Download TITLE: ELODEA CELLS 05

TITLE:
ELODEA CELLS
05
Text: page 34-42
Purpose: To identify basic structures of plant cells.
Materials:
microscope
ruler
light
water
slide
dropping bottle
coverslip
methylene blue stain
paper towels
paper
pencil
CAUTION!!!! Methylene blue stain is a very dark blue stain. It will
probably NOT wash out of your clothing. It is not harmful to your skin
but it will leave a stain. Wash off your skin with soap and water as soon as possible. Any
remaining stain on your skin will eventually wear off.
1. WEAR A LAB APRON.
2. AVOID GETTING STAIN ON CLOTHING OR SKIN.
3. KEEP THE BOTTLE OF STAIN AWAY FROM THE EDGE OF THE TABLE.
4. IF A SPILL DOES OCCUR, NOTIFY YOUR TEACHER IMMEDIATELY.
Procedure:
1. Put the clean slide on the lab table. Put a drop of water in the center of the slide.
2. Put one FRESH GREEN leaf from the elodea plant in the water on the
3. Apply the coverslip.
1.
slide.
Put the slide on the stage of the microscope and focus on the leaf at 40 X. SCAN THE SLIDE. Notice
the arrangement of the cells.
5. Increase magnification to 100 X and focus in on a small area of the leaf.
6. Now DRAW a group of cells at 100 X. IDENTIFY and LABEL the following parts:
A. cell wall - rigid outside covering on plant cells.
B. cell membrane - thin layer inside the cell wall.
C. cytoplasm - filling material inside cell.
D. chloroplasts - TINY, round green structures containing chlorophyll. Chloroplasts float in the
cytoplasm
7. Remove the slide from the microscope. Remove the coverslip.
8. Apply a small drop of stain to the leaf and let it soak in for
about two minutes, then blot off the stain with a paper towel.
9. Add a drop of water to the stained leaf and apply a coverslip.
10. Examine the slide again at low and then high power. TRY to locate
a cell with a nucleus and ADD this to your drawing. LABEL.
A. nucleus - dark blue round structure inside the cell.
11. Remove the slide from the microscope and clean it. Put all materials
away!!!! COMPLETE DRAWING, LABELING, AND CHART BEFORE QUESTIONS.
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MAKE SURE THAT YOU HAVE LABELED A TOTAL OF FIVE STRUCTURES ON YOUR DRAWING!
Questions: Answer all questions in a sentence, your own sentence - do not copy from other students; use key
words. Use text pages 34-42 for help.
1. What is the function of a cell wall?
2. What is the function of the cell membrane?
3. What is the name of the green material found in chloroplasts?
4. What is the function of a chloroplast?
WARM-UP MATCHING - PICK THE CORRECT LETTER - USE PAGES 34-42
__________1. Plant with very thin leaves;
found in ponds.
A. CELL WALL
__________2. Stiff, rigid, box-like covering
on plant cells.
__________3. Thin layer inside plant cell
wall or outside layer on
animal cell.
__________4. Rounded, dark-staining object
inside cell.
__________5. Thick, liquid filling material
found inside cell.
__________6. Tiny, green structures floating
in cytoplasm.
C. CELL MEMBRANE
05
B. CHLOROPLASTS
D. ELODEA
E. NUCLEUS
F. CYTOPLASM
ELODEA CELLS: DRAW AND LABEL 5 PARTS ON RIGHT. USE A RULER!!!!!!!!
DRAW HERE
LABEL HERE
ANSWER QUESTIONS ON BACK. USE TEXT PAGES 34-42 FOR HELP