Download BME 5XXX Protein Engineering Course Summary

Florida International University - College of Engineering – Department of Biomedical Engineering – Miami
BME 5XXX
Protein Engineering
Catalog Description:
Protein engineering: Cloning, express and purification of proteins, and E.Coli and Yeast
expression systems. Design of proteins for specific end uses.
Course Objectives: Ability to design protein modifications to enhance thermo-, non-aqueous
stability. Understand principles of drug design based on 3d structures of drug & target protein.
Ability to access, query and employ molecular structure and sequence databases.
Acquire skills in molecular graphics modeling of small molecule and protein structures.
Appreciation of how protein engineering has contributed to understanding protein structure and
folding,
enzyme
mechanisms,
antibody-antigen
&
protein-DNA
interactions.
A realistic assessment of the future potential for protein engineering and design.
Learning Outcomes:
Student will have the ability to do following:
1. Competent Cell Culture Experiment
2. DNA Amplification Experiment – PCR (Polymerase Chain Reaction)
3. Agarose Electrophoresis Experiment
4. DNA Extraction and Purification
5. DNA Cutting and Ligation for Constructing a Plasmid Vector
6. Experiment of Heterologous DNA Transformation into Host Strains
7. Expression Experiment – Induction by IPTG and Methanol
8. Protein Analysis Experiments – SDS-PAGE Electrophoresis and Western Blotting
9. Protein Purification via FPLC
Major Topics:
1. Biophysics
2. Molecular biology
3. Biotechnology
4. Physical biochemistry
5. Biotechnology
6. Protein Structure
Prerequisites:
Permission of the instructor.
Textbook:
Molecular Biology, Robert Weaver, WCB-McGraw-Hill, New York, NY, 2003.