatty Acid Uptake
Fatty Acid Oxidation
Lipid Incorporation
Lipolysis
Triglycerides
Cellular trafficking and imaging
Autophagy Analysis
Specifics:
i. Consultation:
Brief project description:
Mitochondrial Function/Physiology (circle
assay(s)):
Respiration
ROS Production
Oxidative Stress
ATP Production
Mitochondrial Membrane Potential
Mitochondrial Calcium Uptake
j. Equipment Usage:
ABI-7500 Fast _____ LiCor Odyssey _____ Tissue Culture Facility _____ Nikon TE2000U Microscope
_____ Spectroscopy (Bio-Tek/NanoDrop) _____ Oxygraph 2K _____
Qiagen TissueLyser II ______
Special instructions, comments, etc. for any of the above services:
PLEASE CITE: NORC GRANT DK056341 IN ALL PUBLICATIONS RESLULTING FROM THIS
EFFORT.
Return completed form to:
Terri Pietka (362-8469; [email protected]) or
Nada Abumrad (747-0348; [email protected])
Campus Box 8031
Approval: ______________ Date: _______________ Priority: ________________