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國立彰化師範大學九十三學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
第 1 頁，共 3 頁
1. (a) What is the absorption spectrum, action spectrum, and difference spectrum, respectively? (6 %)
(b) How does the difference spectrum along with inhibitors make a study in the respiratory chain
of inner mitochondrial membrane? (4 %)
2. (a) Give the detail procedures of analyzing the mixture solution of Asp, Arg, glu,lys, phe, and
tyr – type of column, pH buffer,sequence of elute…..。(8 %)
(b) Give the principal and application for PAGE (polyacrylamide gel electrophoresis) ,
SDS-PAGE (SDS-polyacrylamide gel electrophoresis), and gel filtration. (6 %)
3. (a) Give the significance of kcat, and kcat/KM. in enzymatic reaction. (3 %)
(b) What is R and T state, respectively? (4 %)
And how do they relate to allosteric regulation of enzymatic reaction? (3 %)
4. (a) What is isotope? (2 %)
And give 4 radioactive isotopes and 2 stable isotopes that used in biological materials. (6 %)
By what instrument you can detect these two type isotopes – radioactive and stable, respectively? (2 %)
(b) In terms of the time of exposure of the biological system to the radioisotope, what three kinds
of tracer experiments can be categorized?
And give one example for each tracer experiment. (6 %)
5. Why the circular dichroism can be used to detected the changes in protein conformation? (10%)
6. What is the “Bohr effect”? (10%)
單選題 （每題三分）
7. Which of the following is most likely to be partly associated in weak aqueous solutions?
(A) alcohol
(B) lactic acid
(C) potassium sulfate
(D) sodium chloride
(E) sodium lactate
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國立彰化師範大學九十三學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
第 2 頁，共 3 頁
8. Which of the following is a conservative substitution?
(A) Val for Ile
(B) His for Pro
(C) Asp for Ala
(D) Lys for Leu
(E) Ser for Ala
9. Serine proteases:
(A) hydrolyze peptide bonds involving the carboxyl group of serine residues.
(B) are characterized by having several active sites per molecule, each containing a serine residue.
(C) are inactivated by reacting with one molecule of diisopropylfluorophosphate per molecule
of protein.
(D) are exopeptidases.
(E) are synthesized in an active form in eukaryotes.
10. Study of the papin hydrolysis products of an antibody indicates:
(A) antibodies are bivalent.
(B) the products have decreased affinity for antigens.
(C) each antibody molecule is hydrolyzed into many small peptides.
(D) the hypervariable sequences are in the hing region of the intact molecule.
(E) none of the above is true.
11. Membrane channels:
(A) have a large aqueous area in the protein structure so are not very selective.
(B) commonly contain amphipathic alpha-helices.
(C) are opened or closed only as a result of a change in the transmembrane potential.
(D) are the same as gap junctions
(E) allow substrates to flow only form the outside to inside of the cell
12. Which of the following require(s) a transporter that specifically binds a solute?
(A) active mediated transport
(B) gap junction
(C) membrane channel
(D) simple diffusion
(E) all of the above
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國立彰化師範大學九十三學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
第 3 頁，共 3 頁
13. In glycolysis ATP synthesis is catalyzed by:
(A) hexokinase.
(B) 6-phosphofructo-1-kinases.
(C) glyceraldehydes-3-phosphate dehydrogenase.
(D) phosphoglycerate kinase.
(E) None of the above.
14. When blood glucagons rises, which of the following hepatic enzyme activities FALLS ?
(A) adenylate cyclase
(B) protein kinase
(C) 6-phosphofructo-2-kinase
(D) fructose 1,6-bisphosphatase
(E) hexokinase
15. Glycoproteins:
(A) may contain 95% or more carbohydrate.
(B) always contain a serially repeating carbohydrate unit.
(C) are found only intracellularly.
(D) from different animal species may have a different primary sequence in the protein
but have identical carbohydrate chains.
(E) have the carbohydrate linked to the protein by either N- or O-glycosidic bonds.
16. Lysine as a nutrient:
(A) maybe replaced by its alpha-keto acid analog.
(B) produces pyruvate and acetoacetyl CoA in its catabolic pathway.
(C) is methylated by S-adenosylmethionine.
(D) is the only one of the common amino acids that is a precursor of carnitine.
(E) All of the above are correct.
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