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Basic Structure of a Cell Prokaryotic Cells •No nucleus •Archaea & Eubacteria •One circular chromosome •Extremely small Eukaryotic Cells • Has a nucleus!!! • Membrane-bound organelles • Plants, Animals, Fungi, & Protists Prokaryotes – The first Cells • Cells that lack a nucleus or membrane-bound organelles • Includes bacteria • Simplest type of cell • Single, circular chromosome Prokaryotes • Nucleoid region (center) contains the DNA • Surrounded by cell membrane & cell wall (peptidoglycan) • Contain ribosomes (no membrane) in their cytoplasm to make proteins Prokaryotes • Bacterial cells may have plasmids, small accessory rings of DNA. • Some bacteria have a capsule or a slime layer. • Most bacteria have flagella. • Some also have fimbriae that help cells attach to surfaces. • Bacteria have a great metabolic diversity. Comparison of Eukaryotic and Prokaryotic Cells Eukaryotes • Eu-, “true,” karyon, “nucleus” • Genetic material contained in a nucleus • Membrane-bound organelles • Evolved from prokaryotic cells • Includes protists, fungi, plants, and animals •Eukaryotic •No cell wall •Heterotrophic •Often highly specialized in multi-cellular organisms •May be motile DNA Nucleolus Nuclear envelope Centrosome Rough ER Cell wall Smooth ER Ribosomes CENTRAL VACUOLE Golgi complex Microfilaments Intermediate filaments Microtubules Mitochondrion Lysosome Plasma membrane CHLOROPLAST CELL WALL Wall of adjacent cell •Outer layer that maintains cell’s shape & rigidity and protects cell from mechanical damage •Made primarily of cellulose Central vacuole •Stores nutrients & breaks down waste products •Contributes significantly to plant growth Chloroplast •Contains chlorophyll, which is the primary pigment responsible for photosynthesis Organelles • Very small (Microscopic) • Perform various functions for a cell • Found in the cytoplasm • May or may not be membrane-bound Cell Wall • Nonliving layer that supports and protects the cell • Found in plants, fungi, & bacteria • Made of cellulose in plants • Made of peptidoglycan in bacteria • Made of chitin in Fungi Cell wall Cell Membrane in Plants Cell membrane • Lies immediately against the cell wall in plant cells • Pushes out against the cell wall to maintain cell shape Cytoplasm of a Cell cytoplasm • Jelly-like substance enclosed by cell membrane • Provides a medium for chemical reactions to take place More on Cytoplasm cytoplasm • Contains organelles to carry out specific jobs • Found in ALL cells The Control Organelle - Nucleus • Controls the normal activities of the cell • Contains the DNA in chromosomes • Bounded by a nuclear envelope (membrane) with pores • Usually the largest organelle More on the Nucleus Nucleus Chromosomes: • Tightly coiled DNA & Protein • Contain hereditary material (genes) • Chromosomes not dividing called chromatin • Each type of organism has a certain # of them • Humans = 46 (23 pairs) • Fruit fly = 8 (4 pairs) Inside the Nucleus The genetic material (DNA) is found DNA is spread out And appears as CHROMATIN in non-dividing cells DNA is condensed & wrapped around proteins forming as CHROMOSOMES in dividing cells What Does DNA do? DNA is the hereditary material of the cell Genes that make up the DNA molecule code for different proteins Nuclear Envelope • Double membrane surrounding nucleus • Also called nuclear membrane • Contains nuclear pores for materials to enter & leave nucleus • Connected to the rough ER Nuclear pores Nucleolus • Inside nucleus • Cell may have 1 to 3 nucleoli • Disappears when cell divides • Makes ribosomes that make proteins Endomembrane System • System of transport throughout the cell • Nuclear envelope • Endoplasmic reticulum • Golgi apparatus • Lysosomes • Vacuoles Endomembrane System Includes nuclear membrane connected to ER connected to cell membrane (transport) Endoplasmic Reticulum - ER • Network of hollow membrane tubules throughout the cytoplasm • Connects to nuclear envelope & cell membrane • Functions: – Transports materials through the cell – Contains enzymes that allow chemical reactions – Wastes & other toxic chemicals are made harmless • Two types: – Smooth ER – Rough ER The endoplasmic reticulum Rough ER: •Studded with ribosomes •Makes membrane proteins and proteins for export out of the cell Smooth ER: •Lacks ribosomes on its surface •Is attached to the ends