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Transcript
Chapter 8 Dynamic Cells• MHR CHAPTER AT A GLANCE Student Book Page 315 Answers (a) The cell membrane is composed of a phospholipid bilayer in which proteins (and molecules such as cholesterol) are embedded. Carbohydrate chains are attached to the outer surface. The bilayer itself has a fluid structure. (b) A semi-permeable membrane allows the passage of some types of materials, but not others, often because the materials are too large, or because they are too strongly hydrophilic or charged. (c) If the cell membrane was permeable to all materials, harmful substances could enter the cell and the cell would lose required materials, such as food molecules. If the cell membrane was impermeable to all materials, the cell would be a closed system and therefore would not be functional. (d) Some membrane proteins function in facilitated diffusion. These proteins, called transport proteins, include channel proteins, which allow the passage of ions down a concentration gradient and across the membrane; and carrier proteins, which change conformation upon binding a molecule, thereby allowing the molecule to pass through the membrane, again, moving with a concentration gradient. Active transport can move materials against a concentration gradient by use of carrier proteins; active transport requires cellular energy. Receptors on the cell surface are involved in receptor-mediated endocytosis. (e) Particles that are in equilibrium are distributed randomly in a space. While the particles are in constant motion, there is no net flow of particles from one area to another (such as from the external environment to the cytoplasm). (f) Carrier proteins fuelled by cellular energy (active transport) can pump materials against a concentration gradient. (g) The net movement of water molecules will always be from a region of lower solute concentration to a region of higher solute concentration. (h) Endocytosis includes phagocytosis (cell eating) and pinocytosis (cell drinking). With phagocytosis the cell membrane surrounds and engulfs particulate matter. The cell membrane then closes in on itself, and the particle is enclosed in a vesicle, which pinches off inside cell. Some single-celled organisms, such as amoeba, feed using phagocytosis. White blood cells defend the body by phagocytising bacteria. With pinocytosis, fluid droplets are engulfed by the cell membrane. A pocket pinches off as a vesicle in the cytoplasm. The vesicle shrinks as the fluid particles diffuse into the cytoplasm. TR 8-61 With endocytosis, vesicles containing materials fuse with the cell membrane. The vesicle’s contents are then released outside the cell. Exocytosis is used to rid the cell of wastes or secrete cell products. (i) Some shapes (flat and/or long, for instance) have a high surface-area-to-volume ratio compared to others (completely spherical, for instance). (j) Diffusion rates within a hypothetical really large cell would be quite slow. It would therefore be too inefficient for cells to grow extremely large (such cells would have low surface-area-to volume ratios). Multicellular organisms can only grow large because they are composed of many cells. Prepare Your Own Summary • Answers will vary. A sample map is provided here. multicellularity limits cell size surface area-to-volume ratio determine surface area membrane structure membranes transport in/out of cell semi-permeable phospholipid bilayer endocytosis/ exocytosis fluid nature proteins receptors osmosis channel proteins carrier proteins diffusion transport down concentration gradient transport against concentration gradient does not use cellular energy requires cellular energy passive transport active transport facilitated diffusion • Answers will vary, but should address the semi-permeable nature of cell membranes and the ability of active transport to move materials across the membrane against concentration gradients. TR 8-62 MHR • Unit 3 Cycling of Matter in Living Systems Type of transport How transport-type works Factors influencing direction of transport osmosis water molecules pass through cell membrane concentration of solutes in or around the cell diffusion diffusion of molecules through the cell membrane direction of transport is down a concentration gradient facilitated diffusion transport proteins enable ions or molecules to pass through the cell membrane by way of channel proteins or carrier proteins, without using cellular energy direction of transport is down a concentration gradient active transport transport of molecules or ions through the cell membrane by use of carrier proteins; process requires cellular energy direction of transport may be against concentration gradient (or with it) endocytosis/ exocytosis pinocytosis and phagocytosis independent of include ways in which cells concentration gradients engulf and take in large particles such as fluid droplets (pinocytosis) or dead cells or bacteria (phagocytosis); exocytosis allows transport of proteins and other macro-molecules and wastes out of the cell • Answers will vary. Consider having students present some of their poems or songs to the class. CHAPTER 8 REVIEW ANSWERS Student Book Pages 316–317 Key Terms See glossary in student book. Understanding Key Concepts 1. The cell membrane consists of two layers of phospholipid molecules. 2. a, c, and d are open systems because they allow the inward and outward flow of matter. 3. (a) This figure illustrates facilitated diffusion using a carrier protein. (b) carrier protein cholesterol membrane phospholipid 4. (a) A bacterial cell would be transported by endocytosis. It could be stored in a vacuole or transported in a vesicle. (b) Carbon dioxide is transported by diffusion. (c) Water is transported by osmosis. (d) Sodium ions are transported by active transport or facilitated diffusion. 5. The particle model of matter states that all matter is made up of particles that are in constant, random motion. This model describes the water and dissolved molecules that fill and surround cells. It is the molecules’ random movement that causes those with sufficient kinetic energy to spread out from a concentrated area and distribute themselves evenly. Diffusion is the term used to describe the molecules’ net movement down the gradient. 