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Cells and Cell Structure What are Cells? Def: the smallest level at which life can exist. Discovery of the cell. Robert Hooke (1635 –1703) Hooke discovered cells by looking at cork samples through his new invention, the Microscope. He named cells after the tiny rooms that were lived in by the monks nearby monastery. Anton Von Leeuwenhoek (1632-1723) A Dutch scientist that used a microscope like Hooke’s to see that there were small living things in the water that was making people sick in his community. He called them “Animicules” Two German Scientists concluded that all plants and all animals were made of cells. Schleiden and Schwann The Cell Theory •All Living things are composed of cells •Cells are the basic unit of structure and function in living things. •New cells are produced from existing cells What type cells are there out there? Two different types Prokaryotes pronounced (pro-carry-oats) Pro = before Karyo = kernel or seed “Before the Nucleus” Prokaryotic cells are one where the genetic material is not contained in a nucleus. The DNA is just floating in the cell in a ring called a Plasmid. Best Example – E-coli bacteria in your gut EuKaryotes pronounced (you-carry-oats) Eu = true Karyo = kernel or seed “True Nucleus” Eukaryotes have genetic material stored in the nucleus. They are: •generally bigger •have organelles •may live in multi-cellular units Best example - You The Cell Structures: Eukaryotic cells of plants and animals have many structures. Animal Plant Organelle Information The following information will be given: 1. Animal or Plant 2. Structure and function 3. Relationship with other organelles 4. Interesting facts 5. Mr. Ellis’s shortened definition The Nucleus ( actually four different organelles) Found in Plant and Animal Usually not found in the center of the cell, but to the side. Ellis Def. – “Cell Brain” 4 parts 1. Chromatin DNA in the cell 2. Nucleolus Makes Ribosomes 3. Nuclear Envelope Protects the DNA 4. Nuclear Pores “door” in and out of nucleus Endoplasmic Reticulum Found in both Plant and Animal Always associated with the Nucleus 2 Types! 1. ROUGH Endoplasmic Reticulum Ellis Def. – “Protein Factory” •A long tube-like structure used to process proteins. •Looks “rough” due to riobosomes attached to the outside surface. Actual micrograph 2. SMOOTH Endoplasmic Reticulum Ellis Def. – “Fat Factory” •Lipids that are to be made by the cell are processed in the smooth E R. •Usually found near the Rough E R Actual micrograph Golgi Apparatus a.k.a.(Golgi bodies,or Golgi structures) Found in Plant and Animal Always found near the Endoplasmic Reticulum Ellis Def. – “Cell U.P.S.” •Products from the E. R. are packaged in the Golgi. •Products move to the outside edges and break out into “bubble” structures to move to other parts of the cell. Actual micrograph Camillo Golgi Vesicles Found in Plant and Animal Found all over the cell, but mainly near the Golgi Apparatus. Ellis Def. – “U.P.S. Truck” •These are the “bubble” structures that leave the Golgi. •Take products everywhere in the cell. Cellular Organization: Organelles are arranged away from the nucleus based on their function. Nucleus Rough E.R. 1. The nucleus sends out signals. 2. The signals are sent to the rough E.R. or the Smooth E.R. (depending on what is to be made). 3. Rough E.R. makes proteins, Smooth E.R. makes lipids. 4. Products move to the Golgi to be packaged. 5. Vesicles take the products to their destination. Smooth E.R. Golgi Vesicles Ribosomes (free or attached) In Plant and Animal Ellis Def.- “Protein Assembler” Found near the Rough E.R. Found free floating in the Cell. Made from two units of RNA. Attached Ribosomes Mitochondria Found everywhere in Plant and Animal Cells Ellis Def. – “Cell Powerhouse” •Produces energy for the cell. (ATP) •Made from two layered membrane. •Has its own DNA •Divides on its own schedule, not with the cell •Endosymbiotic Theory states that it was once a bacteria and is now living within the cells. Micrograph of Mitochondria Lysosome Found everywhere in Plant and Animal Cells Ellis Def. – “Pacman” •Lysosomes have enzymes •They are a specialized vesicle. •They “eat” materials the cell takes in (breaking it into usable subunits) •They can also open up and destroy the cell if there is an irresolvable problem. Cytoplasm Found everywhere in Plant and Animal Ellis Def. – “Cell Goo” •Made of over 95% water. •The rest is salts