of rough ER •Makes cell products that are used inside the cell •Site of synthesis of lipids (including phospholipids and steroids) •Destroys toxic substances Ribosomes • • • • • Made of PROTEINS and rRNA Made in the nucleolus Found mostly on ER but also floating in cytoplasm Found in all types of cells “Protein factories” for cell • Join amino acids to make proteins (process called protein synthesis) Golgi Complex (or Apparatus) • Flattened membrane sacs • Present in almost all cells • Receives protein and lipid-filled vesicles from ER • Modifies, sorts, and packages molecules from ER for storage or transport out of the cell Vacuoles and Lysosomes Vacuoles (large) and vesicles (small) are membranous sacs in the cell that store substances. Lysosomes are vesicles produced by the Golgi apparatus. Lysosomes contain hydrolytic enzymes and are involved in intracellular digestion. Vacuoles • Fluid filled sacks for storage • Small or absent in animal cells • Plant cells have a large Central Vacuole • In plants, they store Cell Sap • Includes storage of sugars, proteins, minerals, lipids, wastes, salts, water, and enzymes • No vacuoles in bacterial cells • Many protists have contractile vacuoles Contractile Vacuole • Found in unicellular protists like paramecia • Regulate water intake by pumping out excess (homeostasis) • Keeps the cell from lysing (bursting) Lysosomes • Contain digestive enzymes • Break down food, bacteria, and worn out cell parts for cells • Some cells (i.e. certain types of protists) take in food by phagocytosis • Lysosomes digest the food and get rid of wastes Energy-Related Organelles The two energyrelated organelles of eukaryotes are mitochondria and chloroplasts . Both organelles house energy in the form of ATP. Mitochondrion (plural = mitochondria) • Found in all eukaryotic cells • “Powerhouse” of the cell • Site of Cellular Respiration: generate cellular energy (ATP) by burning glucose • More active cells like muscle cells have MORE mitochondria Mitochondrion (plural = mitochondria) Structure: • Surrounded by a DOUBLE membrane • Folded inner membrane called cristae (increases surface area for more chemical reactions) • Interior called matrix • Has its own DNA Interesting Fact --• Mitochondria Come from cytoplasm in the EGG cell during fertilization Therefore … • You inherit your mitochondria from your mother! Chloroplasts • Organelle found only in producers • Never in animal, fungal, or bacterial cells • Contains enzymes & pigments for Photosynthesis • Photosynthesis: Use energy from sunlight to make food (glucose) • Energy from sun stored in the Chemical Bonds of Sugars • Contains its own DNA Chloroplasts Structure: • Surrounded by DOUBLE membrane • Outer membrane smooth • Inner membrane modified into sacs called Thylakoids • Thylakoids contain Chlorophyll – a green pigment which absorbs energy from the sun • Thylakoids are organized into stacks called Grana and are interconnected • Stroma – gel like material surrounding thylakoids Process that uses solar energy to convert carbon dioxide and water into glucose and oxygen Structure of Chloroplast: • Surrounded by double membrane • Outer membrane is smooth • Inner membrane is modified into sacs called Thylakoid Discs • Thylakoid membranes contain Chlorophyll • Thylakoids are organized into stacks called Grana and are interconnected • Stroma – gel like material surrounding thylakoids Outer membrane Granum Thylakoid discs Inner membrane Stroma Cytoskeleton • Network of filaments and tubules that extends from the nucleus to the plasma membrane • Made of proteins • Helps cell maintain cell shape • Also help move organelles around • Types: – Microfilaments – threadlike and made of Actin MICROTUBULES – Microtubules – tubelike and made of Tubulin MICROFILAMENTS – Intermediate filaments Centrioles • Found only in animal cells • Paired structures near nucleus • Made of bundle of microtubules • Appear during cell division forming mitotic spindle • Help to pull chromosome pairs apart to opposite ends of the cell Cilia & Flagella • Made of protein tubes called microtubules • Microtubules arranged (9 + 2 arrangement) • Function in moving cells, in moving fluids, or in small particles across the cell surface Cilia & Flagella • Cilia are shorter and more numerous on cells • Flagella are longer and fewer (usually 1-3) on cells Cell Movement with Cilia & Flagella