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Cellular Respiration Stations Worksheet Station 1: Overview 1. Why is cellular respiration also known as aerobic respiration? 2. What is the energy currency of the cell? 3. What three components make up cellular respiration? 4. Cellular respiration produces _______ molecules of ATP per ___________ molecule of glucose. 5. Define oxidation and reduction 6. What does LEO the lion says GER refer to? 7. Illustrate the process of oxidation 8. Illustrate the process of reduction 9. Is glucose oxidized or reduced in cellular respiration? 10. What is NAD+ and what happens to it? Where does it get its electrons? 11. What is the terminal electron accepter, and in what part of the cellular respiration cycle does it occur? What element in humans is the terminal electron acceptor? 12. Why do humans need oxygen? Station 2: Glycolysis 1. What substance is glucose broken down into? 2. One glucose __two______ ______________________ ____________________________________ + _______ ATP molecules and two ___________. 3. True or false? If false, make it so that the answer is true: Glycolysis occurs in the mitochondria. 4. Is oxygen needed in order for glycolysis to occur? 5. Fill in the blanks below with regards to the steps of glycolysis: Step 1: Glucose is phosphorylated with _____________ phosphates; these phosphates come from two _____________ molecules. Step 2: The glucose-phosphate molecule splits into _________ sugar molecules, each with three _______________ and one phosphate. Step 3: The 3-carbon phosphate sugars become _____________________, which means they ______________ electrons. These electrons go to NAD+, which become NADH. NADH will be converted into _____________ later. Step 4: An enzyme attaches phosphates to the two ________________ sugars; these phosphates come from a supply in the cytoplasm, not _____________. Step 5: The two newly added phosphates on the 2-carbon sugars removed to make more ___________. Step 6. The remaining phosphates on the 3-carbon sugars are removed to make two more ATP and the two remaining molecules then become _________________. 6. List the three final products of glycolysis and how many of each is formed from one molecule of glucose. 7. Diagram the glycolysis chart on a separate sheet of paper and attach it to this worksheet. Make sure to label the energy investment, energy payoff and the net production phases. Station 3: The Mitochondrion 1. The ______________ membrane of the mitochondrion is made of the ___________________ bilayer. 2. The inner membrane is a _______________ membrane that’s on the inside of the mitochondrion. The ______________________________________ space is the space between the inner and outer membranes. The mitochondrial matrix is the space on the ______________________ of the inner membrane. 3. _________________ are large wrinkles formed by the folding of the ____________________ membrane. The cristae provide a ________________ surface area for cellular respiration, especially in the __________________ _______________________ _________________. 4. Draw and label a picture of a mitochondrion. Include the following: outer membrane, inner membrane, innermembrane space, mitochondrial matrix, cristae, DNA Station 4: Citric acid cycle/Krebs cycle 1. If ___________________ is present in a cell, ___________________ from the cytoplasm in glycolysis will move into the _________________________ for the citric acid cycle. 2. However, when oxygen is NOT present, then pyruvate, there is ________________________ instead of the citric/Krebs cycle. 3. In the citric acid cycle, pyruvate is broken down into _________________ gas, more ATP, NADH, and _________________. 4. In the transitional step, pyruvate loses one molecule of _______________. This step occurs in the ____________________________________. NAD+ oxidizes pyruvate so that it turns into ________________________. Acetate is joined by ________________________________, making acetyle CoA. 5. Steps of the citric acid cycle Step 1: Acetyle CoA loses its acetyl group, which has 2 _____________, and the carbons combine with a 4-carbon compound called ________________________________________ (Dr. Cao may quiz you on that) to make _____________ __________________. Step 2: Citric acid is ___________________________ and loses a __________________ ___________________ molecule twic such that the lost electrons each reduce ______________ into _________________. Thus, one molecule of citric acid produces 2 _________________. Step 3: Dr. Cao does not expect you to remember the rest of the cycle…just know that oxaloacetate is formed again in the cycle, this keeps the cycle going (hence….citric acid cycle…) 6. But do list what products are ultimately produced and how many (i.e., how many ATP, NADH, etc) Station 5: Oxidative phosphorylation 1. Oxidative phosphorylation occurs in the ______________ ____________________________. 2. 1This is where most of the ATP comes from in cellular respiration. The molecules ______________________ and ______________________ are converted into ATP here. 3. This process involves _______________ reactions and ___________________ additions. 4. The two steps of oxidative phosphorylation are: Electron transport chain: no ________here, just a lot of ______________ transport Chemiosmosis: gradient of protons (_______) drive the enzyme ______________ _____________________ to make ______________. 5. In the ETC, _______________ and ____________________ donate _________________ to protein complexes, numbered I, II, ______, and IV. One of the electron carriers, ___________________________, is a lipid. __________________ is the terminal electron acceptor. Oxygen takes electrons from ___________________ or ______________________, then also picks up two _____________________________ ions to make ________________. 6. Since electrons and _____________________ travel together, all this electron shuffling result in some loose protons (___) floating around, and they end end up in the _____________________________ space. 7. What happens at the end of the ETC chain? And what does this power? 8. What is chemiososmosis? 9. What does ATP synthase make ATP from? 10. How many ATP molecules are produced in oxidative phosphorylation? Station 6: Anaerobic respiration and fermentation 1. 2. 3. 4. Cells use anaerobic respiration under what conditions? Is there an electron acceptor involved in anaerobic respiration? Do we use anaerobic respiration? ______________________ extends glycolysis with extra reactions that replenish __________________, so that it can keep going and producing small amounts of _______. 5. What are the two types of fermentation? 6. Lactic acid fermentation occurs in _________________, fungi, and ______________ cells. Pyruvate molecules are reduced to __________________, while NADH is oxidized to ___________________, which replenishes NAD+ for glycolysis. 7. Alcohol fermentation is similar to lactic acid fermentation, except that pyruvate is reduced to ___________________ and produces two ____________ ________________ molecules. Bacteria and _______________ undergo alcohol fermentation.