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Ch. 7 Cellular Structure and Function I. Cell discovery and Theory Main Idea: the invention of the microscope led to the discovery of cells. A. History of the Cell Theory 1. the invention of the microscope led to the discovery of cells a. Robert Hooke (1665): English monk/scientist used a simple microscope to view a piece of cork which reminded him of monk’s “cells” or rooms in a monastery; he was actually looking at dead cell walls. b. Anton van Leeuwenhoek: Dutch scientist who designed his own microscope and first saw microorganisms. B. Cell Theory: one of the fundamental principles of modern Biology; 3 principles: 1) All living things are made of cells. 2) Cells are the basic unit of structure and function in any organism. 3) All cells come from preexisting cells. C. Basic Cell Types 1. ALL cells have: a. plasma membrane that acts as a boundary and helps control what enters and leaves the cell b. genetic material that provides instructions for making substances that the cell needs. c. Break down molecules for energy. 2. Cell categories: a. Eukaryotic cells: contain membrane-bound organelles (specialized structures that carry out specific cell functions) and a nucleus (central organelle that contains cell’s genetic material in the form of DNA). i. Most organisms are made of eukaryotic cells and are called “eukaryotes”; algae and yeast are unicellular eukaryotes b. Prokaryotic cells: cells without a nucleus or other membrane-bound organelles. i. “prokaryotes”: most unicellular organisms like bacteria; probably similar to the first life on Earth. II. The Plasma Membrane Main Idea: The plasma membrane helps to maintain a cell’s homeostasis. A. Function of the Plasma Membrane: the plasma membrane helps to maintain a cell’s homeostasis. 1. plasma membrane is a thin, flexible layer b/w the cell and its environment that allows nutrients into the cell and allows wastes and other products to leave the cell. 2. selective permeability: property that allows some substances to pass through the membrane while keeping others out. B. Structure of the Plasma Membrane 1. phospholipid bilayer: makes up the plasma membrane where two layers of phospholipids are arranged tail-to-tail. a. Phospholipids look like a round head with two tails b. Phosphate is the polar head and is attracted to water c. The two fatty acid tails are nonpolar and are repelled by water d. The two layers create a barrier that is polar on the surface and nonpolar in the middle that prevents water-soluble substances from easily moving through the membrane. 2. other components: a. receptors: proteins on the membrane surface that send signals to the inside of the cell b. transport proteins: move needed substances or waste materials through the plasma membrane. c. Cholesterol: positioned among the fatty acid tails and prevents them from sticking together d. Carbohydrates: stick out from the membrane and help identify chemical signals and define a cell’s characteristics. 3. fluid mosaic model: concept of the plasma membrane that describes how the phospholipids and other components are in constant motion within the membrane creating a pattern on the surface. III. Structures and Organelles Main Idea: Eukaryotic cells contain organelles that allow the specialization and the separation of functions within the cell. A. Cytoplasm and Cytoskeleton 1. cytoplasm: the semi-fluid material inside the plasma membrane a. in prokaryotes, all chemical processes take place directly in the cytoplasm b. in eukaryotes, chemical processes take place within organelles in the cytoplasm 2. cytoskeleton: network of long, thin protein fibers that form a framework for the cell and provide an anchor for the organelles inside. a. Microtubules: long, hollow protein cylinders that form a rigid skeleton for the cell and assist in moving substances. b. Microfilaments: thin protein threads that give the cell shape and enable the cell to move. B. Cell Structures (organelles): allow different chemical processes to take place at the same time in different parts of the cytoplasm. 1. nucleus: contains cell’s DNA which stores information to make proteins for cell growth, function and reproduction a. nuclear envelope: double membrane that surrounds the nucleus b. chromatin: complex DNA attached to protein spread throughout the nucleus 2. ribosomes: manufacture proteins a. made of RNA and a protein b. not bound by a membrane c. made by nucleolus d. some free-float in cytoplasm/ others are bound the endoplasmic reticulum 3. endoplasmic reticulum (ER): membrane system of folded sacs and channels that serves as the site for protein and lipid synthesis a. rough ER: areas where ribosomes are attached for protein synthesis b. smooth ER: areas where no ribosomes are present; provides surface for synthesis of carbohydrates and lipids 4. Golgi apparatus: flattened stack of membranes that modifies, sorts and packages proteins into sacs called vesicles. These vesicles fuse with the plasma membrane to release proteins to the environment outside the cell. 5. vacuoles: membrane-bound vesicles that can temporarily store materials (food, enzymes) within the cytoplasm. a. animal cells usually do not contain vacuoles 6. Lysosomes: vesicles that contain substances that digest excess or worn-out organelles and food particles. a. Protect cell by digesting bacteria and viruses that enter the cell. 7. Centrioles: groups of microtubules that function during cell division a. Located in the cytoplasm in animal cells near the nucleus. 