Download Self Funded Research Opportunities Form Project Title : The role of

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia, lookup

Short interspersed nuclear elements (SINEs) wikipedia, lookup

DNA barcoding wikipedia, lookup

Genetic engineering wikipedia, lookup

Koinophilia wikipedia, lookup

Gene nomenclature wikipedia, lookup

Gene expression programming wikipedia, lookup

Transposable element wikipedia, lookup

Non-coding DNA wikipedia, lookup

Human genome wikipedia, lookup

Gene wikipedia, lookup

History of genetic engineering wikipedia, lookup

Therapeutic gene modulation wikipedia, lookup

Genome (book) wikipedia, lookup

Gene desert wikipedia, lookup

RNA-Seq wikipedia, lookup

Gene expression profiling wikipedia, lookup

Metagenomics wikipedia, lookup

Genomics wikipedia, lookup

Public health genomics wikipedia, lookup

Designer baby wikipedia, lookup

Site-specific recombinase technology wikipedia, lookup

Genome editing wikipedia, lookup

Artificial gene synthesis wikipedia, lookup

Minimal genome wikipedia, lookup

Genome evolution wikipedia, lookup

Pathogenomics wikipedia, lookup

Helitron (biology) wikipedia, lookup

Microevolution wikipedia, lookup

Transcript
Self Funded Research Opportunities Form
a. MSc
Project Title :
Supervisor/s:
The role of Minimal Mobile Elements in species and
strain variation in the Neisseria.
b. PhD
Degree Type :
MSc
Dr. Lori Snyder
Start & End date
Expiry date
Project Summary
This M.Sc. project will expand upon my previous investigations into Minimal Mobile Elements in the Neisseria
spp, to include Neisseria meningitidis, Neisseria gonorrhoeae, and commensal species such as Neisseria
lactamica. Minimal Mobile Elements play a role in horizontal gene transfer and integration through homologous
recombination between conserved protein-encoding genes that flank exchangeable gene cassettes.
40 different MME sites have been identified in Neisseria (Saunders and Snyder, Microbiol, 2002; Snyder et al.,
BMC Genomics, 2004; Snyder et al., Plasmid, 2005; Snyder and Saunders, BMC Genomics, 2006; Bentley et al.,
PLoS Genetics, 2007; Snyder et al., Mol Biol Evol, 2007). There is demonstrated variation in the gene cassettes
present at each site between different species and strains.
No studies have yet addressed the phenotypic differences that may be attributed to gene cassette exchange in
the Minimal Mobile Elements as a whole. The types of genes present and combinations of genes present will be
investigated, making use of the increasing amount of genome sequence data available for these bacteria.
This study will involve whole genome sequence analysis and comparative genome sequence analysis to identify
Minimal Mobile Elements. Many of the sequences to be analyzed will be incomplete and unannotated, therefore
the student must have the patience and prokaryotic genetics knowledge to correctly annotate sequences and
identify previously undiscovered Minimal Mobile Elements. Laboratory-based aspects of the project will involve
functional studies of the gene cassettes believed to contribute to phenotypic differences between species and
strains.
A passionate interest in microbiology, genomics, and molecular genetics is required. Student will be expected to
produce written pieces of work of publication quality.
Relevant references:
The repertoire of minimal mobile elements in the Neisseria species and evidence that these are involved in
horizontal gene transfer in other bacteria. Snyder LA, McGowan S, Rogers M, Duro E, O'Farrell E, Saunders NJ.
Mol Biol Evol. 2007 Dec;24(12):2802-15.
The minimal mobile element. Saunders NJ, Snyder LA. Microbiology. 2002 Dec;148(Pt 12):3756-60.
For more information please contact Dr Lori Snyder on [email protected]
Faculty of Science / Self Funded Research Opportunities Form
Page 1