and dissolved ions. •Supports the cell shape and provides for movement of organelles. Cytoplasm Cytoskeleton Two different types Ellis Def. – “Skeleton” 1. Microtubules Small hollow tubes 2. Microfilaments Even smaller threads •Together, these two make up the scaffolding that organelles use as structure of the cell. •Used in the Centrioles and Flagella (Flagellum – singular) Centrioles Found Animal only! ONLY found in Animal cells! ONLY found by the Nuclear Membrane. Ellis Def. – “Cell Anchors” •Made of Small Tube Proteins that fuse together to make the structure’s shape. •Used by the cell to “anchor” the Chromosomes in cell division. This is a micrograph of the end of the Centriole Structure of Eukaryotic Cilia/ Flagella 9x2 + 2 ring of microtubules Covered in plasma membrane Basal Body Anchors cilia/ flagella to cell 9x3 ring of microtubules Wave Back and forth Dynein “walking” moves Cilia and Flagella •Powered by ATP •Dynein arms grip adjacent microtubule •Grip, pull, release – repeat •Results in bending of microtubules Motor Molecules & Cytoskeleton •Changing shape •Cilia movement •Flagella movement Organelles move along microtubule or microfilament Ex: Vesicle transport Microfilaments (actin filaments) Pulling force Muscle contraction Cytoplasmic streaming Pseudopodia • Part of Extracellular Matrix – Support cell membrane – Inside of cell membrane Extracellular Matrix Cell Membrane Both Plant and Animal Ellis Def. – “Cell Skin” •Made from two layers of phospholipids backed up to each other. •Proteins “float” in the membrane These two chains make what is called ”Lipid Bilayer”. { Chloroplast Found ONLY in plant cells Ellis Def. – “Sugar Mill” •Plants use these to make the sugars that they store for later use. •Contains stacks of Thylakoids upon which photosynthesis takes place (chlorophyll). The Stroma • As with the is where light energy is made into Mictochondria, they are thought to be a sugars. bacterial brought in •Similar to Mitochondria in that by Endo-symbiotic they have their own division theory schedule Cell Wall Plant Only! Ellis Def.–“Cell Wall” duh! •Rigid Starchy structure that surrounds and protects plant cells •Has small openings that allows plants to communicate with each other. Chromoplasts Found in Plants ONLY Ellis Def. – “Nature’s Color-wheel” •Pigments other than Chlorophyll within the plant. Usually only seen when Chlorophyll breaks down in the cold. •Carotenes (orange/yellow) and Xanthophylls (red/purple) •Found in leaves, fruits, and flowers. •Thought to be for attraction of pollinators and seed dispersers. Central Vacuole Ellis Definition: “Water Bag” • The Central Vacuole is used to store water for the plant. • **It also acts by providing structure to the plant. • **if full, the cell is Turgid. • **if not full, the cell is Flaccid. • Turgid cells make a healthy plant • Flaccid cell - Wilting Cell Processes Your Cell Membrane controls what comes in and what goes out of your cells. This is done through the proteins that “float” in the membrane. The basic unit of the membrane is the Phospholipid. Phosphate head Lipid Tails { The proteins that “float” in the membrane are used for specific purposes: • Some are used for signaling. They receive messages • Some are for materials movement They have openings to allow materials to pass in or out • Some are cell Identification • Some are for Regulation They move specific materials (NaCl and K) in or out. How do cells connect? Cell Junctions in Animal Cells Fuses membranes Strong sheets Pass ions and small molecules Passive Transport Active Transport 1. Simple Diffusion 2. Facilitated Diffusion 3. Osmosis 1.Active Transport 2.Phagocytosis 3.Pinocytosis 4.Exocytosis Review for Test: 1. 2. 3. 4. 5. 6. 7. 8. 9. Define the following: Robert Hooke Anton Von Leeuwenhoek Schleiden Schwann Nuclear envelope Nuclear pores Chromatin Nucleolus Smooth Endoplasmic Reticulum Answer the following: 10. Rough Endoplasmic Reticulum 11. Ribosomes 12. Lysosomes 13. Mitochondria 14. Cytoskeleton 15. Microtubules 16. Microfilaments 17. Golgi apparatus 18. Cytoplasm 19. Central Vacuole 20. Cell membrane 21. Chromoplasts 22. Cell Wall 23. Centrioles 24. Exocytosis 25. Prokaryotes 26. Eukaryotes 27. Tight Junctions 28. Desmosomes 29. Gap Junctions 1. What are the components of the cell cycle 2. Follow a protein from the DNA in the Nucleus to the end placement within or outside the cell. 3. Compare and contrast the cells of plants and Animals.