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Transcript
Videomicroscopic study of cell motility and proliferation in vitro - Biological physics in cell biology Time-lapse imaging is a valuable tool for cell biologists to describe the dynamics of cell behaviour. A powerful computer-aided videomicroscopy system and data analysis procedure as well as characterization of motility and proliferation of cultured cells in a variety of biological systems will be presented. I. Cell cultures of human malignant brain tumors with uniform histopathological grading and proliferative capacity displayed distinct exponential velocity distribution and characteristic average velocities. II. This tumor cell cultures were also used to model the effect of therapeutic irradiation in vitro. Dose-dependent cell enlargement and inhibition of cell proliferation was found within one week following a single irradiation. A controversial motility enhancing effect of sublethal radiation was observed. III. Increased motility, path searching activity and intensified dynamism of processes were measured in primary cultures of Müller glia cells in response to the extracellular matrix molecule laminin-1. These findings indicate that statistical evaluation of physical parameters of cell behaviour obtained from in vitro videomicroscopic experiments can provide both physiologically or developmentally relevant informations and additional tools for tumour diagnosis and for testing different therapeutic modalities. Preliminary results about the measurement of cell-adhesion and viscoelastic properties of three dimensional cell aggregates formed by a series of brain tumor cell lines and tether pulling experiments using magnetic tweezers will also be presented.