Download Protein Synthesis Practice

Replication Transcription and Translation!!!!
Name:_______________________________________Per:__________Date:__________
Now that you’re experts on the process of DNA replication and protein synthesis, let’s put it to the test! You’re
ready to become a professional DNA/RNA code breaker. Write the complimentary base pairs for the
segments of DNA or RNA below.
DNA Replication
REMEMBER: DNA copies itself using DNA polymerase to add new complimentary nucleic acid bases.
1. A T C G G G C G A C C G T A C
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2. T G C C G T A G C T A G C T A G
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Transcription
At this stage mRNA copies information from a strand of DNA. RNA Polymerase adds matching RNA bases.
3. A C G A T C G A T A G C
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4. C G A T C G T A G A T C G A T A G
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Translation
Now mRNA takes the transcribed section out of the nucleus and is translated in the ribosome by tRNA. Write
the complimentary tRNA anticodons for each mRNA codon.
5. AUG
GUA
GAU
CUA
CGA
CGA
UGA
6. AUG
CGA
CCU
CUC
CGU
AGA
CGU
UAG
Now, write the names of the amino acids that will correspond to each mRNA codon for these genes.
Protein A:
AUG
GUA
CGA
UGA
methionine_________________________________________________
Protein B:
AUG
CGA
CCU
AGA
CGU
UAG
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GOOD WORK! Now let’s really try to trick you.
Given the following mRNA strands, draw a circle around the START CODONS and the STOP CODONS.
Number the 3-base pair codons in between. A whole protein need to have a series of codons between a start
(AUG) and a stop codon (UGA, UAG or UAA). Which strands will build whole proteins?
Example: This strand builds a whole protein
7. CGG AUG
CGU CGC AAC GAG AGC GCG CGC GGA
1
2
3
4
5
6
8
9
UGA
CCGGAAC
Put a square around the start codon, then circle each additional codon, number it and label its amino acid.
Square the stop codon when you get there. If there is no start or no stop, then it is not a complete protein.
8.
AUGGAGGAGAUCUCUCGCGCUAGAGAUCGCGCGUAG C
9. C C C G G G G A A A U C G C U A G C C G G C U U A G G A U C
10. A U G G G G A U A G G A U G A G A U A G C G A U A G A G A G A U C
11. C G C G U U A U G C C G G G C C C C G G G U G A G C U G
12. A G A G U C U C U C G A A U G C G C A A A U A G A G U C G U A U G G
Need Help? You Tube Crash Biology: Number 10: http://www.youtube.com/watch?v=8kK2zwjRV0M
Number 11: http://www.youtube.com/watch?v=itsb2SqR-R0
Lastly, decode these DNA strands to mRNA then amino acids to make proteins using the
amino acid decoder chart. The same chart is available in your books on p. 367.
1.
DNA: TAC GCT CCG GGC AGA GGG ATT
mRna:
Amino Acid:
2. DNA:
mRna:
Amino Acid:
TAC AAA GTA GTC ACT
3. DNA:
mRna:
Amino Acid:
TAC AAA GTG AAG GTT ACT