Download An Excel Macro to Visualise Patterns for Chosen Genes

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Transcript
My Excel Macro
Based on macro by Frank Millenaar (Utrecht)
Selects genes of interest from Mas5 output spreadsheet
Visualises expression profiles as combined:
data-tables
heat-maps
Can adapt for different combinations of chips to give different profiles
Eg a range of organs.
Similar in function to GenevestigATor
….but email me if you want to have a go.
[email protected]
Input
Unformatted NASCAffy (MAS5) output
– All 23,000 genes from ATH1 chip
– For each gene
•
•
•
•
•
•
•
SpotID
Probe Name
Gene Name
Description
Process
URL
For each array
– Signal value
– Detection Call
– Detection P Value
• Each replicate chip is separate (3) columns
– IE to much data for a human mind to see patterns!
List of genes of interest
output
Data for our chosen genes only
Can use different chip sets
– e.g. organs
– e.g. treatments
genes
Replicate chips can be
meaned
• Gene Name
• For each chip
– Signal value
chips
For each gene, only:
Signal values
colour-coded
for visual impact
= Heat map
annotation
• (Description)
• (Process)