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Transcript
Inexpensive Cell Migration Inquiry Lab using Danio rerio – Kate M. Cooper Zebrafish Cell Migration • How do cells move within the body? White blood cells have to migrate to hunt down invading bacteria • Largest fraction of your white blood cells are neutrophils • “Migration” means a cell will move from one place to another under its own power • Neutrophil chasing S. aureas movie Neutrophils will also migrate in response to a wound • Zebrafish embryo neutrophil migration movie M X Wounding the zebrafish X Neutrophils can be visualized with Sudan Black lipophilic dye Figure from a student lab report. This system can be used to investigate molecules important for directional cell migration Discussion Question: What are some of the structures in the cell that might be necessary for directional cell migration? Brainstorm a list with your group. Hint: think about how the cell would need to recognize a signal to move and how it might physically move. Cytoskeleton • Discussion question: What are the three main components of the cell cytoskeleton? What are similarities and differences between their structures? Torsen Wittman, www.cellimagelibrary.org Goal in lab next week: • Design and test a hypothesis about the cytoskeleton and neutrophil migration in Danio rerio. • Inhibitors to cytoskeletal components and 3-day-old zebrafish embryos will be provided. • Remember you will be doing a full write up (title, abstract, introduction, methods, results, discussion) for this lab, therefore, make sure you have at least two or three journal articles you’ve found ahead of time that you might use in your introduction or discussion section.