Download ISVEE/181 Molecular characterization of indigenous peste des petits

ISVEE/181
Molecular characterization of indigenous peste des petits ruminants virus by
IcELISA, sequencing and phylogenetic analysis of nucleoprotein and fusion protein
genes of Pakistan
Haider Ali Khan, Muhammad Siddique, Muhammad Khalid Mansoor, Muhammad
Jamal, Sajjad-ur-Rahman and Muhammad Javed Arshad
Department of Veterinary Microbiology, University of Agricultural, Faisalabad,
Pakistan1National Veterinary Laboratory, Islambad, Pakistan
Peste des petits ruminants (PPR) is an important viral disease of sheep and
goats, endemic in India. The study was undertaken to characterize the local
PPRV by the detection of antigen by IcELISA and sequencing of fusion (F) protein
and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus
on genetic variation in the field viruses. A total of 64 clinical samples collected
from sheep and goats. The samples were positive with IcELISA were also found
positive with RT-PCR. Selected regions of PPRV genome were amplified from by
RT-PCR and the resulting amplicons were sequenced for phylogenetic analysis.
The phylogenetic tree based on the 308 bp F gene sequences of PPRV from two
different locations revealed a different pattern of branching, yielding two distinct
clusters for Nigerian, Turkey and Indian isolates. While the 238 bp N gene
sequences clustered them into lineage 4 along with other Asian isolates. Thus,
classification of PPRV into lineages based on the F gene sequences appeared to
yield better picture of molecular epidemiology for PPRV.