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ALL LIFE IS CELLULAR! (The rest is just details.) A View of the Cell Images Types What do they do? What about cells? Quiz History & Microscopes Organelles Membrane Leaf Cells #1 Bacteria #2 Cell Images Blood Cells Striated Muscle #3 Back To images Cytoplasmic streaming Back to images What am I seeing? NUCLEUS Back To images To what am I seeing? What am I seeing? These are onion cells. The “boxes” are the actual cells What is the dark round structure inside the cells? What are the “stringy” structures in some cells? Go Back and click on these to find out. Back to previous Back to images What Is This? Various blood cells What kind of blood cell is this? It is a Red Blood Cell Also, known as an Erythrocyte Their function is to transport oxygen throughout the blood. What kind of blood cell is this? It is a type of White Blood Cell. White blood cells are known as Leukocytes. There are many kinds of WBC’s. They work within the immune system to fight invaders. What are some types of invaders? Back to images Back To images How Big Is it? Actually, this is not big at all. It is a bacterial cell and is about 1/10,000th the size of the other cells you have viewed. Are bacteria cells the same as all other types of cells? How are bacterial cells classified? Are all bacteria harmful? Back to menu Characteristics of Bacteria Cells They are Prokaryotic Cells not Eukaryotic Cells This means they do not have membrane covered nuclei or organelles inside. The DNA is in a “region” of the cell, but they do have ribosomes which don’t have a membrane. They have a cell wall but not made of cellulose like plant cell walls. It is made of peptidoglycan – a combination of protein and carbohydrates. All bacteria belong to Kingdom Monera and all Monerans are prokaryotes and all prokaryotes are Monerans. Classifying Bacteria Shape Spherical - Cocci / Coccus Example: Streptococcus pyrogenes Rod like - Bacilli / Bacillus Example: Bacillus anthracis Spiral - Spiralli / Spirallis Example: Vibrio cholerae Gram Staining Test Technique Yuk! Strep throat! Light microscope image What is this? The 3 types of muscle cells: Skeletal cardiac smooth Can you see the striped appearance of these cells? “Striated” means striped – this is striated skeletal muscle The stripes are a result of light and dark bands within individual muscle cells called “sarcomeres” The striated muscle fibers are made up of actin and myosin (proteins that can contract/shorten) which meet like zipper teeth. A muscle contracts (shortens) when the myosin and actin elements are pulled together along their length. Back to Muscle slide Back to leaf Light microscope image w/ Gram stain Electron microscope image •Determines the type of cell wall a bacteria has •Slides are washed successively in the following dyes: crystal violet, iodine, alcohol, safranin •Gram positive organisms are purple (or bluish) •Gram negative organisms are red •Helpful in determining treatment for bacterial infections – which medicine should you take? Gram Negative Gram Positive Two major types of cells Prokaryotes Cells without true nucleus; not surrounded by a membrane Has DNA in a nucleoid region No membrane-bound organelles Has ribosomes Has a cell wall – not made of cellulose like plant cell walls; Made of peptidoglycan – a combination of protein and carbohydrate Only bacteria are prokaryotic cells PROKARYOTES Eukaryotes Cells with a true nucleus(DNA) surrounded by a membrane DNA in a nucleus Organelles covered in a membrane Plants Animals Fungi One celled Protists Amoeba Algae Euglena paramecium EUKARYOTES Plant and Animal Cells Two Basic Cell Types Cells that do not contain internal membrane-bound structures are called prokaryotic cells. Click here • The cells of unicellular bacteria do not have membrane bound structures and are therefore called prokaryotes. PROKARYOTIC CELLS EUKARYOTIC CELLS PLANT CELLS ANIMAL CELLS The Life of a Cell A View of the Cell A cell is the basic unit of life. Back to menu The History of the Cell Theory • Before microscopes were invented, people believed that diseases were caused by curses and supernatural spirits. • As scientists began using microscopes, they quickly realized they were entering a new world–one of microorganisms. • Microscopes enabled scientists to view and study cells, the basic units of living organisms. Development of Light Microscopes The first person to record looking at water under a microscope was Anton van Leeuwenhoek. The microscope van Leeuwenhoek used is considered a simple light microscope because it contained one lens and used natural light to view objects. Development of Light Microscopes Compound light microscopes use a series of lenses to magnify objects in steps. These microscopes can magnify objects up to 1 500 times. The Cell Theory • Robert