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8.4 Transcription KEY CONCEPT Transcription converts a gene into a single-stranded RNA molecule. 8.4 Transcription RNA carries DNA’s instructions. • The central dogma states that information flows in one direction from DNA to RNA to proteins. • replication The central dogma includes three processes. – Replication – Transcription – Translation • RNA is a link between DNA and proteins. transcription translation 8.4 Transcription • RNA differs from DNA in three major ways. – RNA has a ribose sugar. DNA:deoxyribose sugar – RNA has uracil instead of thymine. (A, C, U, G) DNA A, C, T, G – RNA is a single-stranded structure. DNA: double stranded 8.4 Transcription Transcription makes three types of RNA. • Transcription copies DNA to make a strand of RNA. 8.4 Transcription • Transcription is catalyzed by RNA polymerase. – The transcription complex recognizes the start of a gene and unwinds a segment of it. (1) – RNA polymerase and other proteins form a transcription complex. (2) start site transcription complex nucleotides 8.4 Transcription – Nucleotides (5) pair with one strand of the DNA (4). – RNA polymerase (7) reads one side of the DNA template and strings together a complementary strand of RNA nucleotides. (6) – The DNA helix winds again as the gene is transcribed. DNA RNA polymerase moves along the DNA 8.4 Transcription – The RNA strand detaches from the DNA once the gene is transcribed. (3) RNA 8.4 Transcription • Transcription makes three types of RNA. – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. 8.4 Transcription The transcription process is similar to replication. • Transcription and replication both involve complex enzymes and complementary base pairing. • The two processes have different end results. – Replication copies all the DNA; transcription copies one gene growing RNA strands a gene. – Replication makes one copy; DNA transcription can make many copies.