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MOLECULAR BIOLOGY of the GENE Biology 5870 FALL 2006 Dr. Alex Georgakilas Office: N 418; phone: 328-5446; e-mail: [email protected] Office hours: 11:00--12:30 PM, M-T-TH., or by appointment Lectures: ROOM 107 HOWELL SCIENCE NORTH; Time: T-TH 9:30 am- 10:45 am Texts: MOLECULAR BIOLOGY OF THE GENE 5th ED. By Watson et al. Pearson/Benjamon-Cummings. Molecular Genetics (5870) is a course designed to give emphasis on the molecular mechanisms of DNA Organization, induction of DNA Damage and Mutagenesis, DNA Repair, Replication, Transcription, Translation, Recombination, Gene Regulation and protein expression primarily in eukaryotic organisms. At the same time a brief description will be given on several current molecular biology techniques for the analysis and characterization of DNA or protein. Brief description of the chapters: Divided into five parts, the first (Chemistry and Genetics) begins with an overview of molecular biology, placing the discipline in historical context and introducing the basic chemical concepts that underpin our description of molecular biology today. The second and third parts (Maintenance of the Genome and Expression of the Genome) form the heart of the book, describing in detail the basic mechanisms of DNA Repair, replication, transcription and translation. The fourth part of the book (Regulation) deals with how gene expression is regulated - from the examination of basic mechanisms that regulate gene expression in bacterial and eukaryotic systems, to a description of how regulation of gene expression lies at the heart of the process of development. Recent findings from sequencing whole genomes of several animals have revealed that they all share essentially the same genes. The last chapter in the regulation section looks at how changes in gene regulation can account for how different animals can be made up of the same genes. The final part of the book (Methods) deals with the techniques and methods used in molecular biology. Exam Procedures & Grading Policy Grades will be based on four ~one-hour exams including the final which will not be a comprehensive. All exams count equally towards your final grade. Your lowest exam grade will be dropped and it will be replaced with your highest exam grade i.e., your highest exam will count twice. However in order to do this YOU MUST TAKE ALL EXAMS. These exams represent your only point opportunities and will cover lecture material as well as reading assignments from the texts. Exams will follow an essay question/short answer format. Students with excused absences will take a make-up exam; unexcused absences result in a 0 on the missed exam. Grades are based on a firm 10 pt. grading scale, i.e., 90-100 = A; 80-89 = B; 70-79 =C; 60-69 =D; < 60 = F. Grade rounding will be considered only in exceptional cases (59.5=60, 69.5=70, 79.5=80, 89.5=90). THERE WILL BE NO CURVING OF INDIVIDUAL EXAM SCORES. NO MAKE-UP EXAMS. Study Guides & Help Sessions Study guides, consisting of a vocabulary list, problems, & reading assignments, will be issued to aid in preparing for exams. It is to your advantage to use these along with the text while studying. The course lectures will also be listed on Blackboard. I will hold help sessions prior to each exam. The time and location of sessions will be arranged in class. Accommodations for Students with Special Needs East Carolina University seeks to comply fully with the Americans with Disabilities Act (ADA). Students requesting accommodations based on a covered disability must go to the Department for Disability Services, located in Brewster A-114, to verify the disability before any accommodations can occur. The telephone number is 252-328-6799. Academic Integrity Every student is expected to complete all assignments and activities for this course in accordance with the Principles of Academic Integrity as outlined in the Student Handbook. Actual or attempted cheating, plagiarism, or falsification are serious violations of ECU’s honor code and will be dealt with as specified by the University (see Student Handbook for details).Please do not place yourself or the instructor in the position of having to deal with these serious issues. LECTURE SCHEDULE Date Aug 24 Aug 29 Aug 31 Sep 05 Sep 07 Sep 12 Sep 14 Topic Text Introduction Ch. 1-2 Nucleid Acids as Carriers of Genetic Information Ch. 1-2 Weak and Strong Bonds Determine the DNA Structure Ch. 3-5 >> Ch. 3-5 The Structures of DNA and RNA Ch. 6 Organization of DNA in Chromosomes Ch. 7 >> Ch. 7 Sep 19 FIRST EXAM Sep 21 Sep 26 Sep 28 Sep 29 Oct 03 Oct 05 Oct 10 The Replication of DNA >> DNA Damage, Repair and Mutagenesis LAST DAY TO DROP CLASSES >> Homologous Recombination >> Oct 12 SECOND EXAM Oct 14-17 Fall Break Ch. 8 Ch. 8 Ch. 9 Ch. 9 Ch. 10 Ch. 10 Oct 19 Oct 24 Oct 26 Oct 31 Transcription/Translation Mechanisms-Genetic Code >> >> >> Nov 02 THIRD EXAM Nov 07 Nov 09 Nov 14 Nov 16 Nov 21 Gene Regulation-Emphasis in Eukaryotes >> >> >> Techniques in Molecular Biology/Applications Nov 22-26 Nov 28 Nov 30 Dec 05 Dec 07 Ch. 16-18 Ch. 16-18 Ch. 16-18 Ch. 16-18 Ch. 20 Thanksgiving Break Techniques in Molecular Biology/Applications >> Overview of all chapters Overview of all chapters Dec 14 Thursday FINAL EXAM I look forward to working with you this semester Ch. 12-15 Ch. 12-15 Ch. 12-15 Ch. 12-15 Ch. 20 Ch. 20