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The University of Michigan Protein Structure Facility 2560 MSRB II, Box 5674 1150 W. Medical Center Dr. Ann Arbor, MI 48109-5674 Protein Mass Spectrometry Service Fees for MDRTC (effective January 1, 2008) Molecular Weight Measurement by Electrospray-MS Synthetic Peptides (LCQ Duo)) Molecular Weight of Intact Proteins (Qtof), small proteins (<25kD) Molecular Weight of Intact Proteins (Qtof), proteins larger than 25kD Micro-Desalting Ann Arbor, MI 48109-5674 Telephone: (734) 763-6285 Fax: (734) 936-2633 [email protected] Director: Henriette A Remmer, Ph.D. Fee per sample $6 $20 $29 $3 Molecular Weight Measurement by MALDI-TOF-MS Molecular Weight of Peptides Molecular Weight of Proteins Micro-Desalting $8 $16 $3 Protein Sequencing and Identification Individual Services Scientific Consultation required prior to preparing samples for submission SDS-PAGE with high sensitivity colloidal Coomassie Blue stain (sample preparation) LC-MS analysis LC-MS/MS analysis Database search and report Maximum recovery In-gel digestion (Trypsin)*, 1st sample of gel Maximum recovery In-gel digestion (Trypsin)*, sample 2-10 of same gel Peptide Mass Fingerprinting (MALDI-TOF), database search results**, 1st sample Peptide Mass Fingerprinting, (MALDI-TOF) database search results**, sample 2-10 of same gel Detailed data or spectrum analysis and/or detailed data interpretation (per hour) *other proteases are available at additional cost; please inquire ** validated database search results without interpretation $16 $36 $49 $12 $17 $11 $11 $10 $18 Proetin Sequencing and Identification Protein ID Packages Scientific Consultation required prior to preparing samples for submission Protein ID package LC-MS/MS Highest mass accuracy and sensitivity; protein mixtures, maximum confidence and de-novo sequencing Includes: SDS-PAGE with colloidal coomassie stain, maximum recovery in-gel digest, LC-MS/MS and database search 1st sample of batch/gel 2nd to 10th sample of same batch/gel Protein ID package Peptide Mass Fingerprinting $93 $72 Single component protein samples, high sensitivity Includes: SDS-PAGE with colloidal coomassie stain, maximum recovery in-gel digest, Peptide Mass Fingerprinting MALDI-TOF-MS, and database search 1st sample of batch/gel 2nd to 10th sample of same batch/gel Protein ID package Peptide Mass Fingerprinting (Economy Option) $55 $32 Single component protein samples, submit proteins in-gel Includes: Maximum recovery in-gel digest, Peptide Mass Fingerprinting MALDI-TOF-MS, and validated database search results without interpretation 1st sample of batch/gel nd th 2 to 10 sample of same batch/gel $40 $32 Policy: Samples will not be processed before request form has been completed and shortcode has been provided. Cancellations are not accepted after analysis, synthesis or preparation steps have been started. In case of an undesired result due to the nature of the submitted sample or peptide sequence, fees still apply. Note: Members of the University of Michigan Diabetes Research and Training Center (MDRTC), Rheumatic Disease Core Center (RDCC) receive a discount on Protein Structure Facility mass spectrometry services.