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The 1st International Conference “Animal Production and Environment”
© 2012 Can Tho University. All rights reserved.
Changes in muscle cell cation regulation and meat quality
traits are associated with genetic selection for high body
weight and meat yield in broiler chickens
Dale Sandercock1,2, Zoe Barker1,2,3, Malcolm Mitchell1,4, Paul Hocking1,2, *
1
Department of Agricultural Systems, Mekong Delta Development Research Institute, Can Tho University
Postal address: 3/2 street, Xuan Khanh ward, Ninh Kieu District, 92100 Can Tho city, Viet Nam
Email: [email protected], Tel.:+84-710 3832475, Fax: +84-7103 831270
Veterinary staff, People’s Committee of Phuoc Hao Commune, Chau Thanh district, Tra Vinh province
Postal address: People’s Committee of Phuoc Hao Commune, Chau Thanh district, Tra Vinh province, Vietnam
Email: [email protected], Tel.: +84-7403 890284
2
3
Department of Animal Sciences, College of Agriculture and Applied Biology, Can Tho University
Postal address: 3/2 street, Xuan Khanh ward, Ninh Kieu District, 92100 Can Tho city, Viet Nam
Email: [email protected], Tel.:+84-710 3872081, Fax: +84-7103 830814
Abstract (not exceed 200 words)
C8 is a component of the membrane attack complex (MAC) of the complement system,
which causes lysis of the target cells. C8 consists of three subunits C8A, C8B, and C8G.
This study focuses on the porcine C8G gene (pC8G) aiming to identify its cDNA sequence,
to detect single nucleotide polymorphisms (SNPs) within the gene, and to analyze its
polymorphic association with hemolytic complement activity in the classical (CH50) and the
alternative (AH50) pathway. Actually, the pC8G is 840 bp in length encoding 202 amino
acids. One SNP was detected at nucleotide 423C>T (SNP c.423C>T; codon
124GAC>GAT). Hemolytic complement activity of animals of the DUMI populations
obtained at about 6, 14 and 16 weeks of age before and after vaccinations showed shorttermed increments due to the immune stimulation and long-term increase due to aging.
Family based association tests indicated effects of C8G on AH50 and CH50 at 16 weeks of
age immediately before PRRS vaccination (AH50_PRRS0 p=0.087; CH50_PRRS0
P=0.027). However, the results did not indicate a consistent effect of the respective alleles
on hemolytic complement activity in both pathways. This study provides weak evidence for
the candidacy of the pC8G for innate immune response and disease resistance in pigs.
Keywords: innate immunity, association, complement system
Introduction
References in the text should follow the Harvard system in style, i.e. name(s) followed by
date: Miller (2010), Brown & Smith (1999), Jones et al. (2009), Jones et al. (2009a, 2009b).
Unpublished data must be cited as (unpublished data).
Materials and methods
Animals
Samples of animals of the different pig breeds Hampshire (HS, n=1), Duroc (DR, n=1),
German Landrace (LR, n=30), Pietrain (PI, n=30), Berlin Miniature pig (BMP, n=1), and
Muong Khuong (MK, n=25) were used to sequence cDNA and to screen for SNPs.
Experimental design
....
Results and discussion
2
Table 1: Genotypic and allelic frequencies in several pig populations
German Landrace
Pietrain
Muong Khuong
Genotypes
n
%
n
%
n
%
CC
0
0.00
0
0.00
21
0.84
CT
0
0.00
0
0.00
3
0.12
TT
30
1.00
30
1.00
1
0.04
Figure 1. A representative pattern for PCR result of 118 DNA
clones of the IMpRH. The symbols (0), (?), and (1) used for
absent, ambigous and present results, respectively. The letters a,
b, c, and d show a hamster sample (nagative control), a blank
sample without DNA (negative control), a pig DNA (positive
control) and a marker to estimate length of DNA fragments.
Conclusion
Main text here
Acknowlegement
main text here
References
Full articles
Schroeder W, Stock KF, Distl O (2010) Genetic evaluation of Hanoverian warmbloo d horses for conformation
traits considering the proportion of genes of foreign breeds. Arch Tierz 53: 377-387.
Sandercock DA, Barker ZE, Mitchell MA, Hocking PM (2009) Changes in muscle cell cation regulation and meat
quality traits are associated with genetic selection for high body weight and meat yield in broiler chickens. Genet
Sel Evol 41:8.
Full articles (not in English)
Batura J, Korzienowski W, Bochno R (1990) [Effect of duck limited feeding on fatty acid composition of depot and
muscle fats]. Prze Nauk Lit Zoot 17: 133 -140 [in Polish].
Book chapters
Chilliard YJ, Rouel A, Ferlay L, Bernard P, Gaborit K, Raynal -Ljutovac AL, Leroux C (1990) Optimising goat milk
and cheese fatty acid composition: effects of genotype, feeding factors and dairy technology. In: Williams C,
Buttriss J (eds.) Improving the fat content of foods. Woodhead Publishing, Cambridge, UK: 281-312.
Conference abstracts
Galina M, Haenlein GFW (2004) Cheese quality and human health. Proc. 8th Intern Conf Goats, Pretoria, South
Africa: 42.