Download Mouse anti-receptor protein tyrosine phosphatase sigma, RPTPσ

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Mouse anti-receptor protein tyrosine phosphatase sigma, RPTPσ (17G7.2), #MM-0020
DATASHEET
Product name: receptor protein tyrosine phosphatase sigma (RPTPσ) antibody
Background information: Type IIa receptor protein tyrosine phosphatases (RPTPs) are a group of well-characterized
proteins that are involved in axon growth and guidance during neural development. Members of this subfamily, RPTPσ,
RPTPδ and LAR contain two cytoplasmic phosphatase domains and extracellular immunoglobulin domains followed by
fibronectin type III repeats. RPTPσ is a type I transmembrane protein that has an apparent molecular weight of
approximately 80 kDa. It is highly expressed by neurons in developing and adult mammalian nervous system. Knock-out
mice for RPTPσ show growth retardation, delayed peripheral nerve development and high mortality rates. The role of
RPTPσ in axon guidance during development suggests it could influence axon regeneration after injury in the adult
mouse. It has been shown that RPTPσ slows axon regeneration in the adult injured CNS.
Product description: A highly specific and sensitive antibody against receptor protein tyrosine phosphatase sigma. The
antibody is in a protein-free culture medium.
Format: 100 µg of lyophilized antibody. Reconstitute in 100 µl of distilled H2O for a 1 µg/µl solution. It contains no
additives.
Species: Mouse
Clonality: Monoclonal
Isotype: IgG1, Kappa chain
Reactivity / specificity: The antibody recognizes the intracellular sub-unit of RPTPσ. Specific
for: human, monkey and mouse; other species not tested.
Applications: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended starting dilutions: WB: 1:2000 if reconstituted in 100 µl. Optimal dilution has
to be determined by the user.
Western blot analysis of RPTPσ
expression in newborn mouse
brain lysates.
RPTPσ wild type (+/+) and knock
out (-/-).
Storage: Lyophilized antibody can be kept at 4ºC for up to 3 months and should be kept at -20ºC
for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted
before freezing for short-term storage (-20ºC) or for long-term storage (-80ºC). For maximum recovery of product,
centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Stability: Minimum 1 year from reception date.
References:
1. Kurihara, D., and Yamashita, T. (2012). Chondroitin sulfate proteoglycans down-regulate spine formation in
cortical neurons by targeting tropomyosin-related kinase B (TrkB) protein. J Biol Chem 287, 13822-13828.
2. Sapiehaa, P.S., Duplana, L., Uetanib, N., Jolya, S., Tremblayb, M.L., Kennedyc, T.E., and Polo, A.D. (2005).
Receptor protein tyrosine phosphatase sigma inhibits axon regrowth in the adult injured CNS. Mol Cell Neurosci
28, 625-635.
3. Takahashi, H., Arstikaitis, P., Prasad, T., Bartlett, T.E., Wang, Y.T., Murphy, T.H., and Craig, A.M. (2011).
Postsynaptic TrkC and presynaptic PTPσ function as a bidirectional excitatory synaptic organizing complex.
Neuron 69, 287-303.
4. Thompsona, K.M., Uetanib, N., Manitta, C., Elcheblyc, M., Tremblayb, M.L., and Kennedy, T.E. (2003). Receptor
protein tyrosine phosphatase sigma inhibits axonal regeneration and the rate of axon extension. Mol Cell
Neurosci 23, 681-692.
Limitations: This product is to be used for research purposes only.