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SUMMARY • Sudden Oak Death – Deemed introduced because disease was never seen before, mortality rates were very high, and distribution did not match range of hosts – Genetic studies reveal simple genetic structure in forests. Only one lineage of clonally reproducing individuals. AFLPs and microsatellites indicate forest lineage is different from european nursery lineage. In US nurseries three very different lineages are found – Symptoms vary depending on host: in oaks the pathogen causes a girdling necrosis with bleeding on the trunk, on rhodies and bays it cause leaf blight – Epidemiologically important vs. dead-end hosts •US forest isolates clearly distinct from EU nursery isolates, also have different mating type •Isolates from nurseries in WA, OR, & BC both of the US and EU types •Potential for XXX sex and recombination in US nurseries •US forest population is genetically very homogeneous, trademark of an introduced species The entire genome was sequenced in less than 3 years since discovery of organism * 12 SSR loci (di- and tri- repeats identified) * Loci selected to be polymorphic both between and within continental populations * 500+ representative isolates analyzed CCGAAATCGGACCTTGAGTGCGGAGAGAGAGAGAGACTGTACGAGCCCGAGTCTCGCAT We found same genotypes in nurseries and forests proving origin of wild outbreak Bay/Oak association Bay Coast Live Oak (no sporulation) Canker margin in phloem Bleeding canker Sporangia Infectious diseases spread not randomly but around initial infections Duglas-fir sapling branch tip wilted by P. ramorum Symptoms on Buckeye leaves and petioles Scorching of maple leaves caused by P. ramorum Spring Autumn More problems • Host lists started expanding ( now over 100) in all plant families and ferns • Symptoms looked extremely different on different hosts • Isolation of organism from symptomatic tissue often not possible • Isolation success extremely different in different seasons Environmental sample DNA extraction Dna probes (plus/minus) ++- DNA fingerprints DNA-based diagnostics Designed 2 sets of P. ramorum specific primers (www primer3 software) • phyto1-phyto4 (1st round PCR) • highly specific for P. ramorum • 687 bp fragment (in between red arrows) • phyto2-phyto3 (2nd round PCR) • nested in phyto1-4 amplicon; specific for Phytophthora spp. • 291 bp fragment (in between yellow arrows) ITS1 Phyto1 5.8S ITS2 Phyto2 Phyto3 Phyto4 Fraction Positive Culture versus nested PCR 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 PCR Pos Culture Pos Total (N=216) Foliar: Foliar: Bay Wood Not Bay (N=36) (N=65) (N=116) Significant effect of diagnostic type (P <0.001) and sample type (P=0.0036) The assay we developed became the first DNA assay to diagnose non viral plant pathogens. Now diagnosis of most microbes will be DNA based Synchrony pathogen-host Spores in rainwater 2001-06 600 250 500 Disease cycle 200 SE + 400 150 300 100 200 50 l ct n r l ct n r l ct n r l ct n r l ct n r0 l Ju O Ja Ap Ju O Ja Ap Ju O Ja Ap Ju O Ja Ap Ju O Ja Ap Ju 2002 2003 month 2004 2005 Total precipitation (mm) Mean cfu/L 0 100 80 60 Davidson et al. 2005; unpublished Susceptibility of oaks (lesion size) 40 20 0 Mar-03 May-03 Jul-03 Sep-03 Nov-03 Jan-04 Mar-04 Average lesion (mm_) 80 60 40 Wetness > 12 h 20 0 0 6 12 18 24 30 36 42 48 54 Time (h) 2 Lesion area (mm) 50 40 30 Temp >19 C 20 10 0 15 17 19 21 23 25 Temperature ( C) 27 29 Bay Laurel / Tanoak SOD Spore Survey 35 Temp (C) 30 Rain (mm) 25 20 15 10 5 0 Date How to control emergent exotic diseases • PREVENT THEIR INTRODUCTION • LIMIT THE HUMAN-SPREAD OF PATHOGENS (infected plants, plant parts, dirty tools) • EMPLOY HOST RESISTANCE • CHEMICAL AND OTHER MITIGATION STRATEGIES Forest pathogens can never be eradicated PREVENT: Diagnose Symptoms relatively generic, very variable, and pathogen not always culturable DNA TESTS LAB CULTURES AgriFos and PentraBark Topical Application + Agrifos vs. Azomite Treatments (efficacy 1 - 24 months) a 14 a Canker Size (mm) 12 10 8 6 b 4 2 0 Azomite Positive Control Agrifos New host pathogen combinations • Pathogen stays/Plant moves: invasive plant • Pathogen moves/Plant stays: exotic epidemic • Pathogen moves/Plant moves: biological control Success. The “1:10” rule • Can exotic withstand new environment • Can it withstand attacks of predators • Can it outcompete similar native organisms by accessing