Download Human/Mouse/Rat ILKAP/PP2Cδ Antibody

Human/Mouse/Rat ILKAP/PP2Cδ Antibody
Monoclonal Mouse IgG2B Clone # 499526
Catalog Number: MAB4554
DESCRIPTION
Species Reactivity
Human/Mouse/Rat
Specificity Detects endogenous human, mouse and rat ILKAP in Western blots. In Western blots, this antibody does not cross­react with recombinant human PP2Cα, PP2Cβ or PPC2γ. Source
Monoclonal Mouse IgG2B Clone # 499526
Purification Protein A or G purified from hybridoma culture supernatant Immunogen E. coli­derived recombinant human ILKAP Met1­His392 Accession # Q9H0C8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (­SP) is supplied as a 0.2 μm filtered solution in PBS.
APPLICATIONS
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Recommended Concentration
Sample
Western Blot
1 µg/mL
See Below
DATA Western Blot
Detection of Human ILKAP/PP2Cδ by Western Blot. Western blot shows lysates of PC­3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cδ Monoclonal Antibody (Catalog # MAB4554) followed by HRP­conjugated Anti­Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cδ at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. PREPARATION AND STORAGE Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (­SP) is shipped with polar packs. Upon receipt, store it immediately at ­20 to ­70 °C Stability & Storage
Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution. BACKGROUND Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cδ, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK­3β pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers. Rev. 10/13/2015 Page 1 of 1