Hooke was an English scientist who lived at the same time as van Leeuwenhock. • Hooke used a compound light microscope to study cork, the dead cells of oak bark. Cells are the basic building blocks of all living things. Back to menu More Info here The cell theory is made up of three main ideas: All organisms are composed of one or more cells. The cell is the basic unit of organization of organisms. All cells come from preexisting cells. Back to menu Electron microscopes Development of Electron Microscopes The electron microscope was invented in the 1940s. This microscope uses a beam of electrons to magnify structures up to 500 000 times their actual size. Development of Electron Microscopes There are two basic types of electron microscopes. The scanning electron microscope scans the surface of cells to learn their three dimensional shape. The transmission electron microscope allows scientists to study the structures contained within a cell. A prokaryotic cell does not have internal organelles surrounded by a membrane. Most of a prokaryote’s metabolism takes place in the cytoplasm. 2. DNA • Ribosomes (the dots) 6. Cytoplasm 3. Plasma membrane 4. Cell wall 5. Flagellum This eukaryotic cell from an animal has distinct membrane-bound organelles that allow different parts of the cell to perform different functions. 1. Nucleus 2. Nucleolus 3. DNA/Chromosomes 4. Plasma membrane 5. Other Organelles The membrane-bound structures within eukaryotic cells are called organelles. Each organelle has a specific function that contributes to cell survival. • Separation of organelles into distinct compartments benefits the eukaryotic cells. The nucleus is the central membranebound organelle that manages cellular functions. The Plasma Membrane PLANT CELLS ANIMAL CELLS Cellular Boundaries The plasma membrane acts as a selectively permeable membrane. The cell wall The cell wall is a fairly rigid structure located outside the plasma membrane that provides additional support and protection. Nucleus and cell control Chromatin Nuclear Envelope Nucleolus Assembly, Transport, and Storage The endoplasmic reticulum (ER) is an organelle that is suspended in the cytoplasm and is the site of cellular chemical reactions. Assembly, Transport, and Storage Endoplasmic Reticulum (ER) Ribosomes Assembly, Transport, and Storage Golgi Apparatus Vacuoles and storage Vacuoles are membrane-bound spaces used for temporary storage of materials. Notice the difference between vacuoles in plant and animal cells. Animal Cell Vacuole Plant Cell Lysosomes and recycling Lysosomes are organelles that contain digestive enzymes. They digest excess or worn out organelles, food particles, and engulfed viruses or bacteria. Energy Transformers: Chloroplasts and energy Chloroplasts are cell organelles that capture light energy and produce food to store for a later time. Chloroplasts and energy The chloroplasts belongs to a group of plant organelles called plastids, which are used for storage. Chloroplasts contain green pigment called chlorophyll. Chlorophyll traps light energy and gives leaves and stems their green color. Mitochondria and energy Mitochondria are membrane-bound organelles in plant and animal cells that transform energy for the cell. Mitochondria and energy A mitochondria, like the endoplasmic reticulum, has a highly folded inner membrane. Energy storing molecules are produced on inner folds. Structures for Support and Locomotion Cells have a support structure called the cytoskeleton within the cytoplasm. The cytoskeleton is composed of microtubules and microfilaments. Microtubules are thin, hollow cylinders made of protein and microfilaments are thin solid protein fibers. Cilia and flagella Some cell surfaces have cilia and flagella, which are structures that aid in locomotion or feeding. Cilia and flagella can be distinguished by their structure and by the nature of their action. Cilia and flagella Cilia are short, numerous, hair-like projections that move in a wavelike motion. Cilia Cilia and flagella Flagella Flagella are long projections that move in a whip-like motion. Flagella and cilia are the major means of locomotion in unicellular organisms. All living cells must maintain a balance regardless of internal and external conditions. Survival depends on the cell’s ability to maintain the proper conditions within itself. Why cells must control materials The plasma membrane is the boundary between the cell and its environment. It is the plasma membrane’s job to: • allow a steady supply of glucose, amino acids, and lipids to come into the cell no matter what the external conditions are. • remove excess amounts of these nutrients when levels get so high that they are harmful. • allow waste and other products to leave the cell. This process of maintaining the cell’s environment is called