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BBS 3219 Advanced molecular biology and biotechnology
Course description
This course covers advanced molecular biology and principles of biotechnology. This is largely a
practical course with most of the time spent in the laboratory.
Course Objectives
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To describe some advanced concepts in molecular biology
To describe the principles of biotechnology.
Learning outcomes:
Apply advanced molecular biology techniques to disease diagnosis and research investigations..
Content outline
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Biotechnology
o Vectors
o Enzymes for nuclear research, hybridization, DNA-synthesis
o PCR
o Synthesis of primers, ligation, transfection, reverse transcription
Uses in genetics
o Gene libraries, construction and their uses
o Restriction frgment length polymophisms analysis
Genetic engneering
o Recombinant DNA technology
o Cell culture techniques
o Mutagenesis,
o Hybridisation
o Plasmids protoplasta and cell fusion
o Monoclonal antibodies
o Cloned genes
Gene transfer
o Inborn genetic defects and disease
Requirements:
4 Weeks, 60 CH
Mode of assessment
Practical examination 40%
Final summative examination 60%