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Download ISVEE/181 Molecular characterization of indigenous peste des petits
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ISVEE/181 Molecular characterization of indigenous peste des petits ruminants virus by IcELISA, sequencing and phylogenetic analysis of nucleoprotein and fusion protein genes of Pakistan Haider Ali Khan, Muhammad Siddique, Muhammad Khalid Mansoor, Muhammad Jamal, Sajjad-ur-Rahman and Muhammad Javed Arshad Department of Veterinary Microbiology, University of Agricultural, Faisalabad, Pakistan1National Veterinary Laboratory, Islambad, Pakistan Peste des petits ruminants (PPR) is an important viral disease of sheep and goats, endemic in India. The study was undertaken to characterize the local PPRV by the detection of antigen by IcELISA and sequencing of fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. A total of 64 clinical samples collected from sheep and goats. The samples were positive with IcELISA were also found positive with RT-PCR. Selected regions of PPRV genome were amplified from by RT-PCR and the resulting amplicons were sequenced for phylogenetic analysis. The phylogenetic tree based on the 308 bp F gene sequences of PPRV from two different locations revealed a different pattern of branching, yielding two distinct clusters for Nigerian, Turkey and Indian isolates. While the 238 bp N gene sequences clustered them into lineage 4 along with other Asian isolates. Thus, classification of PPRV into lineages based on the F gene sequences appeared to yield better picture of molecular epidemiology for PPRV.