* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download protein synthesis slides - week 1
Real-time polymerase chain reaction wikipedia , lookup
Western blot wikipedia , lookup
Molecular cloning wikipedia , lookup
Polyadenylation wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Eukaryotic transcription wikipedia , lookup
Peptide synthesis wikipedia , lookup
RNA polymerase II holoenzyme wikipedia , lookup
Protein–protein interaction wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Metalloprotein wikipedia , lookup
DNA supercoil wikipedia , lookup
Non-coding DNA wikipedia , lookup
Protein structure prediction wikipedia , lookup
Amino acid synthesis wikipedia , lookup
Transcriptional regulation wikipedia , lookup
Silencer (genetics) wikipedia , lookup
Two-hybrid screening wikipedia , lookup
Proteolysis wikipedia , lookup
Biochemistry wikipedia , lookup
Point mutation wikipedia , lookup
Messenger RNA wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Gene expression wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Genetic code wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Wednesday – 11.9.16 Warm-up: Answer numbers 1-2 using the protocol outlined below. Warm-up #1 1. If one strand of DNA molecule has the base sequence A-G-C-T-A, the complementary strand of DNA would have the base sequence – a. b. c. d. A-G-C-T-A U-C-G-A-T U-C-G-A-U T-C-G-A-T Warm-up #2 2. A strand of DNA consists of thousands of smaller repeating units known as – a. b. c. d. lipids amino acids nucleotides polysaccharides Why should we care about Protein Synthesis? • As you watch the video, write a list of all of the uses of proteins that you see in the video. • At the end, we will see who has the longest list! • P.S. You will need this list for a homework assignment so I suggest you don’t lose it! Introduction to Protein Synthesis Amoeba Sisters Video As you watch the video clip, fill out the Venn Diagram that you were given. Why RNA is just as cool as DNA Phrase Bank A.Double-helix shape B.Adenine C.Single-stranded D.Nucleic Acid E.Deoxyribose F.Cytosine G.Ribose H.Contain a sugar I.Thymine J.Guanine K.Uracil L.Phosphate M.Double-stranded DNA RNA BOTH Protein Synthesis Booklet – pg. 1 1. Look at the cover page of your booklet. 2. Attempt to identify things you recognize from previous units. 3. Identify aspects of the diagram that are completely unfamiliar to you. Protein Synthesis Booklet – pg. 2 Directions: 1. Flip to page 2 of your booklet. 2. Fill in the blanks to the overview section using the word bank to the right. Word Bank: • • • • • • proteins DNA inherit genes DNA The sequence of nitrogen bases Overview ____________________________________ code for your ______. Certain segments of ______ code for your ________. You _____________ these from your parents. They code for the ____________ that make up your traits. Protein Synthesis Booklet – pg. 2 Overview The sequence of nitrogen bases code for your DNA . Certain segments of DNA code for your genes . You inherit these from your parents. They code for the proteins that make up your traits. Protein Synthesis Booklet – pg. 3 • Turn to page 3 of your booklet. • Fill in the missing blanks using the words below. – Nucleus, Translation, Transcription, Ribosome 1st: ________________ occurs in the ___________________ 2nd: ________________ occurs at the ____________________ Protein Synthesis Booklet – pg. 3 1st: Transcription occurs in the Nucleus 2nd: Translation occurs at the Ribosome Protein Synthesis Booklet – pg. 4 Transcription Occurs in the: Nucleus End Result: mRNA Label the diagram with the terms below: • Nucleus • Nuclear pores • DNA bases • mRNA bases DNA mRNA Protein Synthesis Booklet – pg. 2 DNA mRNA Transcription occurs in the nucleus . Amoeba Sisters Video Protein Synthesis and the Lean, Mean Ribosome Machines 2 Steps of Protein Synthesis Transcription: occurs in the ______________ • ______ is used a a template to make _______. • _______ leaves the ___________ through nuclear pores and travels to the ____________. Translation: occurs in the _____________ at the _____________. • A _______ molecule attaches to each codon in the mRNA molecule. • 1 ___________ _________is attached to each tRNA molecule. • Peptide bonds form between each amino acid to form a ___________________ __________, which is a protein. • Proteins code for your genetic ____________. 