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Protein Synthesis Study Questions Chapter 8 GENERAL INFORMATION: NUCLEIC ACIDS AND PROTEINS 1. 2. 3. 4. 5. 6. What is the monomer of nucleic acids? What are they composed of? What are 2 differences between the monomers of RNA and DNA? What are proteins made of? What determines the function of a protein? What type of bond holds amino acids together in a protein? Describe the relationship between nucleic acids and proteins. STAGES OF PROTEIN SYNTHESIS 7. Describe the 3 steps involved in making RNA. 8. What is the name of the process that makes RNA? 9. Describe the 3 steps involved in RNA processing. 10. What is the purpose of RNA processing? 11. Describe the 3 steps involved in using RNA to make proteins. 12. What is the name of the process that makes proteins? 13. What happens at the A site? 14. What happens at the P site? 15. What happens at the E site? RNA 16. Name the 3 types of RNA. 17. Which RNA is a copy of one strand of DNA? 18. Which RNA makes the translation site? 19. Which RNA becomes charged in the cytoplasm? 20. Which RNA contains an anticodon? 21. Which RNA determines the amino acid sequence? 22. Which RNA makes the A, P, and E sites? 23. Which RNA carries amino acids to be assembled into a protein? 24. Which RNA is broken down after the protein is made? 25. Draw a charged tRNA with the anticodon CCA. 26. List all mRNA codons that do not have corresponding tRNA anticodons. Why is this? MUTATIONS 27. Which type of mutation does not show any effects? 28. Which type of mutation occurs when one or more bases are changed? 29. Which two mutations result in a different amino acid sequence? 30. Which mutation results in an incomplete protein? 31. Which mutation is caused by an addition or deletion of a base? 32. How do mutations affect protein synthesis? PRACTICE QUESTIONS 33. If the sequence on one strand of DNA is TAG, what is the sequence on the other strand of DNA? 34. If the mRNA sequence is AUC, what is the tRNA anticodon? 35. If the tRNA anticodon is UCG, which amino acid will it carry? 36. If the DNA sequence is GAT, what is the mRNA sequence? 37. If the DNA sequence is TAC, what is the tRNA anticodon? 38. If the amino acid is lysine, write all possible tRNA anticodons. 39. If the mRNA sequence is GAU, what is the DNA sequence is came from? 40.If the tRNA anticodon is CAU, what is the mRNA codon it will attach to? Answers to Protein Synthesis Study Questions 1. 2. 3. 4. 5. 6. nucleotides; 5 carbon sugar, phosphate, nitrogenous base RNA has ribose sugar and uracil base; DNA has deoxyribose sugar and thymine base amino acids sequence of amino acids peptide bonds DNA makes RNA, RNA makes proteins 7. a) Initiation- RNA polymerase attaches to DNA promoter region, unwinding it. b) Elongation- RNA polymerase adds complimentary RNA bases c) Termination- RNA polymerase reaches DNA terminator region, releasing RNA 8. transcription 9. a) poly-adenine tail added to help mRNA leave nucleus b) methyl-guanine cap added to help mRNA attach to ribosome c) splicing removes introns (segments that don’t code for correct amino acid) 10. modify RNA for efficient function 11. a) Initiation- ribosome attaches to mRNA at start codon b) Elongation- charged tRNAs bring amino acids to ribosome c) Termination- ribosome reaches stop codon and releases protein and RNA 12. translation 13. charged tRNA brings in amino acid 14. protein is held and released 15. uncharged tRNA exits 16. messenger, ribosomal, transfer 17. mRNA 18. rRNA 19. tRNA 20. tRNA 21. mRNA 22. rRNA 23. tRNA 24. all 25. check your drawing with me 26. UAA, UAG, UGA- they are no tRNA anticodons for stop codons 27. point silent 28. point 29. point missense 30. point nonsense 31. frameshift 32. they change the mRNA sequence, which changes the type of protein made 33. ATC 34. UAG 35. serine 36. CUA 37. UAC 38. UUU, UUC 39. CTA 40. GUA