8. Mitochondria: converts sugars into usable energy a. Has highly folded inner membrane that provides a surface for breaking bonds in sugar molecules b. “powerhouses” of the cell 9. chloroplasts: capture light energy and convert it to chemical energy (photosynthesis) a. present in plant and other eukaryotic cells b. thykaloids: disk-shaped compartments containing chlorophyll that trap solar energy. c. Plastid: group of organelles used for storage (starch, lipids) i. Chromoplasts: red (chromophyll), orange or yellow (xanthophyll) pigments that trap energy and give color to flowers and leaves 10.cell wall: thick, rigid mesh of fibers that surrounds the outside of the plasma membrane in plant cells, protecting and supporting it. a. Made of cellulose (carbohydrate) 11.cilia and flagella: microtubules that project outside the plasma membrane that allow movement. a. Cilia are short, flagella are long and whiplike C. Comparing cells 1. Plant cells: cell walls, chlorophyll with chloroplasts, large vacuoles 2. Animal cells: no or small vacuoles present, lysosomes IV. Cellular Transport Main Idea: Cellular transport moves substances within the cell and moves substances into and out of the cell. A. Diffusion: the movement of particles from an area of high concentration of a substance to an area of lower concentration of the substance. 1. occurs b/c particles in gases, liquids and solids are in constant random motion (Brownian motion) 2. dynamic equilibrium: state reached where particles continue to move but no change in concentration occurs. 3. rate of diffusion is affected by concentration, temperature and pressure B. Diffusion across the plasma membrane: although water can diffuse across the plasma membrane, the cell needs other substances like chloride ions and sugars to perform cell functions that can’t easily diffuse through the plasma membrane. 1. facilitated diffusion: occurs when transport proteins move ions and small molecules across the plasma membrane. Requires no additional energy (passive transport) since particles are moving from area of high concentration to low concentration. a. channel protein: transport protein that allows substances to move into the cell by opening and closing. b. Carrier protein: transport protein that changes shape to help move particles across the plasma membrane C. Osmosis: the diffusion of water across a selectively permeable. 1. water will move across to balance out the concentration of a solute on both sides of a selectively permeable membrane. 2. isotonic solution: occurs when a cell is in a solution that has the same concentration of water and solutes (ions, sugars, proteins) as in its cytoplasm. a. The cell is at equilibrium with the solution and there is no net movement of water in or out of the cell, so it retains its shape. 3. hypotonic solution: occurs when a cell is in a solution that has a lower concentration of solute than the cell’s cytoplasm. a. Water moves into the cell and creates osmotic pressure that causes animal cells to swell or even burst if the pressure is too great. b. Cell walls prevent plant cells from bursting in a hypotonic solution; the vacuole fills with water pushing the cell membrane against the cell wall causing the plant cell to become firmer. 4. hypertonic solution: occurs when a cell is placed in a solution with higher concentration of solute than the cell’s cytoplasm. a. water moves out of the cell and causes animal cells to shrink b. plant cells lose water from central vacuole causing the cell membrane to shrink away from the cell wall which causes the plant to wilt. D. Active Transport: the transport of substances from an area of lower concentration to higher across the plasma membrane: requires energy 1. carrier proteins called pumps can move only one substance in only one direction, while others move two substances either across the membrane in the same direction or in opposite directions. a. Na+/K+ATPase pump: found in plasma membrane of animal cells i. Maintains levels of sodium and potassium ions inside and outside the cell. ii. Pump uses ATP (energy molecule) to transport three sodium ions outside the cell while moving two potassium into the cell. iii. Coupled transport: sodium atoms pumped outside the cell couple with sugar molecules and are transported into the cell through a membrane protein called a coupled channel through facilitated diffusion. E. Transport of Large Particles 1. endocytosis: cell surrounds an outside substance with its plasma membrane, pinches off and leaves the substance inside the cell in a vacuole. 2. exocytosis: reverse of endocytosis; cell expels wastes and secrete substances (like hormones) outside the cell Both #1 and #2 require energy.