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Transcript
Investigating Cell Migration Under
Microgravity Conditions
Co-Principal Investigators:
Sylvia Edoigiawerie, Kamsi Odinammadu, Amelia Smith, Beverly Wu
Teacher Facilitator:
Dr. Michelle Starz-Gaiano
University of Maryland, Baltimore County
University System of Maryland, Maryland
2016 SSEP National Conference
Smithsonian Air and Space Museum, Washington, DC
June 29-30, 2016
Cell Migration is Essential
(if you are in the animal kingdom)
Human Embryo
Development
www.ehd.org
http://www.primobaby.com
Immune Cell Migration
Wound
Healing
and Immune
Response
http://makeagif.com/I0Z2VD
http://en.wikipedia.org/wiki/File:Laceration,_leg.jpg
Overview: The Process of Cell
Movement
● Actin (cytoskeletal
protein) → facilitates movement
● E-cadherin (membrane
protein) → involved in cell to cell
adhesion
Integrative Model of Cell Migration
Actin
Activating protein
(cytoskeleton)
E-Cadherin
(adhesion)
Cell locomotion
Cells in the Fly Ovary are a Model
for Migration
Advantages: fantastic genetic tools
small transparent tissues
border cells
blue= nurse cell nuclei
ovariole (L. Manning)
Does gravity affect the number of cells that become
motile or their ability to complete movements?
Activator
Experimental Plan
http://swampthingscience.com/?author=1
Flies
engineered to
make migratory
cells fluoresce
green
www.hansoltc.com
1 sex per
FME
section
with fly
food –
open to
allow
mating
www.e-reagent.com
Use
chemical
fixative to
freeze
cells in
place
https://www.thinglink.com
Observe
and score
ovaries
Analysis and Interpretation
microgravity
predictions
Disorganized F-Actin?
F-Actin
Lathienna Manning, Starz-Gaiano
microgravity
predictions
E-cadherin
Niewiadomska, P., D. Godt, and U. Tepass.
Disrupted or decreased
E-cadherin?
(normally actin binding
stabilizes E-cadherin)
Future Directions
Grounds for more research on the impact of
microgravity on immunity
● A focus on astronaut health in space conditions grows in
importance as space exploration expands
Acknowledgements
NCESSE
Starz-Gaiano Laboratory
University of Maryland, College Park
University of Maryland, Baltimore
County
Maryland Space Grant Consortium
Department of Cell Biology and
Molecular Genetics, UMD CP
The Drosophila Community for Fly
Stocks
Bloomington Stock Center
Funding for the Starz-Gaiano lab from
National Science Foundation