homeostasis. Selective permeability is a process used to maintain homeostasis in which the plasma membrane allows some molecules into the cell while keeping others out. Plasma Membrane Water Structure of the Plasma Membrane The plasma membrane is composed of two layers of phospholipids back-to-back. Phospholipids are lipids with a phosphate attached to them. The lipids in a plasma membrane have a glycerol backbone, two fatty acid chains, and a phosphate group. Phosphate Group Glycerol Backbone Two Fatty Acid Chains Makeup of the phospholipid bilayer The phosphate group is critical for the formation and function of the plasma membrane. Phosphate Group Makeup of the phospholipid bilayer The fluid mosaic model describes the plasma membrane as a flexible boundary of a cell. The phospholipids move within the membrane. The Plasma Membrane Other components of the plasma membrane: Cholesterol plays the important role of preventing the fatty acid chains of the phospholipids from sticking together. Cholesterol Molecule Other components of the plasma membrane: Transport proteins allow needed substances or waste materials to move through the plasma membrane. What happens in cells? Control of cell functions Photosynthesis Protein, lipid, carb. production Digestion Contraction Secretion Energy transformations Protection Support Information transfer Detect information others There are many kinds of cells All are specialized to do different things Not all cells perform all functions Cells are very specialized 1.How did the invention of the microscope impact society’s understanding of disease? A. Scientists were able to view microorganisms that were previously unknown. B. Microscopes were invented after the development of the cell theory. C. It was once believed that viruses, not bacteria, caused diseases. D. Scientists could view membrane-bound organelles of prokaryotes. The answer is A. Before microscopes were invented, people believed that curses and supernatural spirits caused diseases. Microscopes enabled scientists to view cells, which led to the discovery that microorganisms cause some diseases. 2. Which of the following uses a beam of light and a series of lenses to magnify objects in steps? A. compound light microscope B. scanning electron microscope C. transmission electron microscope D. simple light microscope The answer is A. Most microscopes use at least two convex lenses. Compound light microscopes use a light beam and a series of lenses and can magnify objects up to about 1500 times. Electron microscopes use a beam of electrons and can magnify structures up to 500 000 times. 3. What makes this cell eukaryotic? A. Because it has a cell wall. B. Because it contains DNA. Nucleus Nucleolus C. Because it Chromosomes has membranebound organelles. D. Because it does not have DNA. Plasma membrane Organelles The Answer is C. Eukaryotic cells contain membrane-bound organelles that have specific functions in the cell; prokaryotic cells do not. Nucleus Nucleolus Chromosomes Plasma membrane Organelles 4. Describe the structure of the plasma membrane. The plasma membrane is composed of a phospholipid bilayer, which has two layers of phospholipids back-to-back. The polar heads of phospholipid molecules contain phosphate groups and face outward. Phospholipid molecule 5. Why is the phosphate Polar head group of a (includes phospholipid phosphate important to the Nonpolar group) plasma tails (fatty membrane? acids) When phospholipid Phospholipid molecule molecules form a bilayer, the phosphate Polar head groups lie to the (includes outside. Because phosphate Nonpolar phosphate groups are tails (fatty group) polar, they allow the acids) cell membrane to interact with its watery (polar) environments inside and outside the cell. 6. Explain why the model of the plasma membrane is called the fluid mosaic model. It is fluid because the phospholipid molecules move within the membrane. Proteins in the membrane that move among the phospholipids create the mosaic pattern. 7. What is the primary function of the cell wall? A. act as selectively permeable membrane B. provide support C. control activity of organelles D. acquire nutrients from environment The answer is B. The cell wall is an inflexible, porous barrier that provides support but does not select which molecules can enter the cell. 8. Describe the control center of a prokaryotic cell. Ribosomes DNA Plasma membrane Cell wall Prokaryotic cells do not have true nuclei; their DNA is not separated from the rest of the cell by a membrane. Plasma Ribosomes DNA membrane Cell wall 9. Which of the following structures is the site of protein synthesis? A. Golgi apparatus B. Ribosome C. Vacuole D. Lysosome The answer is B. Ribosomes are the sites where the cell produces proteins according to the directions of DNA. They can be attached to the surface of the endoplasmic reticulum or float freely in the cytoplasm. 