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Unit 3 Transport of Materials Key Questions 1. Why must materials enter and leave cells? 2. What materials need to enter and leave cells? 3. What role does the cell membrane play in the transport of materials into and out of a cell? 4. How do materials get into and out of a cell? 5. How do materials move within a cell? Transport 1. Why must materials constantly enter and leave cells? Transport Cells must maintain a constant internal environment (homeostasis) So, materials (nutrients and wastes) must constantly move in and out of the cell and throughout the cell. Transport 2. What materials need to enter and leave cells? Transport Examples of substances entering a cell: Nutrients Salts Oxygen Water Examples of substances leaving a cell: Wastes Carbon dioxide Synthesized molecules Water Transport 3. What role does the cell membrane play in the transport of materials into and out of a cell? Transport Cell Membrane: The cell membrane is selectively permeable The cell membrane regulates/controls what is transported into (absorption) and out of the cell Cell Membrane Certain substances can pass through the membrane more easily than others, Small molecules pass easily (ex.: water, glucose, amino acids, carbon dioxide, oxygen) Large molecules cannot pass easily (ex.: starch, proteins) Cell Membrane That is why they get broken down during DIGESTION!!! Cell Membrane Absorption is when nutrients (like glucose and oxygen) are taken into a cell The cell membrane has a large surface area in order for the absorption of nutrients to occur Transport 4. How do materials get into and out of a cell? DEMO-room spray Transport By diffusion/osmosis or active transport!!! 1. Diffusion/Osmosis Does NOT require ENERGY to move materials into and out of the cell. Diffusion Movement of molecules from a high concentration to a low concentration due to a concentration gradient. Concentration Gradient – difference between high and low Diffusion Occurs because the molecules are constantly moving. Continues until equilibrium is reached – when the molecules are distributed evenly. Diffusion Animation http://highered.mcgrawhill.com/sites/0072495855/student_view0/chapter 2/animation__how_diffusion_works.html Balloons and Diffusion Why do helium balloons sink over time? DEMO: Why can you smell the flavor through the balloon? 2. Osmosis The diffusion of water molecules through a semi-permeable membrane Occurs from an area with a high concentration of water molecules to an area with a low concentration of water molecules DEMO: Beaker and food coloring! Effects of Osmosis on Cells Isotonic Solution: Concentration of water molecules inside the cell and in an isotonic solution solution is the same No concentration gradient, so cell doesn’t gain or lose water Isotonic Solution - Animal Cell Isotonic Solution – Plant Cell Solute concentration inside the cell is equal to outside Effects of Osmosis on Cells Hypotonic Solution Larger concentration of water molecules outside the cell Solution has less dissolved materials than the cell Water moves into the cell by osmosis and animal cells swell and could burst Hypotonic Solution – Animal Cell Hypotonic Solution – Plant Cell Effects of Osmosis on Cells Hypertonic Solution Larger concentration of water molecules inside the cell Solution has more dissolved materials than the cell Water moves out by osmosis and animal cells shrink Hypertonic Solution – Animal Cell Hypertonic Solution – Plant Cell Osmosis Vocab for Plant Cells Turgor Pressure Pressure on cell wall caused by increased expansion of water in vacuole from osmosis The rigid cell wall prevents cell from bursting Plasmolysis When in hypertonic solutions, cell membrane pulls away from cell wall due to water loss by osmosis 2. Active Transport Processes that do require use of energy to move materials into and out of cells Important in movement of molecules into and out of cells Active Transport Move materials from a high concentration to a low concentration OR from a low to a high concentration (against concentration gradient) Moves larger molecules FYI – Types of Active Transport 1. Membrane/Transport Pump Carrier proteins are used to pick up ions or molecules near the cell membrane, carry them across the membrane, and release them on the other side. Types of Active Transport 2. Endocytosis/ Exocytosis Cell membrane folds in and forms a small pouch Pouch releases the contents inside the cell Exocytosis is the opposite Inside the Cell Once materials are inside the cell, they can move throughout by: Diffusion in the cytoplasm Cyclosis – streaming of the cytoplasm – to move materials throughout the cell And the endoplasmic reticulum and golgi body Cyclosis in Plant Cells http://www.dnatube.com/video/361/Cyclosis-inPlant-cells NY State Required LabDiffusion Through a Membrane Objectives – Week 1: Testing with chemical indicators Understanding diffusion (size of molecules) Results-part 1 Explain the ‘final state.’’ Which molecules diffused and why? Which molecules did not diffuse and why? Part 2-Osmosis Objectives: Explain what happens when cells are placed in solutions having different concentrations Explain osmosis Explain what happened to the cells and why. Applicable NY State Standards 1.2g, 1.2h, 1.2i