Download Protein Synthesis – Level 1

Protein Synthesis – Level 1
Use the following DNA sequence to answer the questions that follow:
TACGGTAAATCGTGGATC
1. What will be the mRNA that results from transcription?
AUGCCAUUUAGCACCUAG
2. How many codons does this Mrna have?
6 codons
3. What anticodons will the tRNAs have for this mRNA? (Remember, there
is no tRNA anticodon for a stop codon)
UAC – GGU – AAA – UCG – UGG
4. What amino acids will make up the polypeptide?
METHIONINE – PROLINE – PHENYLALANINE – SERINE - THREONINE
If a mutation occurred and the DNA became:
TAGGTAAATCGTGGATC
5. What type of mutation is this?
DELETION (FRAMESHIFT)
6. How will the protein be affected (be specific).
There is no start codon, therefore the protein will not be made at all.
Protein Synthesis – Level 2
Use the following DNA sequence to answer the questions that follow:
TACGCCGTAAATCGTGGTAACGCCATC
1. What will be the mRNA that results from transcription?
AUGCGGCAUUUAGCACCAUUGCGGUAG
2. If the underlined portions represent introns, what will the mature mRNA
be/read?
AUGCAUGCAUUGCGGUAG
3. How many codons does this mature mRNA have? How many tRNA
anticodons will there be?
6 Codons
4. What anticodons will the tRNAs have for this mRNA?
UAC – GUA – CGU – AAC – GCC
5. What amino acids will make up the polypeptide?
METHIONINE – HISTIDINE – ALANINE – LEUCINE - ARGININE
If a mutation occurred and the DNA became:
TACGCCGTAAATCGAGGTAACGCCATC
6. What type of mutation is this?
Substitution (point)
7. How will the protein be affected (be specific).
The 3rd codon will change from GCA to GCU. However, both of these
codons code for the amino acid alanine, therefore, there will be no
change to the protein.
Protein Synthesis – Level 3
Use the following DNA sequence to answer the questions that follow:
3’-GCCTATACGCCGTAAATCGTGGTAACGCTATC -5’
1. What will be the mRNA that results from transcription?
5’-CGGAUAUGCGGCAUUUAGCACCAUUGCGAUAG-3’
2. If the underlined portions represent introns, what will the mature mRNA
be/read?
5’-CGGAUAUGCAUGCAUUGCGAUAG-3’
3. Prior to leaving the nucleus, what will be added to the mature mRNA?
What will the mRNA look like after this occurs? What is the purpose of
this processing?
The 5’ end will get a “cap” and the 3’ end will get a poly-A tail
(AAAAAAA). These will help prevent the mRNA from degrading too
quickly in the cytoplasm.
4. Where (what triplet) will the mRNA bind to the ribosome?
AUG
5. What amino acids will make up the polypeptide?
METHIONINE – HISTIDINE – ALANINE – LEUCINE - ARGININE
If a mutation occurred and the DNA became:
3’-GCCTATACGGCCGTAAATCGAGGTAACGCTATC-5’
6. What type of mutation is this?
INSERTION (FRAMESHIFT)
7. How will the protein be affected (be specific).
Beginning with the second codon, which changes from CAU to CCA, the amino
acids will be different, causing the protein to be non-functional. The second
amino acid will change from histidine to proline, the 3rd will change from alanine
to cysteine, etc.