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2/28/12 • What is a halophile? • Key Term: archaea 3/1/12 • What compound makes up the cell wall of a bacterium? • Key terms: same as yesterday Prokaryotes • Domain Bacteria • Domain Archaebacteria Domain Bacteria Domain Archaea 2007-2008 Common ancestor Domain Eukarya Bacteria Structures Bacteria Structures/Functions Structure Function Flagella Tail-like structure for movement Pili Projection of cell wall for sticking to surfaces Cell wall Maintains shape of cell; protects the cell Cell membrane Regulates what leaves and enters the cell DNA Stores hereditary information Ribosomes Builds proetins Bacteria Structures/Functions Structure Flagella Pili Cell wall Cell membrane DNA Ribosomes Function Prokaryote Structure • Unicellular – bacilli, cocci, spirilli • Size – 1/10 size of eukaryote cell • 1 micron (1um) • Internal structure – no internal compartments • no membrane-bound organelles • only ribosomes – circular, naked DNA • not wrapped around proteins prokaryote cell Bacterial diversity rods and spheres and spirals… Oh My! Bacteria live EVERYWHERE! • Bacteria live in all ecosystems – on plants & animals – in plants & animals – in the soil – in depths of the oceans – in extreme cold – in extreme hot – in extreme salt – on the living – on the dead Archaebacteria • Extremophiles: – Thermoacidophiles – love heat (up to 110’C) and acidic environments; require sulfur; anaerobic ex: Sulfolobus, Pyrodictium – Halophiles –love environments with high salt concentrations like the Great Salt Lake and the Dead Sea; nearly all aerobic; all Gram-negative ex: Halococcus – Methanogens – anaerobic methane producers; use carbon dioxide; live in soil, swamps, digestive tracts ex: Methanococcus Genetic variation in bacteria • Mutations – bacteria can reproduce every 20 minutes • binary fission – error rate in copying DNA • 1 in every 200 bacteria has a mutation • Genetic recombination – bacteria swap genes • plasmids – small supplemental circles of DNA Prokaryotic metabolism • How do bacteria acquire their energy & nutrients? – photoautotrophs • photosynthetic bacteria – chemoautotrophs • oxidize inorganic compounds – nitrogen, sulfur, hydrogen… – heterotrophs • live on plant & animal matter • decomposers & pathogens Genetic Recombination Type Source of New genes Entry method Transformation DNA in surroundings Endocytosis Transduction Virus w/bacterial DNA Viral mediated entry Conjugation Other bacteria Pili bridge http://www.phschool.com/science/biology_place/labbench/lab6/con cepts1.html http://highered.mcgrawhill.com/sites/0072556781/student_view0/chapter13/animation_quiz_2. html http://www.hhmi.org/biointeractive/animations/conjugation/conj_frames .htm Prokaryote Cell Wall Structure Gram-positive bacteria peptide side chains cell wall peptidoglycan plasma membrane protein That’s peptidoglycan = polysaccharides + amino acid chains important for lipopolysaccharides = lipids + polysaccharides your doctor to know! outer membrane of Gram-negative bacteria lipopolysaccharides cell wall outer membrane peptidoglycan plasma membrane Variations in Cell Interior cyanobacterium (photosythetic) bacterium aerobic bacterium Bacteria as pathogens Disease-causing microbes plant diseases wilts, fruit rot, blights animal diseases tooth decay, ulcers anthrax, botulism plague, leprosy, “flesheating” disease STDs: gonorrhea, chlamydia typhoid, cholera TB, pneumonia lyme disease Do you recognize…? Bacteria as beneficial (& necessary) • Life on Earth is dependent on bacteria – decomposers • recycling of nutrients from dead to living – nitrogen fixation • only organisms that can fix N from atmosphere – needed for synthesis of proteins & nucleic acids – plant root nodules – help in digestion (E. coli) • digest cellulose for herbivores – cellulase enzyme • produce vitamins K & B12 for humans – produce foods & medicines • from yogurt to insulin Comparison of prokaryotes and eukaryotes… • Describe three ways in which prokaryotes are similar to eukaryotes. • Explain the size difference in prokaryotes and eukaryotes. • Prokaryotes and eukaryotes have three structures in common. Both have cell membranes that regulate what leaves and enters the cell. Ribosomes, which make proteins, are found in both types of cells. The cytoplasm, a fluid filled with ions and water, surrounds the internal structures in prokaryotes and eukaryotes. DNA is present in both cells however, in eukaryotes it is contained in a nucleus. • Eukaryotes are 10 times larger than prokaryotic cells. They have many internal compartments (organelles) that prokaryotes do not. The Theory of Endosymbiosis suggests that larger cells “ate” bacteria cells and didn’t digest them so they became larger. Pathogenic Bacteria Name of bacteria Can you predict the shape from name? Gram-Stain (+ = purple, = pink) Shape Notes