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Transcript
Unit 4: DNA and Protein Synthesis
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Big Idea 3: Living systems store, retrieve, transmit, and respond to information essential to life processes
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3A: Heritable information provides for continuity of life
o 3.A.1: DNA and in some cases, RNA, is the primary source of heritable information
▪ Genetic information is transmitted from one generation to the next through DNA or RNA
▪ DNA and RNA molecules have structural similarities and differences that define function
▪ Genetic information flows from a sequence of nucleotides in a gene to a sequence of amino
acids in a protein
3C: The processing of genetic information is imperfect and is a source of genetic variation
o 3.C.1: Changes in genotype can result in changes in phenotype
▪ Alterations in a DNA sequence can lead to changes in the type or amount of the protein
produced and the consequent phenotype
▪ Errors in DNA replication or DNA repair mechanisms, and external factors, including
radiation and reactive chemicals, can cause random changes. E.g., mutations in the DNA
o 3.C.2: Biological systems have multiple processes that increase genetic variation
▪ The imperfect nature of DNA replication and repair increases variation
▪ The horizontal acquisitions of genetic information primarily in prokaryotes via
transformation (uptake of naked DNA), transduction (viral transmission of genetic
information), conjugation (cell to cell transfer), and transposition (movement of DNA
segments within and between DNA molecules) increase variation
▪ Sexual reproduction in eukaryotes involving gamete formation, including crossing-over
during meiosis and the random assortment of chromosomes during meiosis, and fertilization
serve to increase variation. Reproduction processes that increase genetic variation are
evolutionarily conserved and are shared by various organisms
My Look-Fors
3.A.1.A-Genetic information is transmitted from one generation to the next
through DNA or RNA
Genetic information is stored in and passed to subsequent generations through
DNA molecules and, in some cases, RNA molecules
Non-eukaryotic organisms have circular chromosomes, while eukaryotic
organisms have multiple linear chromosomes, although in biology there are
exceptions to this rule
Prokaryotes, viruses, and eukaryotes can contain plasmids, which are small
extra chromosomal, double-stranded circular DNA molecule
The contributions of Watson and Crick to the understanding of DNA
The contributions of Wilkins and Franklin to the understanding of DNA
The contributions of Avery-MacLeod-McCarty to the understanding of DNA
The contributions of Hershey-Chase to the understanding of DNA
DNA replication is a semi-conservative process; that is, one strand serves as the
template for a new, complementary strand
Replication requires DNA polymerase plus many other essential cellular
enzymes, occurs biodirectionally, and differs in the production of leading and
lagging strands.
DNA polymerase, ligase, RNA polymerase, helicase, topoisomerase
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3.A.1.B-DNA and RNA molecules have structural similarities and differences that
define function
DNA and RNA have 3 components: Sugar, phosphate, and a nitrogenous base
which form nucleotide units that are connected by covalent bonds to forma
linear molecule with 3’ and 5’ ends, with the nitrogenous bases perpendicular
to the sugar-phosphate backbone
DNA contains deoxyribose sugar
RNA contains ribose sugar
RNA contains Uracil in lieu of the thymine found in DNA
DNA is usually double stranded
RNA is usually single stranded
The two DNA strands in double-stranded DNA are anti-parallel in directionalilty
DNA and RNA exhibit specific base-pairing that is conserved through evolution:
Adenine pairs with thymine or uracil (A-T or A-U) and cytosine pairs with
Guanine (C-G)
Purines (G and A) have a double ring structure
Pyrimidines (C, T, and U) have a single ring structure
mRNA carries information from the DNA to the ribosome
tRNA molecules bind specific amino acids and allow information in the mRNA
to be translated to a linear peptide sequence
rRNA molecules are functional building blocks of ribosomes.
The role of RNAi includes regulation of gene expression at the level of mRNA
transcription.
3.A.1.C
RNA polymerase reads the DNA molecule in the 3’ to 5’ direction and
synthesizes complementary mRNA that determine the order of amino acids in
the polypeptide.
In eukaryotic cells the mRNA transcript undergoes a series of enzyme regulated
modifications, to include addition of a poly-A tail, a GTP cap, and the excision of
introns
Translation of mRNA occurs in the cytoplasm on the ribosome.
In prokaryotic organisms, transcription is coupled to translation of the
message. Translation involves energy and many steps, including initiation,
elongation, and termination.
Salient features of transcription and translation include: the mRNA interacts
with the rRNA of the ribosome to initiate translation at the start codon
The sequence of nucleotides on the mRNA is read in triplets called codon
Each codon encodes a specific amino acid that can be deduced by using a
genetic code chart. Many amino acids have more than one codon.
tRNA brings the correct amino acid to the correct place on the mRNA
3.C.1.A-Alterations in a DNA sequence can lead to changes in the type or
amount of the protein produced and the consequent phenotype
DNA mutations can be positive, negative, or neutral based on the effect or the
lack of effect they have on the resulting nucleic acid or protein and the
phenotypes that are conferred by the protein
3.C.1.B-Errors in DNA replication or DNA repair mechanisms and external
factors, including radiation and reactive chemicals, can cause random changes
Whether or not a mutation is detrimental, beneficial, or neutral depends on the
environmental context. Mutations are the primary source of genetic variation.
3.C.2.A-The imperfect nature of DNA replication and repair increases variation.
3.C.2.B-The horizontal acquisitions of genetic information primarily in
prokaryotes via transformation (uptake of naked DNA), transduction (viral
transmission of genetic information), conjugation (cell-to-cell transfer) and
transposition (movement of DNA segments within and between DNA
molecules) increase variation.