6. Channel proteins - transport ions down a concentration gradient; carrier proteins enable active transport or facilitated diffusion; receptors (proteins with marker molecules) - cell-cell recognition, communication, protection from infection, receptormediated endocytosis. 7. Phospholipid molecules have a head end that readily dissolves in water and an oily tail end that does not dissolve in water. In water, each phospholipid molecule automatically orients itself so that its head end points toward the water molecules and the tail end points toward other phospholipid tails. A bilayer arrangement, with the tails sandwiched in the middle of the layer and the head ends pointing outward, is the most stable arrangement of the phospholipids. 8. A dialysis membrane contains very small pores that are large enough to allow the diffusion of dissolved ions and other small molecules, but too small to allow the movement of red or white blood cells. As the patient’s blood passes through tubing made from a dialysis membrane, small dissolved wastes can diffuse out and needed materials diffuse in, while blood cells are retained. 9. Surface area-to-volume ratio limits cell size. As cell size increases, this ratio gets lower, and as a result, nutrients and other important materials cannot diffuse fast enough to areas in the cell where they are required. As well, as cell size increases it is difficult for wastes to be expelled fast enough. 10. Both receptor-mediated endocytosis and active transport move substances into the cell that cannot be transported across the membrane by passive diffusion. Both processes use membrane proteins to recognize specific substances for transport, and both consume cellular energy in the process. However, active transport is used to move very small dissolved atoms or molecules via carrier proteins embedded in the cell membrane. In contrast, receptor-mediated endocytosis is used to transport bulky items, such as small cells or droplets of fluid. Rather than moving Chapter 8 Dynamic Cells• MHR these items through a pore, they are taken in through the formation of a vesicle or vacuole. Developing Skills 11. (a) Hypertonic Hypotonic Isotonic potato is soft potato is firm potato is firm (b) The potato cube in the relatively hypertonic solution will become soft, while the other potato cubes will be firm. 12. Models should include phospholipids, different types of proteins, and carbohydrate chains. 13. (a) Manipulated variables – type of alcohol and concentration Responding variable – leakage of pigment from beet root cells as measured by absorbance Amount of Beet Pigment Extracted by Alcohols as Measured by Absorbance Absorbancy (nm) 700 600 500 Methanol Ethanol Propanol 400 300 200 100 0 02 04 06 08 Concentration (%) 0 100 (b) Methanol was most damaging to beet cell membranes. (c) Methanol seems to damage the membranes the most, as determined by the amount of pigment leaking from the beet root cells. In all but one trial, methanol was equal to or more damaging than ethanol. Methanol was equal to or more damaging than propanol in all trials. Problem Solving/Applying 14. Some possible household items that contain or have features of a semi-permeable membrane include: – cheesecloth, which allows fluid to pass through the fabric mesh, while retaining large cheese curds during cheese making – kitchen strainers or colanders, which allow fluid to pass through wire mesh or holes in a metal bowl during draining of foods cooked or rinsed in water – vacuum cleaners that have air vents lined with foam padding that allow air to pass through while TR 8-63 retaining dust particles – dust masks, which prevent inhalation of dust particles and other airborne substances and that are made of paper, which is permeable to air but not to small particles 15. Use one solution to fill the dialysis tubing, then tie the ends and immerse it in the other solution. Take and record the mass of the “cell” over time and record the cell’s change in mass. Changes in the amount of fluid filling the cell will indicate in which direction osmosis has occurred. If the cell increases in mass, this indicates that water has flowed into the cell by osmosis. Therefore, the solution inside the cell must have the stronger solute concentration. Conversely, if the cell decreases in volume, this indicates that water has flowed out of the cell by osmosis. Therefore, the solution outside the cell must have the stronger solute concentration. 16. Answers will vary. Students should be able to clearly distinguish between their hypotheses and predictions. One experiment could be to place unshelled eggs in solutions of different solute concentrations. 17. The organisms living in the dried up ponds will likely lose water to the environment if they do not have adaptations to counteract this pressure. The organisms could dehydrate and die. Critical Thinking 18. The cell membrane acts as a physical barrier that excludes many materials from entering or exiting the cell. Substances that are large or electrically charged cannot easily diffuse through the membrane. Despite this barrier, the cell is still an open system. Very small dissolved molecules can diffuse passively through the membrane. Some other items are recognized by membrane proteins and are transported into or out of the cell by facilitated diffusion, active transport, or endocytosis/exocytosis. 19. Such a cell would have no protection from substances in the environment. Also, this cell would not be able to “hold on” to important nutrients and other molecules. The cell would not be able to form proper compartments in which biological reactions could take place. 20. The flat cell shape provides a thin surface through which oxygen must diffuse to reach the blood. The thinner the shape, the more easily the oxygen diffuses. Therefore, this cell shape improves the ability of our lungs to provide oxygen to the bloodstream. 21. Experiments in which human and mouse cells were fused illustrated the fluidity of cell membranes. This experiment, as described in the Think&Link Investigation 8–A: Fusing Fluid Cell Membranes, demonstrated that membrane proteins move laterally through the membrane. Micrographs of cells phagocytosing particles also suggest that the cell membrane has a fluid structure, since the membrane stretches and folds in this process.