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Plant Diseases Forensic Diagnostic Methods Andres S. Espindola Dept. Entomology and Plant Pathology Oklahoma State University Plant Diagnostics: Importance Plant diseases spread as quick as human diseases Infected plants can not be saved. Quick plant pathogen diagnosis Quick on site diagnosis Specialized lab The United States crops are a billionaire business Signs and Symptoms At a glance, take a look in signs and symptoms. Signs: Presence of the pathogen (bacterial cells) Signs and symptoms may give a guidance to plant pathologist. Pseudomonas syringae pv. tomato Symptoms: responses of the plant. Different techniques for Diagnosis Symptoms and signs are not the only tool to diagnostic a plant disease. Immunological diagnostic tests Human Immune System Plant pathogen proteins injection Positive Result Negative Result Invalid Results Pictures of real Immunostrips Agglutination Antibodies Bacteria (Plant pathogen) The results are going to be similar to this: Agglutination: How does it happen? Agglutination: How does it happen?