2 Steps of Protein Synthesis Transcription: occurs in the nucleus • DNA is used a a template to make mRNA. • mRNA leaves the nucleus through nuclear pores and travels to the ribosome. Translation: occurs in the cytoplasm at the ribosome • A tRNA molecule attaches to each codon in the mRNA molecule. • 1 amino acid is attached to each tRNA molecule. • Peptide bonds form between each amino acid to form a polypeptide chain, which is a protein. • Proteins code for your genetic traits. Protein Synthesis Booklet – pg. 5 Translation • • • • • • • • Label the diagram with the terms below: Nucleus Nuclear pores mRNA bases tRNA molecule tRNA bases Amino acids Peptide bond Polypeptide chain = Protein Occurs at the: Ribosome End Result: Protein mRNA tRNA + amino acids polypeptide chain proteins Protein Synthesis Booklet – pg. 2 mRNA tRNA + amino acids polypeptide chain proteins traits __Translation__ ____occurs____ at the ribosome in the cytoplasm. Friday – 11.11.16 • Take out your Protein Synthesis Warm-up Problem Handout you received Monday. • Answer 3-4 using your sticky note and the Protocol for MC Questions. Warm-up #3 3. Based on structure, in what way do DNA molecules differ from RNA molecules? a. RNA contains the base uracil; DNA does not contain uracil b. DNA is composed of two chains of nucleotides; RNA is composed of three chains of nucleotides. c. RNA is helical; DNA is branched. d. DNA is composed of four different bases; RNA is composed of three different bases. Warm-up #4 4. A DNA molecule with the base sequence A-G-CT-C-A was used as a template for the synthesis of a messenger RNA molecule. Which bases sequence correctly represents the corresponding portion of the RNA molecule? a. b. c. d. T-C-A-G-C-A U-C-G-A-G-U A-G-C-U-C-A A-T-G-A-C-T Codon Charts Codon Charts Protein Synthesis Booklet – pg. 6 1. TRANSCRIPTION __________ TRANSLATION __________ 2. TRANSCRIPTION __________ TRANSLATION __________ DNA: CCC mRNA: GGG CCC tRNA: ____ GLY amino acid: ____ DNA: mRNA: tRNA: amino acid: AAT GTA UUA CAU AAU GUA ____ ____ LEU ____ HIS ____ TAG CTG ATA AUC GAC UAU ____ ____ ____ UAG AUA ____ CUG ____ ____ ASP TYR ISO ____ ____ ____ Protein Synthesis Booklet – pg. 7 3. TRANSCRIPTION __________ TRANSLATION __________ CAT DNA: ____ mRNA: GUA CAU tRNA: ____ VAL amino acid: ____ GCA ____ CGU GCA ____ ARG ____ CTG ____ GAC CUG ____ ASP ____ Protein Synthesis Booklet – pg. 7 4. TRANSCRIPTION __________ TRANSLATION __________ DNA: mRNA: tRNA: amino acid: GCA TAG CAA CGU GUU AUC ____ ____ ____ GCA CAA UAG ____ ____ ____ VAL ARG ____ ISO ____ ____ Snowball 1. Create and write down ONE of the following sequences on your slip of paper. – DNA: A, T, C, G – mRNA: A, U, C, G – tRNA: A, U, C, G 0:01 0:02 0:03 0:04 0:05 0:06 0:07 0:08 0:09 0:10 0:11 0:12 0:13 0:14 0:15 0:16 0:17 0:18 0:19 0:20 0:21 0:22 0:23 0:24 0:25 0:26 0:27 0:28 0:29 0:30 0:31 0:32 0:33 0:34 0:35 0:36 0:37 0:38 0:39 0:40 0:41 0:42 0:43 0:44 0:45 0:46 0:47 0:48 0:49 0:50 0:51 0:52 0:53 0:54 0:55 0:56 0:57 0:58 0:59 1:00 End 2. Crumple your piece of paper into a paper ball. Snowball 3. GENTLY, throw your ball to someone across the room. 4. Open the paper ball, and complete the remaining three lines. 5. Crumple the paper back into a paper ball. 0:01 0:02 0:03 0:04 0:05 0:06 0:07 0:08 0:09 0:10 0:11 0:12 0:13 0:14 0:15 0:16 0:17 0:18 0:19 0:20 0:21 0:22 0:23 0:24 0:25 0:26 0:27 0:28 0:29 0:30 0:31 0:32 0:33 0:34 0:35 0:36 0:37 0:38 0:39 0:40 0:41 0:42 0:43 0:44 0:45 0:46 0:47 0:48 0:49 0:50 0:51 0:52 0:53 0:54 0:55 0:56 0:57 0:58 0:59 1:00 1:01 1:02 1:03 1:04 1:05 1:06 1:07 1:08 1:09 1:10 1:11 1:12 1:13 1:14 1:15 1:16 1:17 1:18 1:19 1:20 1:21 1:22 1:23 1:24 1:25 1:26 1:27 1:28 1:29 1:30 1:31 1:32 1:33 1:34 1:35 1:36 1:37 1:38 1:39 1:40 1:41 1:42 1:43 1:44 1:45 1:46 1:47 1:48 1:49 1:50 1:51 1:52 1:53 1:54 1:55 1:56 1:57 1:58 1:59 2:00 End Codon Bingo • I will read an mRNA codon. • You will translate the codon to determine which amino acid it codes for using your codon chart. • If you have that amino acid on your card, write the mRNA codon I said in that space using a dry erase marker. • Once, you have bingo, say BINGO!