resources – Can a pathogen be pathogenic – Can a pathogen be sufficiently virulent • Invasion driven by ecological conditions • Enemy release hypothesis • Resource availability (pathogenicity/virulence) Pathogenicity • Qualitative: ability to cause disease • Often regulated by a single gene • Avr genes in pathogen and resistance genes in host Gene for gene • Resistance in host is dominant • Virulence is recessive ar aR Ar AR Gene for gene • Resistance in host is dominant • Virulence is recessive ar aR Ar AR Resistance: no disease Functions of avr/R genes • Avr genes may help detoxify plant enzymes, secure necessary aminoacids or proteins, plant toxins, promoting pathogen growth. Normally they are mobile, wall-bound products • R genes normally recognize multiple avr genes and start hypersensitive response (programmed cell death) Avr/R genes matches are specific • Race of the pathogen (avr1) matched by variety of the crop (R1). • At the base of crop breeding science • If R genes target avr genes linked to important housekeeping functions, they are more durable Can be R genes accumulated? • There is a cost associated with R genes • Mostly R genes initiate costly defense processed, often even when challenged by innocuous microbes • Some evidence that in absence of specific avr, R are lost Plants immune response • Plants do not possess an immune system such as that of animals • They do recognize pathogens • Recognition initiates secondary metabolic processes that produce chemicals that will stop or slow microbial infections: thickening of cell wall, premature cell death (HR response), systemic resistance Virulence: quantitative response • Multiple genes controlling: – Phenotypic traits conferring virulence – Production of plant detoxifying enzymes – Production of plant toxins CAN WE PREDICT: • Success of an exotic microbe? – Survival structures such as cysts, spores, etc – Saprotrophic ability (ability to feed on dead matter) – Degree of host specialization, the more specialized the harder it may be to establish – Phylogenetic distance of hosts (the closertive and new hosts are, the easier the establishment) – Similar ecology CAN WE PREDICT: • Levels of the epidemic? – Density dependance: abundance of susceptible hosts – Genetic variation in host. In general it is assumed that genetic variation in host populations slows down epidemics, however backing data from natural ecosystems is missing. It could be that low genetic diversity associated with widespread presence of resistance may be more beneficial than genetic variability CAN WE PREDICT: • Selection of increased R in host? – Host: R to exotic may be significantly present because it identifies native pathogen. – R may be absent. – R may be present at low frequency. If host does not exchange genes long distance, but only in areas already infested there is a stronger selection process. Otherwise locally selected R genes may be swamped by genes coming from outside the area of infestation – Shorter generation times favor pathogen The red queen hypothesis • Coevolutionary arm race • Dependent on: – Generation time has a direct effect on rates of evolutionary change – Genetic variability available – Rates of outcrossing (Hardy-weinberg equilibrium) – Metapopulation structure CAN WE PREDICT: • Selection of increased virulence in pathogen? – It depends on the presence or absence of trade-off – Does increased virulence make pathogen more fit? – It has been shown that in some cases (but not always), there is a trade-off between virulence and transmission • Rapid generation time of pathogens. Reticulated evolution very likely. Pathogens will be selected for INCREASED virulence if no trade-offs are present • In the short/medium term with long lived trees a pathogen is likely to increase its virulence • In long term, selection pressure should result in widespread resistance among the host Frequency-, or density dependent, or balancing selection • New alleles, if beneficial because linked to a trait linked to fitness e.g. by conferring advantageous heterozygosity will be positively selected for. – Example: two races of pathogen are present, but only one resistant host variety, suggests second pathogen race has arrived recently – Mating alleles: two mating alleles indicate a single founder individual and high relatedness among genotypes. In a varied natural population you expect multiple mating alleles