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Cell Transport The Need For Security • The cell membrane controls what may enter or exit the cell. • It is semi-permeable – only certain substances may pass into, or out of, the cell. • Not just anyone gets to hang with us McLovin! Passive vs. Active Transport • Passive transport does not require the use of ATP. • Active transport does require the energy of ATP. • What factors decide which method of cell transport will be employed? – Size of molecules – big or little? – The concentration gradient – with or against it? – Charge on molecules? Passive Transport - DIFFUSION • Diffusion is the movement of molecules from an area of higher concentration to an area of lower concentration. • The movement continues in this high to low manner until the concentration in both areas is the same – this is equilibrium. • Temperature and pressure can also affect the rate of diffsuion. Passive – SIMPLE DIFFUSION PASSIVE - OSMOSIS • Osmosis is the movement of water from an area of higher concentration to an area of lower concentration across a semi-permeable membrane. • Water moves because a solute within the cell is imbalanced with what is in the etracellular fluid. • The water moves in order to push out or bring together the internal solute particles in an effort to match the concentration of the external solute particles. OSMOSIS – Isotonic Conditions • If the solute concentration in the cell and its external solution is the same – the conditions are said to be isotonic. • Water still moves into and out of the cell but does so in a way that there is no net change in the concentration • One water molecule moves in while another water molecule exits the cell. OSMOSIS – Isotonic Conditions OSMOSIS – Hypertonic Conditions OSMOSIS – Hypotonic Conditions PASSIVE – Facilitated Diffusion • Facilitated diffusion uses transmembrane proteins as channels to transport ions or polar molecules across the membrane from high to low. • The proteins used here are called transport proteins and there are two types: • Channel Proteins – A hydrophilic pathway that allows water and ions to pass through it. • Carrier Proteins – A protein that binds to a molecule and transports it across the phospholipid bilayer. PASSIVE – FACILITATED DIFFUSION http://www.youtube.com/watch?v=W52PYXXPknU PASSIVE TRANSPORT ACTIVE TRANSPORT • Active transport invovles the use of ATP. There are several methods of active transport including: • Protein Pumps • Primary & Secondary Transport • • • • Phagocytosis Pinocytosis Receptor-Mediated Endocytosis Exocytosis with Vesicles ACTIVE – PROTEIN PUMPS • Primary Transport – The protein pumps substances against the concentration gradient with the use of ATP. Energy (ATP) is consumed in the process. • Secondary Transport – The protein pumps substances against the concentration gradient using the energy of an electrostatic gradient created by a method of primary transport. PRIMARY TRANSPORT SECONDARY TRANSPORT ACTIVE – PROTEIN PUMPS http://www.youtube.com/watch?v=JGF6ry0SWPs ACTIVE - PHAGOCYTOSIS • Phagocytosis means “process of cell eating”. • The cell recignizes the vital molecule or foreign invader. Then, extensions of the cell memebrane called pseudopods reach out and engulf the nutrient or neighbouring cell and place it in a (phagocytic) vesicle. • We see this in unicellular protists and white blood cells. ACTIVE – PHAGOCYTOSIS http://www.youtube.com/watch?v=6-N1cFQ0yJI ACTIVE - PINOCYTOSIS • Pinocytosis means “process of cell drinking”. This process used to take in smaller dissolved nutrients. • The cell invaginates (indents) and takes in a small portion of the extracellular fluid and the dissolved molecules within it. • This fluid and solute is placed in a (pinocytic) vesicle. ACTIVE - PINOCYTOSIS ACTIVE - RECEPTOR-MEDIATED ENDOCYTOSIS • The cell membrane has proteins on its outer surface that can be used to identify cell surface proteins on a nearby cell or protien based hormones. • The shape of receptor protein and target protein are like a key and lock. • When several receptor proteins have their target proteins attached, they move along the fluid membrane of the cell to a common point. • Now the cell will carry out a process much like pinocytosis and encapsulate the target protein molecules and receptors in a vesicle. ACTIVE - RECEPTOR-MEDIATED ENDOCYTOSIS ENDOCYTOSIS METHODS ACTIVE - EXOCYTOSIS • Exocytosis is the process of materials being moved from the cytoplasm, inside the cell, to the extracellular fluid, outside of the cell. • We often see this process undertaken with proteins made by the Rough ER. They are sent to the Golgi, packaged in a secretory vesicle and sent to the cell membrane where they are released into the extracellular fluid or blood. ACTIVE - EXOCYTOSIS & VESICLES http://www.youtube.com/watch?v=Ptzp_xZSYlM ENDOCYTOSIS & EXOCYTOSIS http://www.youtube.com/watch?v=qpw2p1x9Cic FIN