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Parts of the Cell 4.4 Eukaryotic cells are partitioned into functional compartments • There are four life processes in eukaryotic cells that depend upon structures and organelles – – – – Manufacturing Breakdown of molecules Energy processing Structural support, movement, and communication Copyright © 2009 Pearson Education, Inc. 4.4 Eukaryotic cells are partitioned into functional compartments • Manufacturing involves the nucleus, ribosomes, endoplasmic reticulum, and Golgi apparatus Copyright © 2009 Pearson Education, Inc. 4.4 Eukaryotic cells are partitioned into functional compartments • Breakdown of molecules involves lysosomes, vacuoles, and peroxisomes – Breakdown of an internalized bacterium by a phagocytic cell would involve all of these Copyright © 2009 Pearson Education, Inc. 4.4 Eukaryotic cells are partitioned into functional compartments • Energy processing involves mitochondria in animal cells and chloroplasts in plant cells Copyright © 2009 Pearson Education, Inc. 4.4 Eukaryotic cells are partitioned into functional compartments • Structural support, movement, and communication involve the cytoskeleton, plasma membrane, and cell wall Copyright © 2009 Pearson Education, Inc. Pili Nucleoid Ribosomes Plasma membrane Bacterial chromosome Cell wall Capsule Flagella NUCLEUS: Nuclear envelope Smooth endoplasmic reticulum Chromosomes Nucleolus Rough endoplasmic reticulum Lysosome Centriole Ribosomes Peroxisome CYTOSKELETON: Microtubule Intermediate filament Microfilament Golgi apparatus Plasma membrane Mitochondrion NUCLEUS: Rough endoplasmic reticulum Nuclear envelope Chromosome Ribosomes Nucleolus Smooth endoplasmic reticulum Golgi apparatus CYTOSKELETON: Central vacuole Microtubule Chloroplast Cell wall Intermediate filament Plasmodesmata Microfilament Mitochondrion Peroxisome Plasma membrane Cell wall of adjacent cell Hydrophilic head Phosphate group Symbol Hydrophobic tails Outside cell Hydrophilic heads Hydrophobic region of protein Hydrophobic tails Inside cell Proteins Hydrophilic region of protein Nucleus Double-layer membrane with pores. Connected to ER. Ribosomes made in a part called the nucleolus. Two membranes of nuclear envelope Nucleus Nucleolus Chromatin Pore Endoplasmic reticulum Ribosomes 4.9 The endoplasmic reticulum is a biosynthetic factory • There are two kinds of endoplasmic reticulum— smooth and rough • Smooth ER lacks attached ribosomes • Rough ER lines the outer surface of membranes – They differ in structure and function – However, they are connected Copyright © 2009 Pearson Education, Inc. Nuclear envelope Ribosomes Smooth ER Rough ER 4.9 The endoplasmic reticulum is a biosynthetic factory • Smooth ER is involved in a variety of diverse metabolic processes (ex: synthesis of many types of lipids) Copyright © 2009 Pearson Education, Inc. 4.9 The endoplasmic reticulum is a biosynthetic factory • Rough ER makes additional membrane for itself and proteins destined for secretion (these proteins are transported in vesicles) Copyright © 2009 Pearson Education, Inc. Transport vesicle buds off 4 Ribosome Secretory protein inside transport vesicle 3 Sugar chain 1 2 Glycoprotein Polypeptide Rough ER Golgi Receives proteins in vesicles from the ER. Modifies proteins- adds sugar chains to “mark” them for a certain destination. Puts the proteins back into vesicles and sends them out. 4.11 Lysosomes are digestive compartments within a cell • A lysosome is a membranous sac containing digestive enzymes – The enzymes and membrane are produced by the ER and transferred to the Golgi apparatus for processing – The membrane serves to safely isolate these potent enzymes from the rest of the cell Copyright © 2009 Pearson Education, Inc. 4.11 Lysosomes are digestive compartments within a cell • One of the several functions of lysosomes is to remove or recycle damaged parts of a cell – The damaged organelle is first enclosed in a membrane vesicle – Then a lysosome fuses with the vesicle, breaking down the damaged organelle Animation: Lysosome Formation Copyright © 2009 Pearson Education, Inc. Vacuoles • Functions: storage, maintaining water balance, holding pigments, etc. • Membrane-bound. Chloroplast Nucleus Central vacuole Nucleus Contractile vacuoles Mitochondria • Convert sugar (glucose) into ATP (adenosine triphosphate)- small energy packets. This is called cellular respiration. • Have two membranes (inner and outer) Mitochondrion Outer membrane Intermembrane space Inner membrane Cristae Matrix Chloroplasts • Use the sun’s energy to create glucose from carbon dioxide and water (photosynthesis) Chloroplast Stroma Inner and outer membranes Granum Intermembrane space Mitochondrion Engulfing of photosynthetic prokaryote Some cells Engulfing of aerobic prokaryote Chloroplast Host cell Mitochondrion Host cell 4.17 The cell’s internal skeleton helps organize its structure and activities • The cytoskeleton is composed of three kinds of fibers – Microfilaments (actin filaments) support the cell’s shape and are involved in motility – Intermediate filaments reinforce cell shape and anchor organelles – Microtubules (made of tubulin) shape the cell and act as tracks for motor protein Copyright © 2009 Pearson Education, Inc. ATP Vesicle Receptor for motor protein Motor protein (ATP powered) Microtubule of cytoskeleton (a) Microtubule (b) Vesicles 0.25 µm