10. What is the advantage of having numerous folds in the ER? A. It enables the ER to lie snugly against the nucleolus. B. It can create more vesicles in a smaller space. C. It can capture more light energy with more folds. D. A large amount of work can be done in a small space. The answer is D. The ER is arranged in a series of folded membranes, which, if spread out, would take up tremendous space. 11. What could you predict about a plant cell that contains fewer chloroplasts than other plant cells? A. It contains less chlorophyll. B. It contains a greater number of plastids. C. It will have an increased rate of light energy capture. D. It will appear darker green in color. The answer is A. Chloroplasts are among the plant organelles known as plastids and contain the green pigment chlorophyll. Chlorophyll traps light energy from the Sun and gives leaves and stems their green color. 12. A mutation results in the inner membranes of a liver cell’s mitochondria being smooth, rather than folded. Which of the following would you expect? A. more efficient storage of cellular energy B. it can create more vesicles in a smaller space C. decreased energy available to the cell D. fewer ribosomes available for protein synthesis The answer is C. Mitochondria transform energy for the cell. A highly folded inner membrane provides a greater surface area for producing energystoring molecules. 12. Which of the following is a main idea of the cell theory? A. All organisms are composed of one cell. B. The organelle is the basic unit structure and organization of organisms. C. All cells come from two parent cells. D. All cells come from preexisting cells. The answer is D. The cell theory states that a cell divides to form two identical cells. 13. In what type of cell would you find chlorophyll? A. prokaryote B. animal C. plant D. fungus The answer is C. Chlorophyll is the green pigment found in the chloroplasts of plant cells. 14. Which of these structures packs proteins into membrane-bound structures? A. B. C. D. Answer C depicts the Golgi apparatus, which sorts proteins and packs them into vesicles. Cell wall 15. What is the difference between the cell wall and the plasma membrane? Inside cell Outside cell Plasma membrane The plasma membrane is a flexible boundary between the cell and its environment that controls the supply of nutrients, waste, and other products entering and leaving the cell. The cell wall is a rigid structure found in plant cells, fungi, bacteria and some protists that provides support and protection but does not select which molecules can enter or leave the cell. Cell wall Inside cell Outside cell Plasma membrane 16. Which of the following organelles is not bound by a membrane? A. ribosome B. Golgi apparatus C. vacuole D. lysosome The answer is A. Ribosomes are simple structures made of RNA and protein and are not bound by membranes. Ribosomes 17. Explain the importance of cholesterol to the plasma membrane. Phospholipid molecules Cholesterol molecule Cholesterol helps to stabilize the phospholipids in the plasma membrane by preventing their fatty acid tails from sticking together. Phospholipid molecules Cholesterol molecule 18. In which of the following pairs are the terms related? A. cell wall – selective permeability B. prokaryote – mitochondria C. microfilaments – locomotion D. plastid – storage The answer is D. Plastids are plant organelles that are used for storage. 19.Which of the following structures is found in both plant and animal cells? A. chloroplast B. cell wall C. mitochondrion D. thylakoid membrane The answer is C. Mitochondria are the organelles in both plant and animal cells that transform energy for the cell. 20. __________ span the entire plasma membrane and regulate which molecules enter and leave the cell. A. Transport proteins B. Cholesterol molecules C. Ribosomes D. Microtubules The answer is A. Transport proteins form the selectively permeable membrane and move needed substances or waste materials through the plasma membrane. 21. Compare the cytoskeleton of a cell to the skeleton of the human body. The cytoskeleton and skeleton are similar in that both form a framework. However, the cytoskeleton is a constantly changing structure with the ability to be disassembled in one place and reassembled in another. ? 2 ?1 ? ?3 Back to images The History of the Cell Theory Anton van Leeuwenhoek - first person to record observing with a microscope; 1600’s Looked at water organisms •Robert Hooke- 1600’s; used a microscope to study cork; termed the word “cell”; •Matthias Schleiden -1830’s; All plants made of cells •Theodor Schwann – 1830’s; All animals made of cells •Rudolph Virchow – all cells come from preexisting cells •Rene Dutrochet- Cell is the basic functional unit of life