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3rd Benchmark Biology Review Name_______________________ Goal 3.04: Chapter 13 and 14 Genetic Engineering and The Human Genome Project 1. The Human Genome Project is an attempt to SEQUENCE HUMAN DNA. One of the goals is to help people with genetic disorders by replacing a faulty gene; this is calledGENE THERAPY 2. Selective breeding: 2 types are HYBRIDIZATION, INBREEDING and they produce desired TRAITS/CHARACTERSTICS. 3. Two sources of genetic variation are MUTATIONS, and SEXUAL reproduction. In sexual reproduction(meiosis), these two processes:_TETRAD FORMATION and CROSSING OVER, guarantee variation. Inducing mutations increases VARIATIONSin populations. 4. Polyploidy, as in strawberry plants, results in a new plant species because it increases the number of CHROMOSOMES, and makes the plant HEARTIER /LARGER-STRONGER. 5. This figure shows a RESTRICTION ENZYME cutting a DNA strand, which is one of the first steps in producing RECOMBINANT DNA. Figure 13–1 6. A gene that makes it possible to distinguish bacteria that carry a recombinant DNA plasmid from those that don’t carry the new DNA is called a(an)GENETIC MARKER and Antibiotics resistant to genes resistant genes are often used. This has been causing the evolution of new bacteria with RESISTANT GENES 7. The process of making changes in the DNA code of a living organism is called GENETIC ENGINEERING 8. 1. 2. 3. Analyzing DNA by gel electrophoresis allows researchers to: COMPARE GENES OF DIFFERENT ORGANISMS (Species) COMPARE GENES OF DIFFERENT INDIVIDUAL ORGANISMS LOCATE & IDENTIFY ONE PARTICULAR GENE (of thousands that an individual has) 9. Band D is further from the sample WELLS. This is because band D consists of SHORTER / SMALLER fragments of DNA which are SMALLER / LIGHTER than band C fragments. The process being shown below is GEL ELECTROPHORESIS 10.Stem cells are UNSPECIALIZED cells (have no assigned TISSUE) that can be used to repair damaged NERVE, MUSCLE, & LIVER cells. There is significant controversy over stem cells because scientists can get them from EMBRYOS. 11.An advantage of cloning is that it produces IDENTICAL organisms/ tissues and can be used to REPLACE diseased organs and produce consistently high yield CROPS like corn. It is a form of ASEXUAL reproduction. 12.Two advantages of producing transgenic plants:contain genes that produce: 1) natural insecticides, 2) resist weed killing chemicals, 3) resist rot & food spoilage, 4) may produce human antibodies. Another name for these plants is GENETICALLY MODIFIED ORGANISMS or GMO’s. Two problems with producing transgenic plants are LOSS OF NATURAL NUTRIENTS and LOSS OF NATURAL HORMONES & ANTIBODIES may spread.(roundup ready corn is hard to get rid of in a neighbor’s field, pest-resistant corn can kill beneficial insects) 13. Label the steps scientist take to produce transgenic bacteria that make insulin: Goal 3.05 Examine the Theory of Evolution by Natural Selection 14.Summary of Darwin’s 5 points to Natural Selection a. b. c. d. e. 1. 2. 4. 5. 15. There is natural variation in populations. These naturally occurring traits are inherited, not acquired characteristics like ______________________. 16. Describe the 6 lines of evidence for evolution and give an example for each 1. ________________ ____________- DNA, proteins, and ______ acids are compared to determine common ancestry of organisms. If few differences, it is a more _______ common __________. 2. _________________- embryos of vertebrates are similar, grow similar tissue 3. _________________- ________________________________ 4. homologous structures-________________________________ 5. _________________-________________________________ 6. _________________-_______________________________ 17. Match the theory with the experiment and sketch in the margin: a. BIOGENESIS b. ABIOGENESIS c. ENDOSYMBIOTIC THEORY gooseneck flask of broth let air in, but not bacteria________ covered jar of meat did not let flies in, so only uncovered jar had maggots_______ abiotic stew and lightning could produce organic molecules_______ eukaryotes evolved from aerobic and photosynthetic prokaryotes which either invaded or were engulfed by other prokaryotes. 6. The _____________Theory states that ___________ bacteria gave rise to mitochondria, and _________ bacteria was the first chloroplast. They developed into the first ____________cell. 18. Evolution is a _________ in ____________frequency. 19. Speciation is the __________________________________. Reproductive isolation can cause speciation by a. __________________________-_____________________________ b. __________________________- ____________________________ 20. Define 4 methods of evolution, and give an example. 1. adaptive radiation- _____________________________________________________ 2. ______________ - _____________________________________________________ 3. ______________- _____________________________________________________ 4. coevolution______________________________________________________ 19. Relative Dating Absolute Dating 20. List the pattern of evolution for a. dophins and man _____________________ b. dolphins and sharks____________________ c. insects and flowers_____________________ 21. Sketch the resulting population curve below if a. average sized beaks were preferred by females b. small seeds and large seeds survived the drought c. Large beaks did better, and the avg size increased A. B. C. Goal 4.01: Analyze the classification of organisms according to their evolutionary relationships 22. Linneaus Classification System had just ___kingdoms. Linneaus used ________________ relationships;we now use __________________relationships using bio__________evidence. With more knowledge of diversity of organisms, Modern System has __Domains and ___ Kingdoms: Kingdom Domain a. __________________-_____________________________________________ b. __________________-_____________________________________________ c. __________________-_____________________________________________ d. __________________-_____________________________________________ e. __________________-_____________________________________________ f. __________________-_____________________________________________ 23. cell structure: Animal Plant euk/ cell wall Fungi Protist Eubacteria Archaebacteria some autotroph some heterotroph asexual (binary fission) get energy reproduction multi except uni or multicells: 24. Cell size Organelles Chromosomes ______ Eukaryotes Prokaryotes 25. The acronym we use for the taxons are :____________________________________________. 26. The letters stand for ____________, which are divided into ____________, divided into ______________divided into ____________, divided into _____________ divided into _____________ divided into______________. 27. Binomial _________________is used to assign scientific names and are made up of the ____________ and ________, which are in Latin and are _______________ 28. Closely related species will have a more recent common ancestor than others. This is reflected in sharing the same_________. They will have fewer differences between their _______ acid sequences in their ________________. This is ___________________evidence for evolutionary relationships. 29. The three domains are _______________which include animals, protists and ___________, Eubacteria which includes _______________, and ___________________, made up of methanogens, halophiles and thermophiles. Bacteria are now broken up into two domains and kingdoms because of differences in ___________________________________________.