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Lecture 6 Outline (Ch. 9) I. Overview of Respiration II. Redox Reactions III. Steps of Respiration IV. Cellular Respiration A. Glycolysis B. Coenzyme Junction C. Citric Acid Cycle (aka Krebs/TCA cycle) D. Electron Transport Chain (ETC) E. Chemiosmosis V. Anaerobic respiration VI. Respiration using other biomolecules VII. Lecture Concepts Cellular Respiration Overall purpose: • convert fuels to energy • animals AND plants • complementary to photosynthesis Cellular Respiration Cellular Respiration: (Exergonic) • catabolizes sugars to CO2 • requires O2 • at mitochondrion Redox Reactions • as part of chemical reaction, e- are transferred • e- transfer = basis of REDOX reactions (reduction) (oxidation) Redox Reactions • follow the H, e- w/them Redox Reactions Equation for respiration Redox Reactions • transfer of e- to oxygen is stepwise Redox Reactions • e- moved by NAD+ (niacin) • when “carrying” e- (& H+), NADH • gained e- (& H+), reduced Where do e- come from? • food (glucose) Where do e- go? • glucose NADH ETC O2 (H2O) Steps of Respiration • Steps of respiration: 1. glycolysis - cytosol Coenzyme Junction 2. Citric acid cycle - mitochondrial matrix 3. ETC - inner mitochondrial membrane 4. Chemiosmosis - inner membrane to intermembrane space Cellular Respiration • Stages of respiration: 1. Glycolysis – prep carbons Cellular Respiration 1. Glycolysis • 1 glucose (6C) 2 pyruvate (3C) • key points: - inputs - ATP - NAD+/NADH - CO2 and H2O - outputs • eukaryotes AND prokaryotes Glycolysis Cellular Respiration -inputs: 1 Glucose 2 ATP -outputs: 2 pyruvate 4 ATP (2 net) 2 NADH CO2 none 2 H2O Where do they go? Cellular Respiration Coenzyme Junction • 2 pyruvate (3C) • pyruvate joins coenzyme A (vitamin B) • 2 C lost (as CO2) • 2 NAD+ NADH 2 Acetyl CoA (2C) Steps of Respiration • Stages of respiration: 2. Citric acid cycle e- transfer: redox Cellular Respiration 2. Citric acid cycle • few ATP so far • mitochondrial matrix • 2 Acetyl CoA (2C) join oxaloacetate (4C) • 2 citrate (6C) converted several steps, 4C lost (CO2) • e- to carriers (NAD+, FAD) Citric acid cycle -inputs: 2 Acetyl CoA (2C) -outputs: [2 oxaloacetate (4C)] 2 ATP 6 NADH 2 FADH2 4 CO2 H2O none Where do they go? Self-Check Step of Respiration Inputs Outputs CO2/H2O ATP produced e- carriers loaded Glycolysis 1 glucose 2 pyruvate 2H2O 2 net 2 NADH Coenzyme Junction Citric Acid Cycle Electron Transport Chain Oxidative phosphorylation Fermentation Steps of Respiration • Steps of respiration: 1. glycolysis - cytosol Coenzyme Junction 2. Citric acid cycle - mitochondrial matrix 3. ETC - inner mitochondrial membrane 4. Chemiosmosis - inner membrane to intermembrane space Steps of Respiration • Stages of respiration: 3. ETC Proton Motive Force Substrate-level phosphorylation Phosphate group moved from substrate to ADP yields ATP Cellular Respiration 3. Electron transport chain (ETC) • lots of energy harvested • released in stages • so far, 4 ATP – substrate P • many ATP – oxidative phosphorylation Cellular Respiration – mitochondria revisited Cellular Respiration Electron transport chain (ETC) • ETC e- collection molecules • embedded on inner mitochondrial membrane • accept e- in turn • e- ultimately accepted by O2 (O2 reduced to H2O) Electron transport chain (ETC) -inputs: per glucose, 10 NADH 2 FADH2 10 H+ -outputs: ATP (none yet) ~100 H+ (stored) 10 H2O Where do they go? H+ build up in intermembrane space (potential energy) Steps of Respiration • Stages of respiration: 4. Chemiosmosis ATP produced! Cellular Respiration 4. Chemiosmosis • ATP synthase: inner mitochondrial membrane • energy input ATP – H+ gradient • chemiosmosis – ion gradient to do work Cellular Respiration 4. Chemiosmosis • Four parts to ATP synthase: Rotor, Stator, Rod, Knob • H+ must enter matrix here • Generates 1 ATP per ~3.4 H+ Cellular Respiration Summary of respiration • Cells convert ~ 40% of energy in glucose to energy in ATP • Most fuel efficient cars convert only ~ 25% of gasoline energy Cellular Respiration - anaerobic • no O2 – no oxidative phosphorylation • fermentation • extension of glycolysis • substrate-level phosphorylation only • need to regenerate e- carrier (NAD+) Cellular Respiration - anaerobic • Types of fermentation 1. alcohol • pyruvate converted to acetaldehyde • acetaldehyde accepts e• ethanol produced Cellular Respiration - anaerobic • Types of fermentation 2. Lactic acid • pyruvate accepts e- • lactate produced Cellular Respiration - anaerobic • inputs/outputs • alcohol • pyruvate in • CO2 and EtOH out • brewing & baking • lactic acid • pyruvate in • lactate out • muscle fatigue Cellular Respiration • pyruvate - junction • O2 present – citric acid cycle • O2 absent fermentation Self-Check • Comparison of aerobic vs. anaerobic respiration: Aerobic • ATP made by: • ATP per glucose: • initial eacceptor: • final eacceptor: Anaerobic Cellular Respiration – other biomolecules • Glucose catabolism – one option • Proteins: Catabolized into a.a. Amino group removed – Enter diff. points – depends on a.a. • Fats: Glycerol in at glycolysis - becomes pyruvate Fatty acids in before CAC - becomes Acetyl CoA Self-Check Step of Respiration Inputs Outputs CO2/H2O ATP produced e- carriers loaded Glycolysis 1 glucose 2 pyruvate 2H2O 2 net 2 NADH Coenzyme Junction Citric Acid Cycle Electron Transport Chain Oxidative phosphorylation Fermentation Lecture 6 concepts - Describe in words the purpose of cellular respiration - Write the equation for cellular respiration - Given an equation, particularly that for cellular respiration, determine which molecules are oxidized and reduced - List the steps of cellular respiration and where they occur - For glycolysis, coenzyme junction, and the citric acid cycle, give inputs, outputs, ATP made/used, e- carriers loaded, water and carbon dioxide molecules produced - For the electron transport chain and chemiosmosis, give inputs, outputs, ATP made/used, e- carriers loaded, water and carbon dioxide molecules produced - Keep track of the total number of carbon molecules going in and coming out for each step of respiration - Compare substrate-level and oxidative phosphorylation - Compare the e- carriers, final e- acceptors, and ATP made for aerobic respiration and anaerobic respiration - Write out a list of new terminology and provide descriptions