Download Mouse anti-D4-GDI - Thermo Fisher Scientific

Qty: 100 μg/200 μl
Mouse anti-D4-GDI
Catalog No. 35-9900
Lot No.
Mouse anti-D4-GDI
FORM
This monoclonal antibody is supplied as a 200 μl aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing
0.1% sodium azide. This antibody is highly purified from mouse ascites by protein A chromatography.
CLONE: 2H7F3
ISOTYPE: IgG1
IMMUNOGEN
Recombinant D4-GDI fusion protein.
SPECIFICITY
This antibody reacts with the full-length form of the human D4-GDI protein. Cross-reactivity with Rho-GDI has not been
tested. On Western blots, this antibody identifies a band at 28 kDa.
REACTIVITY
Reactivity has been confirmed with human Jurkat cell lysates.
Sample
Human
Mouse
Immunogen
ELISA
ND
ND
+++
Western
Blotting
+++
ND
N/A
(Excellent +++, Good++, Poor +, No reactivity 0, Not applicable N/A, Not Determined ND)
USAGE
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be
affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method,
temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended starting points for this product.
ELISA : 0.1 – 1.0 μg/mL
Western Blotting: 1-3 μg/mL
STORAGE
Store at 2-8°C for up to one month. Store at –20°C for long-term storage. Avoid repeated freezing and thawing.
(cont’d)
www.invitrogen.com
Invitrogen Corporation • 1600 Faraday Avenue • Carlsbad • CA 92008 • Tel: 800.955.6288 • E-mail: [email protected]
PI35-9900
Rev. 7/07
Copyright 2005, Zymed Laboratories
DCC-06-0084
(35-9900 cont’d)
BACKGROUND
Guanine nucleotide dissociation inhibitor (GDI) proteins exert negative regulatory effects on the Rho family of GTPases
(Rho, Rac, Cdc42) by inhibiting the dissociation of GDP, thereby maintaining GTPases in their inactive, cytosolic form. The
Rho family of GTPases is involved in a diverse variety of cellular functions, including cell signaling, proliferation, cytoskeletal
organization, and secretion.1-6 These proteins also regulate specific aspects of inflammatory activity, such as motility,
3
production of pro-inflammatory cytokines, and generation of toxic oxygen metabolites.
D4-GDI (or LY-GDI) was originally identified as a hematopoeitic cell-abundant homolog of Rho-GDI.7,8 Unlike Rho-GDI,
which is ubiquitously expressed, D4-GDI is predominantly expressed by lymphoid and myeloid cells. The amino acid
9
sequence of D4-GDI predicts a ~23 kDa protein; however, the full-length protein migrates at ~28 kDa by SDS-PAGE.
Studies have demonstrated that D4-GDI is a proteolytic substrate for caspase-3 and –8, and that cleavage by either
caspase-3 or –8 can produce de-regulation of Rho-GTPases.9,10 Release of Rho-GTPases from the inhibitory constraints of
D4-GDI likely results in cytoskeletal alterations, activation of the stress-activated protein kinases p38 and c-Jun, and
enhanced production of toxic oxygen metabolites.
REFERENCES
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Hall A. Ann Rev Cell Biol 10:31-54, 1994.
Tapon N, Hall A. Curr Opin Cell Biol 9:86-92, 1997.
Dharmawardhane S, Bokoch GM. Curr Opin Hematol 4:12-18, 1997.
Ridley AJ. Curr Biol 6:1256-1264, 1996.
Denhardt DT. Biochem J 318:729-747, 1996.
Symons M. Trends Biochem Sci 21:178-181, 1996.
Lelias JM, et al. PNAS 90:1479-1483, 1993.
Scherle P, et al. PNAS 90:7568-7572, 1993.
Na S, et al. J Biol Chem 271:11209-11213, 1996.
Danley DE, et al. J Immunol 157:500-503, 1996.
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Zymed® and ZyMAX™ are trademarks of Zymed Laboratories Inc. Cy™ is a trademark of Amersham Biosciences Ltd. Sepharose® is a
registered trademark of Pharmacia LKB.
For Research Use Only
LF021025
www.invitrogen.com
Invitrogen Corporation • 1600 Faraday Avenue • Carlsbad • CA 92008 • Tel: 800.955.6288 • E-mail: [email protected]
PI35-9900
Rev. 7/07
Copyright 2005, Zymed Laboratories
DCC-06-0084